Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.

Detalhes bibliográficos
Autor(a) principal: NAITO, F. Y. B.
Data de Publicação: 2019
Outros Autores: MELO, F. L., FONSECA, M. E. N., SANTOS, C. A. F., CHANES, C. R., RIBEIRO, B. M., GILBERTSON, R. L., BOITEUX, L. S., PEREIRA-CARVALHO, R. de C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120225
Resumo: A new bipartite begomovirus (family Geminiviridae) was detected on cowpea (Vigna unguiculata) plants exhibiting bright golden mosaic symptoms on leaves under field conditions in Brazil. Complete consensus sequences of DNA-A and DNA-B components of an isolate of the virus (PE?088) were obtained by nanopore sequencing and confirmed by Sanger sequencing. The genome components presented the typical genomic organization of New World (NW) begomoviruses. Pairwise sequence comparisons revealed low levels of identity with other begomovirus species previously reported infecting cowpea around the world. Phylogenetic analysis using complete sequences of DNA-A components revealed that the closest relatives of PE?088 (85-87% nucleotide sequence identities) were three legume-infecting begomoviruses from Brazil: bean golden mosaic virus, macroptilium common mosaic virus and macroptilium yellow vein virus. According to the current classification criteria, PE?088 represents a new species in the genus Begomovirus, tentatively named as cowpea bright yellow mosaic virus (CoBYMV).
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spelling Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.Feijao caupiNanoporeCaupiMosca BrancaFeijãoDoençaGeminiviridaeVigna unguiculata subsp. unguiculata var. spontaneaBean golden mosaic virusBegomovirusA new bipartite begomovirus (family Geminiviridae) was detected on cowpea (Vigna unguiculata) plants exhibiting bright golden mosaic symptoms on leaves under field conditions in Brazil. Complete consensus sequences of DNA-A and DNA-B components of an isolate of the virus (PE?088) were obtained by nanopore sequencing and confirmed by Sanger sequencing. The genome components presented the typical genomic organization of New World (NW) begomoviruses. Pairwise sequence comparisons revealed low levels of identity with other begomovirus species previously reported infecting cowpea around the world. Phylogenetic analysis using complete sequences of DNA-A components revealed that the closest relatives of PE?088 (85-87% nucleotide sequence identities) were three legume-infecting begomoviruses from Brazil: bean golden mosaic virus, macroptilium common mosaic virus and macroptilium yellow vein virus. According to the current classification criteria, PE?088 represents a new species in the genus Begomovirus, tentatively named as cowpea bright yellow mosaic virus (CoBYMV).FERNANDA Y. B. NAITO, UNIVERSIDADE DE BRASÍLIA; FERNANDO L. MELO, UNIVERSIDADE DE BRASÍLIA; MARIA ESTHER DE N FONSECA BOITEUX, CNPH; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; CAROLINA R. CHANES, UNIVERSIDADE CATÓLICA DE BRASÍLIA; BERGMANN M. RIBEIRO, UNIVERSIDADE DE BRASÍLIA; ROBERT L. GILBERTSON, UNIVERSITY OF CALIFORNIA; LEONARDO SILVA BOITEUX, CNPH; RITA DE CÁSSIA PEREIRA-CARVALHO, UNIVERSIDADE DE BRASÍLIA.NAITO, F. Y. B.MELO, F. L.FONSECA, M. E. N.SANTOS, C. A. F.CHANES, C. R.RIBEIRO, B. M.GILBERTSON, R. L.BOITEUX, L. S.PEREIRA-CARVALHO, R. de C.2020-02-13T18:16:37Z2020-02-13T18:16:37Z2020-02-1320192020-02-13T18:16:37Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleArchives of Virology, v. 164, n. 7, p. 1907-1910, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/112022510.1007/s00705-019-04254-5enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-02-13T18:16:43Zoai:www.alice.cnptia.embrapa.br:doc/1120225Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-02-13T18:16:43falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-02-13T18:16:43Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.
title Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.
spellingShingle Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.
NAITO, F. Y. B.
