Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple.

Detalhes bibliográficos
Autor(a) principal: PORTO, D. D.
Data de Publicação: 2016
Outros Autores: FALAVIGNA, V. da S., ARENHART, R. A., PERINI, P., BUFFON, V., ANZANELLO, R., SANTOS, H. P. dos, FIALHO, F. B., OLIVEIRA, P. R. D. de, REVERS, L. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061885
Resumo: The molecular control of bud dormancy establishment and release is still not well understood, although some genes have already been demonstrated to play important roles in this process. The dormancy-associated MADS-box (DAM) genes were first identified in the peach EVERGROWING locus and are considered the main regulators of bud dormancy control. In this work, the apple (Malus × domestica Borkh.), a perennial plant adapted to temperate climates that displays cycles of growth and bud dormancy, was screened for the presence of DAM genes. The candidate genes retrieved were characterized in comparison to DAM genes from other species. Four of them (MdDAM1?4) are structurally very similar to the reported DAM genes. When apple genomic segments containing these candidates were compared to the peach EVERGROWING locus, a highly conserved noncoding region was detected inside their largest intron. Similar sequences were also identified inside introns of apricot and pear DAM genes. Organ expression patterns revealed that MdDAM1?4 are mainly expressed in dormant buds and seeds, with low transcript accumulation in vegetative structures. In addition, the MdDAM genes showed seasonally oscillating patterns of steady-state messenger RNA (mRNA) levels and were downregulated by artificial chilling. Motif analyses in the promoter and in the intronic conserved region of the MdDAM genes disclosed some clues to the regulation of the expression patterns observed. Possible roles for the conserved intronic sequence in dormancy regulation are discussed.
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spelling Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple.Quebra de dormênciaGenes DAMAppleBud dormancyCollinearityDAM genesGrowth regulatorMaçãRegulador de crescimentoMalus DomesticaThe molecular control of bud dormancy establishment and release is still not well understood, although some genes have already been demonstrated to play important roles in this process. The dormancy-associated MADS-box (DAM) genes were first identified in the peach EVERGROWING locus and are considered the main regulators of bud dormancy control. In this work, the apple (Malus × domestica Borkh.), a perennial plant adapted to temperate climates that displays cycles of growth and bud dormancy, was screened for the presence of DAM genes. The candidate genes retrieved were characterized in comparison to DAM genes from other species. Four of them (MdDAM1?4) are structurally very similar to the reported DAM genes. When apple genomic segments containing these candidates were compared to the peach EVERGROWING locus, a highly conserved noncoding region was detected inside their largest intron. Similar sequences were also identified inside introns of apricot and pear DAM genes. Organ expression patterns revealed that MdDAM1?4 are mainly expressed in dormant buds and seeds, with low transcript accumulation in vegetative structures. In addition, the MdDAM genes showed seasonally oscillating patterns of steady-state messenger RNA (mRNA) levels and were downregulated by artificial chilling. Motif analyses in the promoter and in the intronic conserved region of the MdDAM genes disclosed some clues to the regulation of the expression patterns observed. Possible roles for the conserved intronic sequence in dormancy regulation are discussed.DIOGO DENARDI PORTO, CPATSA; VÍTOR DA SILVEIRA FALAVIGNA, UFRGS; RAFAEL AUGUSTO ARENHART, UFRGS; PÂMELA PERINI, Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul; VANESSA BUFFON, CNPUV; RAFAEL ANZANELLO, Fundação Estadual de Pesquisa Agropecuária, Veranópolis, RS; HENRIQUE PESSOA DOS SANTOS, CNPUV; FLAVIO BELLO FIALHO, CNPUV; PAULO RICARDO DIAS DE OLIVEIRA, CNPUV; LUIS FERNANDO REVERS, CNPUV.PORTO, D. D.FALAVIGNA, V. da S.ARENHART, R. A.PERINI, P.BUFFON, V.ANZANELLO, R.SANTOS, H. P. dosFIALHO, F. B.OLIVEIRA, P. R. D. deREVERS, L. F.2017-01-26T11:11:11Z2017-01-26T11:11:11Z2017-01-2620162017-01-26T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTree Genetics & Genomes, v. 12, n. 3, jun. 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/106188510.1007/s11295-016-1001-3enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:05:54Zoai:www.alice.cnptia.embrapa.br:doc/1061885Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:05:54falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:05:54Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple.
title Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple.
spellingShingle Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple.
