Molecular characterization of paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) isolates.

Detalhes bibliográficos
Autor(a) principal: AZEVEDO, A. C. S.
Data de Publicação: 2000
Outros Autores: FURLANETO, M. C., SOSA-GOMEZ, D. R., FUNGARO, M. H. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/952773
Resumo: ABSTRACT ITS and RAPD analyses were used to investigate molecular variations within samples of Paecilomyces isolates and to resolve five morphologically atypical isolates resembling P. fumosorosus, obtained from whitefly in Northern Paraná State. The ITS4-ITS5 amplicon was 700 base pairs (bp) long in all isolates. The five isolates of Paecilomyces not assigned to species produced restriction profiles identical to all the reference strains of P. fumosoroseus. The extent of fingerprint variability observed by RAPD was sufficient to discriminate all the isolates. The genetic similarity among unidentified isolates and strains of P. fumosoroseus was even higher than that observed among reference strains of this species, allowing us to conclude that isolates CNPso-P77, CNPso-P78, CNPso-P80, CNPso-P85 and CNPso-P91 are P. fumosoroseus. Key words: Paecilomyces fumosoroseus, Bemisia, RAPD, biological control, entomopathogenic fungus
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spelling Molecular characterization of paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) isolates.SojaABSTRACT ITS and RAPD analyses were used to investigate molecular variations within samples of Paecilomyces isolates and to resolve five morphologically atypical isolates resembling P. fumosorosus, obtained from whitefly in Northern Paraná State. The ITS4-ITS5 amplicon was 700 base pairs (bp) long in all isolates. The five isolates of Paecilomyces not assigned to species produced restriction profiles identical to all the reference strains of P. fumosoroseus. The extent of fingerprint variability observed by RAPD was sufficient to discriminate all the isolates. The genetic similarity among unidentified isolates and strains of P. fumosoroseus was even higher than that observed among reference strains of this species, allowing us to conclude that isolates CNPso-P77, CNPso-P78, CNPso-P80, CNPso-P85 and CNPso-P91 are P. fumosoroseus. Key words: Paecilomyces fumosoroseus, Bemisia, RAPD, biological control, entomopathogenic fungusANDRÉIA CRISTIANE SOUZA AZEVEDO; MÁRCIA CRISTINA FURLANETO; DANIEL RICARDO SOSA GOMEZ, CNPSO; MARIA HELENA PELEGRINELLI FUNGARO.AZEVEDO, A. C. S.FURLANETO, M. C.SOSA-GOMEZ, D. R.FUNGARO, M. H. P.2013-03-12T11:11:11Z2013-03-12T11:11:11Z2013-03-1220002018-08-09T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleScientia Agrícola, Piracicaba, [online]. v.57, n.4, p. 729-732, 2000.0103-9016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/952773enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:23:41Zoai:www.alice.cnptia.embrapa.br:doc/952773Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:23:41falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:23:41Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Molecular characterization of paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) isolates.
title Molecular characterization of paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) isolates.
spellingShingle Molecular characterization of paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) isolates.
AZEVEDO, A. C. S.
Soja
title_short Molecular characterization of paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) isolates.
title_full Molecular characterization of paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) isolates.
title_fullStr Molecular characterization of paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) isolates.
title_full_unstemmed Molecular characterization of paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) isolates.
title_sort Molecular characterization of paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) isolates.
author AZEVEDO, A. C. S.
author_facet AZEVEDO, A. C. S.
FURLANETO, M. C.
SOSA-GOMEZ, D. R.
FUNGARO, M. H. P.
author_role author
author2 FURLANETO, M. C.
SOSA-GOMEZ, D. R.
FUNGARO, M. H. P.
author2_role author
author
author
dc.contributor.none.fl_str_mv ANDRÉIA CRISTIANE SOUZA AZEVEDO; MÁRCIA CRISTINA FURLANETO; DANIEL RICARDO SOSA GOMEZ, CNPSO; MARIA HELENA PELEGRINELLI FUNGARO.
dc.contributor.author.fl_str_mv AZEVEDO, A. C. S.
FURLANETO, M. C.
SOSA-GOMEZ, D. R.
FUNGARO, M. H. P.
dc.subject.por.fl_str_mv Soja
topic Soja
description ABSTRACT ITS and RAPD analyses were used to investigate molecular variations within samples of Paecilomyces isolates and to resolve five morphologically atypical isolates resembling P. fumosorosus, obtained from whitefly in Northern Paraná State. The ITS4-ITS5 amplicon was 700 base pairs (bp) long in all isolates. The five isolates of Paecilomyces not assigned to species produced restriction profiles identical to all the reference strains of P. fumosoroseus. The extent of fingerprint variability observed by RAPD was sufficient to discriminate all the isolates. The genetic similarity among unidentified isolates and strains of P. fumosoroseus was even higher than that observed among reference strains of this species, allowing us to conclude that isolates CNPso-P77, CNPso-P78, CNPso-P80, CNPso-P85 and CNPso-P91 are P. fumosoroseus. Key words: Paecilomyces fumosoroseus, Bemisia, RAPD, biological control, entomopathogenic fungus
publishDate 2000
dc.date.none.fl_str_mv 2000
2013-03-12T11:11:11Z
2013-03-12T11:11:11Z
2013-03-12
2018-08-09T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Scientia Agrícola, Piracicaba, [online]. v.57, n.4, p. 729-732, 2000.
0103-9016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/952773
identifier_str_mv Scientia Agrícola, Piracicaba, [online]. v.57, n.4, p. 729-732, 2000.
0103-9016.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/952773
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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