Feijao caupi
Nanopore
Caupi
Mosca Branca
Feijão
Doença
Geminiviridae
Vigna unguiculata subsp. unguiculata var. spontanea
Bean golden mosaic virus
Begomovirus
title_short Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.
title_full Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.
title_fullStr Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.
title_full_unstemmed Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.
title_sort Nanopore sequencing of a novel bipartite New World begomovirus infecting cowpea.
author NAITO, F. Y. B.
author_facet NAITO, F. Y. B.
MELO, F. L.
FONSECA, M. E. N.
SANTOS, C. A. F.
CHANES, C. R.
RIBEIRO, B. M.
GILBERTSON, R. L.
BOITEUX, L. S.
PEREIRA-CARVALHO, R. de C.
author_role author
author2 MELO, F. L.
FONSECA, M. E. N.
SANTOS, C. A. F.
CHANES, C. R.
RIBEIRO, B. M.
GILBERTSON, R. L.
BOITEUX, L. S.
PEREIRA-CARVALHO, R. de C.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv FERNANDA Y. B. NAITO, UNIVERSIDADE DE BRASÍLIA; FERNANDO L. MELO, UNIVERSIDADE DE BRASÍLIA; MARIA ESTHER DE N FONSECA BOITEUX, CNPH; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; CAROLINA R. CHANES, UNIVERSIDADE CATÓLICA DE BRASÍLIA; BERGMANN M. RIBEIRO, UNIVERSIDADE DE BRASÍLIA; ROBERT L. GILBERTSON, UNIVERSITY OF CALIFORNIA; LEONARDO SILVA BOITEUX, CNPH; RITA DE CÁSSIA PEREIRA-CARVALHO, UNIVERSIDADE DE BRASÍLIA.
dc.contributor.author.fl_str_mv NAITO, F. Y. B.
MELO, F. L.
FONSECA, M. E. N.
SANTOS, C. A. F.
CHANES, C. R.
RIBEIRO, B. M.
GILBERTSON, R. L.
BOITEUX, L. S.
PEREIRA-CARVALHO, R. de C.
dc.subject.por.fl_str_mv Feijao caupi
Nanopore
Caupi
Mosca Branca
Feijão
Doença
Geminiviridae
Vigna unguiculata subsp. unguiculata var. spontanea
Bean golden mosaic virus
Begomovirus
topic Feijao caupi
Nanopore
Caupi
Mosca Branca
Feijão
Doença
Geminiviridae
Vigna unguiculata subsp. unguiculata var. spontanea
Bean golden mosaic virus
Begomovirus
description A new bipartite begomovirus (family Geminiviridae) was detected on cowpea (Vigna unguiculata) plants exhibiting bright golden mosaic symptoms on leaves under field conditions in Brazil. Complete consensus sequences of DNA-A and DNA-B components of an isolate of the virus (PE?088) were obtained by nanopore sequencing and confirmed by Sanger sequencing. The genome components presented the typical genomic organization of New World (NW) begomoviruses. Pairwise sequence comparisons revealed low levels of identity with other begomovirus species previously reported infecting cowpea around the world. Phylogenetic analysis using complete sequences of DNA-A components revealed that the closest relatives of PE?088 (85-87% nucleotide sequence identities) were three legume-infecting begomoviruses from Brazil: bean golden mosaic virus, macroptilium common mosaic virus and macroptilium yellow vein virus. According to the current classification criteria, PE?088 represents a new species in the genus Begomovirus, tentatively named as cowpea bright yellow mosaic virus (CoBYMV).
publishDate 2019
dc.date.none.fl_str_mv 2019
2020-02-13T18:16:37Z
2020-02-13T18:16:37Z
2020-02-13
2020-02-13T18:16:37Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Archives of Virology, v. 164, n. 7, p. 1907-1910, 2019.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120225
10.1007/s00705-019-04254-5
identifier_str_mv Archives of Virology, v. 164, n. 7, p. 1907-1910, 2019.
10.1007/s00705-019-04254-5
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120225
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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