Use of "EP" (Peroxidase) allele in soybean varietal characterization.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1008802 |
Resumo: | The aim of this work was to evaluate the use of the molecular biology technique of PCR (Polimerase Chain Reaction) in the characterization of soybean cultivars. The study was performed at the Department of Plant Production, Faculty of Agricultural Sciences/ UNESP and Institute of Bioscience, Botucatu-SP. Fourteen commercial soybean cultivars were used, of which six were selected as positive reaction to peroxidase (BRS 320, BRS 284, BRS 232, BRS 7860RR, BRSMG 760SRR, BRS295RR), four as negative reaction (BRS 326, BRS 8160RR, BRSMG 800A (NutriSoy), BRS Valiosa RR) and four as double reaction (BRSGO 8060, BRS 270RR, FTS Campo Mourão and BRS 239). Thus, the results attained by the traditional biochemical colorimetric test for the 14 cultivars were compared with the conventional PCR assay. For PCR analysis, DNA was extracted from whole seeds and the primers were tested, and subsequently PCR and agarose gel electrophoresis were performed. The combination of primers prx9 + prx10 confirmed the use of the PCR reaction to characterize soybean cultivars considered doubtful by conventional colorimetric text. |
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Use of "EP" (Peroxidase) allele in soybean varietal characterization.Pureza genéticaEspécie agrícolaGenética MolecularGlycine MaxSojaThe aim of this work was to evaluate the use of the molecular biology technique of PCR (Polimerase Chain Reaction) in the characterization of soybean cultivars. The study was performed at the Department of Plant Production, Faculty of Agricultural Sciences/ UNESP and Institute of Bioscience, Botucatu-SP. Fourteen commercial soybean cultivars were used, of which six were selected as positive reaction to peroxidase (BRS 320, BRS 284, BRS 232, BRS 7860RR, BRSMG 760SRR, BRS295RR), four as negative reaction (BRS 326, BRS 8160RR, BRSMG 800A (NutriSoy), BRS Valiosa RR) and four as double reaction (BRSGO 8060, BRS 270RR, FTS Campo Mourão and BRS 239). Thus, the results attained by the traditional biochemical colorimetric test for the 14 cultivars were compared with the conventional PCR assay. For PCR analysis, DNA was extracted from whole seeds and the primers were tested, and subsequently PCR and agarose gel electrophoresis were performed. The combination of primers prx9 + prx10 confirmed the use of the PCR reaction to characterize soybean cultivars considered doubtful by conventional colorimetric text.Bárbara Panoff Valário, UNESP; Cláudio Cavariani, UNESP; JOSE DE BARROS FRANCA NETO, CNPSO; ELISA SERRA NEGRA VIEIRA, CNPF; Juliana Pereira Bravo, UNESP; Edvaldo Aparecido Amaral da Silva, UNESP.VALÁRIO, B. P.CAVARIANI, C.FRANÇA-NETO, J. B.VIEIRA, E. S. N.BRAVO, J. P.SILVA, E. A. A. da2015-02-13T11:11:11Z2015-02-13T11:11:11Z2015-02-1320142015-02-26T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Seed Science, v. 36, n. 4, p. 465-470, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1008802enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:03:09Zoai:www.alice.cnptia.embrapa.br:doc/1008802Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:03:09falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:03:09Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Use of "EP" (Peroxidase) allele in soybean varietal characterization. |
title |
Use of "EP" (Peroxidase) allele in soybean varietal characterization. |
spellingShingle |
Use of "EP" (Peroxidase) allele in soybean varietal characterization. VALÁRIO, B. P. Pureza genética Espécie agrícola Genética Molecular Glycine Max Soja |
title_short |
Use of "EP" (Peroxidase) allele in soybean varietal characterization. |
title_full |
Use of "EP" (Peroxidase) allele in soybean varietal characterization. |
title_fullStr |
Use of "EP" (Peroxidase) allele in soybean varietal characterization. |
title_full_unstemmed |
Use of "EP" (Peroxidase) allele in soybean varietal characterization. |
title_sort |
Use of "EP" (Peroxidase) allele in soybean varietal characterization. |
author |
VALÁRIO, B. P. |
author_facet |
VALÁRIO, B. P. CAVARIANI, C. FRANÇA-NETO, J. B. VIEIRA, E. S. N. BRAVO, J. P. SILVA, E. A. A. da |
author_role |
author |
author2 |
CAVARIANI, C. FRANÇA-NETO, J. B. VIEIRA, E. S. N. BRAVO, J. P. SILVA, E. A. A. da |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Bárbara Panoff Valário, UNESP; Cláudio Cavariani, UNESP; JOSE DE BARROS FRANCA NETO, CNPSO; ELISA SERRA NEGRA VIEIRA, CNPF; Juliana Pereira Bravo, UNESP; Edvaldo Aparecido Amaral da Silva, UNESP. |
dc.contributor.author.fl_str_mv |
VALÁRIO, B. P. CAVARIANI, C. FRANÇA-NETO, J. B. VIEIRA, E. S. N. BRAVO, J. P. SILVA, E. A. A. da |
dc.subject.por.fl_str_mv |
Pureza genética Espécie agrícola Genética Molecular Glycine Max Soja |
topic |
Pureza genética Espécie agrícola Genética Molecular Glycine Max Soja |
description |
The aim of this work was to evaluate the use of the molecular biology technique of PCR (Polimerase Chain Reaction) in the characterization of soybean cultivars. The study was performed at the Department of Plant Production, Faculty of Agricultural Sciences/ UNESP and Institute of Bioscience, Botucatu-SP. Fourteen commercial soybean cultivars were used, of which six were selected as positive reaction to peroxidase (BRS 320, BRS 284, BRS 232, BRS 7860RR, BRSMG 760SRR, BRS295RR), four as negative reaction (BRS 326, BRS 8160RR, BRSMG 800A (NutriSoy), BRS Valiosa RR) and four as double reaction (BRSGO 8060, BRS 270RR, FTS Campo Mourão and BRS 239). Thus, the results attained by the traditional biochemical colorimetric test for the 14 cultivars were compared with the conventional PCR assay. For PCR analysis, DNA was extracted from whole seeds and the primers were tested, and subsequently PCR and agarose gel electrophoresis were performed. The combination of primers prx9 + prx10 confirmed the use of the PCR reaction to characterize soybean cultivars considered doubtful by conventional colorimetric text. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-02-13T11:11:11Z 2015-02-13T11:11:11Z 2015-02-13 2015-02-26T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Seed Science, v. 36, n. 4, p. 465-470, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1008802 |
identifier_str_mv |
Journal of Seed Science, v. 36, n. 4, p. 465-470, 2014. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1008802 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503402480205824 |