Use of EP(Peroxidase) allele in soybean varietal characterization

• Autor(a) principal: Valário, Bárbara Panoff [UNESP]
• Data de Publicação: 2014
• Outros Autores: Cavariani, Cláudio [UNESP], França-neto, José De Barros, Vieira, Elisa Serra Negra, Bravo, Juliana Pereira [UNESP], Silva, Edvaldo Aparecido Amaral da [UNESP][
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/2317-1545v36n4996; http://hdl.handle.net/11449/114443
• topic: Glycine max (L.) Merrill; PCR; DNA; pureza genética; Glycine max (L.) Merrill; PCR; DNA; genetic purity

The aim of this work was to evaluate the use of the molecular biology technique of PCR (Polimerase Chain Reaction) in the characterization of soybean cultivars. The study was performed at the Department of Plant Production, Faculty of Agricultural Sciences/ UNESP and Institute of Bioscience, Botucatu-SP. Fourteen commercial soybean cultivars were used, of which six were selected as positive reaction to peroxidase (BRS 320, BRS 284, BRS 232, BRS 7860RR, BRSMG 760SRR, BRS295RR), four as negative reaction (BRS 326, BRS 8160RR, BRSMG 800A (NutriSoy), BRS Valiosa RR) and four as double reaction (BRSGO 8060, BRS 270RR, FTS Campo Mourão and BRS 239). Thus, the results attained by the traditional biochemical colorimetric test for the 14 cultivars were compared with the conventional PCR assay. For PCR analysis, DNA was extracted from whole seeds and the primers were tested, and subsequently PCR and agarose gel electrophoresis were performed. The combination of primers prx9 + prx10 confirmed the use of the PCR reaction to characterize soybean cultivars considered doubtful by conventional colorimetric text.