PORTO, D. D.
Quebra de dormência
Genes DAM
Apple
Bud dormancy
Collinearity
DAM genes
Growth regulator
Maçã
Regulador de crescimento
Malus Domestica
title_short Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple.
title_full Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple.
title_fullStr Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple.
title_full_unstemmed Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple.
title_sort Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple.
author PORTO, D. D.
author_facet PORTO, D. D.
FALAVIGNA, V. da S.
ARENHART, R. A.
PERINI, P.
BUFFON, V.
ANZANELLO, R.
SANTOS, H. P. dos
FIALHO, F. B.
OLIVEIRA, P. R. D. de
REVERS, L. F.
author_role author
author2 FALAVIGNA, V. da S.
ARENHART, R. A.
PERINI, P.
BUFFON, V.
ANZANELLO, R.
SANTOS, H. P. dos
FIALHO, F. B.
OLIVEIRA, P. R. D. de
REVERS, L. F.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DIOGO DENARDI PORTO, CPATSA; VÍTOR DA SILVEIRA FALAVIGNA, UFRGS; RAFAEL AUGUSTO ARENHART, UFRGS; PÂMELA PERINI, Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul; VANESSA BUFFON, CNPUV; RAFAEL ANZANELLO, Fundação Estadual de Pesquisa Agropecuária, Veranópolis, RS; HENRIQUE PESSOA DOS SANTOS, CNPUV; FLAVIO BELLO FIALHO, CNPUV; PAULO RICARDO DIAS DE OLIVEIRA, CNPUV; LUIS FERNANDO REVERS, CNPUV.
dc.contributor.author.fl_str_mv PORTO, D. D.
FALAVIGNA, V. da S.
ARENHART, R. A.
PERINI, P.
BUFFON, V.
ANZANELLO, R.
SANTOS, H. P. dos
FIALHO, F. B.
OLIVEIRA, P. R. D. de
REVERS, L. F.
dc.subject.por.fl_str_mv Quebra de dormência
Genes DAM
Apple
Bud dormancy
Collinearity
DAM genes
Growth regulator
Maçã
Regulador de crescimento
Malus Domestica
topic Quebra de dormência
Genes DAM
Apple
Bud dormancy
Collinearity
DAM genes
Growth regulator
Maçã
Regulador de crescimento
Malus Domestica
description The molecular control of bud dormancy establishment and release is still not well understood, although some genes have already been demonstrated to play important roles in this process. The dormancy-associated MADS-box (DAM) genes were first identified in the peach EVERGROWING locus and are considered the main regulators of bud dormancy control. In this work, the apple (Malus × domestica Borkh.), a perennial plant adapted to temperate climates that displays cycles of growth and bud dormancy, was screened for the presence of DAM genes. The candidate genes retrieved were characterized in comparison to DAM genes from other species. Four of them (MdDAM1?4) are structurally very similar to the reported DAM genes. When apple genomic segments containing these candidates were compared to the peach EVERGROWING locus, a highly conserved noncoding region was detected inside their largest intron. Similar sequences were also identified inside introns of apricot and pear DAM genes. Organ expression patterns revealed that MdDAM1?4 are mainly expressed in dormant buds and seeds, with low transcript accumulation in vegetative structures. In addition, the MdDAM genes showed seasonally oscillating patterns of steady-state messenger RNA (mRNA) levels and were downregulated by artificial chilling. Motif analyses in the promoter and in the intronic conserved region of the MdDAM genes disclosed some clues to the regulation of the expression patterns observed. Possible roles for the conserved intronic sequence in dormancy regulation are discussed.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-01-26T11:11:11Z
2017-01-26T11:11:11Z
2017-01-26
2017-01-26T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Tree Genetics & Genomes, v. 12, n. 3, jun. 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061885
10.1007/s11295-016-1001-3
identifier_str_mv Tree Genetics & Genomes, v. 12, n. 3, jun. 2016.
10.1007/s11295-016-1001-3
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061885
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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