Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate

Detalhes bibliográficos
Autor(a) principal: Leivas,Gabrielle
Data de Publicação: 2022
Outros Autores: Klumb,Elsa Kuhn, Bianchi,Valmor João, Yamamoto,Robson Ryu, Amarante,Luciano de, Pereira,José Francisco Martins, Herter,Flávio Gilberto, Chaves,Ana Lúcia Soares, Mello-Farias,Paulo Celso de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bragantia
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052022000100402
Resumo: ABSTRACT In the last decades, several biochemical and molecular studies in pear (Pyrus spp.) have revealed processes associated with dormancy establishment and release, leading to the identification of several MADS-box family genes related to bud dormancy regulation. Thus, the aim of this study was to evaluate a preliminary expression pattern of the PpDAM13-1 and PpDAM13-2 MADS-box genes in pear leaf buds (Pyrus pyrifolia Nakai, cultivars Hosui and Nijisseiki; and Pyrus communis L., cultivars Rocha and Santa Maria), grown in the subtropical climate of Southern Brazil. Lateral leaf buds were collected from June to September, and RNA was extracted for evaluation of gene expression by reverse transcription polymerase chain reaction (RT-qPCR). It was found that these two genes decreased the expression as leaf buds are release from dormancy. The Hosui cultivar for both genes and the Nijisseiki cultivar for PpDAM13-2 gene showed peaks of expression in early June, indicating that leaf buds are in a transition dormancy phase. The two European cultivars showed this transition dormancy phase between the end-July and the mid-August. It was concluded that the expression analysis of PpDAM13-1 and PpDAM13-2 genes can be used as a marker to evaluate changes of dormancy phase in pear trees in subtropical climate conditions, and it can be suitable to assist management strategies for overcoming pears’ bud dormancy.
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spelling Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climatePyrus spp.bud dormancywarm winterRT-qPCRgene expressionABSTRACT In the last decades, several biochemical and molecular studies in pear (Pyrus spp.) have revealed processes associated with dormancy establishment and release, leading to the identification of several MADS-box family genes related to bud dormancy regulation. Thus, the aim of this study was to evaluate a preliminary expression pattern of the PpDAM13-1 and PpDAM13-2 MADS-box genes in pear leaf buds (Pyrus pyrifolia Nakai, cultivars Hosui and Nijisseiki; and Pyrus communis L., cultivars Rocha and Santa Maria), grown in the subtropical climate of Southern Brazil. Lateral leaf buds were collected from June to September, and RNA was extracted for evaluation of gene expression by reverse transcription polymerase chain reaction (RT-qPCR). It was found that these two genes decreased the expression as leaf buds are release from dormancy. The Hosui cultivar for both genes and the Nijisseiki cultivar for PpDAM13-2 gene showed peaks of expression in early June, indicating that leaf buds are in a transition dormancy phase. The two European cultivars showed this transition dormancy phase between the end-July and the mid-August. It was concluded that the expression analysis of PpDAM13-1 and PpDAM13-2 genes can be used as a marker to evaluate changes of dormancy phase in pear trees in subtropical climate conditions, and it can be suitable to assist management strategies for overcoming pears’ bud dormancy.Instituto Agronômico de Campinas2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052022000100402Bragantia v.81 2022reponame:Bragantiainstname:Instituto Agronômico de Campinas (IAC)instacron:IAC10.1590/1678-4499.20210211info:eu-repo/semantics/openAccessLeivas,GabrielleKlumb,Elsa KuhnBianchi,Valmor JoãoYamamoto,Robson RyuAmarante,Luciano dePereira,José Francisco MartinsHerter,Flávio GilbertoChaves,Ana Lúcia SoaresMello-Farias,Paulo Celso deeng2022-02-14T00:00:00Zoai:scielo:S0006-87052022000100402Revistahttps://www.scielo.br/j/brag/https://old.scielo.br/oai/scielo-oai.phpbragantia@iac.sp.gov.br||bragantia@iac.sp.gov.br1678-44990006-8705opendoar:2022-02-14T00:00Bragantia - Instituto Agronômico de Campinas (IAC)false
dc.title.none.fl_str_mv Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
title Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
spellingShingle Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
Leivas,Gabrielle
Pyrus spp.
bud dormancy
warm winter
RT-qPCR
gene expression
title_short Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
title_full Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
title_fullStr Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
title_full_unstemmed Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
title_sort Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
author Leivas,Gabrielle
author_facet Leivas,Gabrielle
Klumb,Elsa Kuhn
Bianchi,Valmor João
Yamamoto,Robson Ryu
Amarante,Luciano de
Pereira,José Francisco Martins
Herter,Flávio Gilberto
Chaves,Ana Lúcia Soares
Mello-Farias,Paulo Celso de
author_role author
author2 Klumb,Elsa Kuhn
Bianchi,Valmor João
Yamamoto,Robson Ryu
Amarante,Luciano de
Pereira,José Francisco Martins
Herter,Flávio Gilberto
Chaves,Ana Lúcia Soares
Mello-Farias,Paulo Celso de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Leivas,Gabrielle
Klumb,Elsa Kuhn
Bianchi,Valmor João
Yamamoto,Robson Ryu
Amarante,Luciano de
Pereira,José Francisco Martins
Herter,Flávio Gilberto
Chaves,Ana Lúcia Soares
Mello-Farias,Paulo Celso de
dc.subject.por.fl_str_mv Pyrus spp.
bud dormancy
warm winter
RT-qPCR
gene expression
topic Pyrus spp.
bud dormancy
warm winter
RT-qPCR
gene expression
description ABSTRACT In the last decades, several biochemical and molecular studies in pear (Pyrus spp.) have revealed processes associated with dormancy establishment and release, leading to the identification of several MADS-box family genes related to bud dormancy regulation. Thus, the aim of this study was to evaluate a preliminary expression pattern of the PpDAM13-1 and PpDAM13-2 MADS-box genes in pear leaf buds (Pyrus pyrifolia Nakai, cultivars Hosui and Nijisseiki; and Pyrus communis L., cultivars Rocha and Santa Maria), grown in the subtropical climate of Southern Brazil. Lateral leaf buds were collected from June to September, and RNA was extracted for evaluation of gene expression by reverse transcription polymerase chain reaction (RT-qPCR). It was found that these two genes decreased the expression as leaf buds are release from dormancy. The Hosui cultivar for both genes and the Nijisseiki cultivar for PpDAM13-2 gene showed peaks of expression in early June, indicating that leaf buds are in a transition dormancy phase. The two European cultivars showed this transition dormancy phase between the end-July and the mid-August. It was concluded that the expression analysis of PpDAM13-1 and PpDAM13-2 genes can be used as a marker to evaluate changes of dormancy phase in pear trees in subtropical climate conditions, and it can be suitable to assist management strategies for overcoming pears’ bud dormancy.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052022000100402
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052022000100402
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4499.20210211
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Agronômico de Campinas
publisher.none.fl_str_mv Instituto Agronômico de Campinas
dc.source.none.fl_str_mv Bragantia v.81 2022
reponame:Bragantia
instname:Instituto Agronômico de Campinas (IAC)
instacron:IAC
instname_str Instituto Agronômico de Campinas (IAC)
instacron_str IAC
institution IAC
reponame_str Bragantia
collection Bragantia
repository.name.fl_str_mv Bragantia - Instituto Agronômico de Campinas (IAC)
repository.mail.fl_str_mv bragantia@iac.sp.gov.br||bragantia@iac.sp.gov.br
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