Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates

Detalhes bibliográficos
Autor(a) principal: Azevedo,Andréia Cristiane Souza
Data de Publicação: 2000
Outros Autores: Furlaneto,Márcia Cristina, Sosa-Gómez,Daniel Ricardo, Fungaro,Maria Helena Pelegrinelli
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400021
Resumo: ITS and RAPD analyses were used to investigate molecular variations within samples of Paecilomyces isolates and to resolve five morphologically atypical isolates resembling P. fumosorosus, obtained from whitefly in Northern Paraná State. The ITS4-ITS5 amplicon was 700 base pairs (bp) long in all isolates. The five isolates of Paecilomyces not assigned to species produced restriction profiles identical to all the reference strains of P. fumosoroseus. The extent of fingerprint variability observed by RAPD was sufficient to discriminate all the isolates. The genetic similarity among unidentified isolates and strains of P. fumosoroseus was even higher than that observed among reference strains of this species, allowing us to conclude that isolates CNPso-P77, CNPso-P78, CNPso-P80, CNPso-P85 and CNPso-P91 are P. fumosoroseus.
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spelling Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolatesPaecilomyces fumosoroseusBemisiaRAPDbiological controlentomopathogenic fungusITS and RAPD analyses were used to investigate molecular variations within samples of Paecilomyces isolates and to resolve five morphologically atypical isolates resembling P. fumosorosus, obtained from whitefly in Northern Paraná State. The ITS4-ITS5 amplicon was 700 base pairs (bp) long in all isolates. The five isolates of Paecilomyces not assigned to species produced restriction profiles identical to all the reference strains of P. fumosoroseus. The extent of fingerprint variability observed by RAPD was sufficient to discriminate all the isolates. The genetic similarity among unidentified isolates and strains of P. fumosoroseus was even higher than that observed among reference strains of this species, allowing us to conclude that isolates CNPso-P77, CNPso-P78, CNPso-P80, CNPso-P85 and CNPso-P91 are P. fumosoroseus.Escola Superior de Agricultura "Luiz de Queiroz"2000-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400021Scientia Agricola v.57 n.4 2000reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162000000400021info:eu-repo/semantics/openAccessAzevedo,Andréia Cristiane SouzaFurlaneto,Márcia CristinaSosa-Gómez,Daniel RicardoFungaro,Maria Helena Pelegrinellieng2001-02-02T00:00:00Zoai:scielo:S0103-90162000000400021Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2001-02-02T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates
title Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates
spellingShingle Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates
Azevedo,Andréia Cristiane Souza
Paecilomyces fumosoroseus
Bemisia
RAPD
biological control
entomopathogenic fungus
title_short Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates
title_full Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates
title_fullStr Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates
title_full_unstemmed Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates
title_sort Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates
author Azevedo,Andréia Cristiane Souza
author_facet Azevedo,Andréia Cristiane Souza
Furlaneto,Márcia Cristina
Sosa-Gómez,Daniel Ricardo
Fungaro,Maria Helena Pelegrinelli
author_role author
author2 Furlaneto,Márcia Cristina
Sosa-Gómez,Daniel Ricardo
Fungaro,Maria Helena Pelegrinelli
author2_role author
author
author
dc.contributor.author.fl_str_mv Azevedo,Andréia Cristiane Souza
Furlaneto,Márcia Cristina
Sosa-Gómez,Daniel Ricardo
Fungaro,Maria Helena Pelegrinelli
dc.subject.por.fl_str_mv Paecilomyces fumosoroseus
Bemisia
RAPD
biological control
entomopathogenic fungus
topic Paecilomyces fumosoroseus
Bemisia
RAPD
biological control
entomopathogenic fungus
description ITS and RAPD analyses were used to investigate molecular variations within samples of Paecilomyces isolates and to resolve five morphologically atypical isolates resembling P. fumosorosus, obtained from whitefly in Northern Paraná State. The ITS4-ITS5 amplicon was 700 base pairs (bp) long in all isolates. The five isolates of Paecilomyces not assigned to species produced restriction profiles identical to all the reference strains of P. fumosoroseus. The extent of fingerprint variability observed by RAPD was sufficient to discriminate all the isolates. The genetic similarity among unidentified isolates and strains of P. fumosoroseus was even higher than that observed among reference strains of this species, allowing us to conclude that isolates CNPso-P77, CNPso-P78, CNPso-P80, CNPso-P85 and CNPso-P91 are P. fumosoroseus.
publishDate 2000
dc.date.none.fl_str_mv 2000-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-90162000000400021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.57 n.4 2000
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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