Carotenoid biosynthesis strutural genes in carrot (Daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (SNP) markers and genome mapping.

• Autor(a) principal: JUST, B. J.
• Data de Publicação: 2007
• Outros Autores: SANTOS, C. A. F., FONSECA, M. E. N., BOITEUX, L. S., OLOIZIA, B. B., SIMON, P. W.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/780437
• topic: Mapa genômico; Sequência genética; cDNA; Carotenóide; Cenoura; Daucus Carota; Marcador Molecular; Polimorfismo

In this study, we utilized published carrot sequences as well as heterologous PCR approaches with primers derived from sequence information of other plant species to isolate 24 putative genes coding for carotenoid bio-synthesis enzymes in carrot. Twenty-two of these genes were placed on the carrot genetic linkage map developed from a cross between orange-rooted and white- rooted carrot. The carotenoid genes were distributed in eight of the nine linkage groups in the carrot genome recommending their use for merging maps. Two genes co-localized with a genomic region spanning one of the most significant quantitative trait loci (QTL) for carotenoid accumulation.