Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/898592 https://doi.org/10.1016/j.vetpar.2011.05.020 |
Resumo: | The most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coli, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (±0.73)% and 96.36 (±2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol® (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C. elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes. |
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Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use.Anthelmintic plantsC elegansgastrointestinal nematodesplant extractsThe most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coli, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (±0.73)% and 96.36 (±2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol® (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C. elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes.LUCIANA M. KATIKI, ISNTITUTO DE ZOOTECNIA/NOVA ODESSA; JORGE F. S. FERREIRA, Appalachian Farming Systems Research Center (USDA-ARS); ANNE M. ZAJAC, VIRGINIA-MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE, VIRGINIA TECH, BLACKSBURG; CAROL MASLER, NEMATOLOGY LABORATORY, USDA-ARS, BARC/ BELTSVILLE; DAVID S. LINDSAY, VIRGINIA-MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE, VIRGINIA TECH/ BLACKSBURG; ANA CAROLINA DE SOUZA CHAGAS, CPPSE; ALESSANDRO F. T. AMARANTE, UNESP/BOTUCATU.KATIKI, L. M.FERREIRA, J. F. S.ZAJAC, A. M.MASLER, C.LINDSAY, D. S.CHAGAS, A. C. de S.AMARANTE, A. F. T.2022-07-25T13:19:36Z2022-07-25T13:19:36Z2011-08-242011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVeterinary Parasitology, v. 182, n. 2-4, p. 264-268, dec. 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/898592https://doi.org/10.1016/j.vetpar.2011.05.020enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-07-25T13:19:44Zoai:www.alice.cnptia.embrapa.br:doc/898592Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-07-25T13:19:44falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-07-25T13:19:44Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. |
title |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. |
spellingShingle |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. KATIKI, L. M. Anthelmintic plants C elegans gastrointestinal nematodes plant extracts |
title_short |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. |
title_full |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. |
title_fullStr |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. |
title_full_unstemmed |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. |
title_sort |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. |
author |
KATIKI, L. M. |
author_facet |
KATIKI, L. M. FERREIRA, J. F. S. ZAJAC, A. M. MASLER, C. LINDSAY, D. S. CHAGAS, A. C. de S. AMARANTE, A. F. T. |
author_role |
author |
author2 |
FERREIRA, J. F. S. ZAJAC, A. M. MASLER, C. LINDSAY, D. S. CHAGAS, A. C. de S. AMARANTE, A. F. T. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
LUCIANA M. KATIKI, ISNTITUTO DE ZOOTECNIA/NOVA ODESSA; JORGE F. S. FERREIRA, Appalachian Farming Systems Research Center (USDA-ARS); ANNE M. ZAJAC, VIRGINIA-MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE, VIRGINIA TECH, BLACKSBURG; CAROL MASLER, NEMATOLOGY LABORATORY, USDA-ARS, BARC/ BELTSVILLE; DAVID S. LINDSAY, VIRGINIA-MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE, VIRGINIA TECH/ BLACKSBURG; ANA CAROLINA DE SOUZA CHAGAS, CPPSE; ALESSANDRO F. T. AMARANTE, UNESP/BOTUCATU. |
dc.contributor.author.fl_str_mv |
KATIKI, L. M. FERREIRA, J. F. S. ZAJAC, A. M. MASLER, C. LINDSAY, D. S. CHAGAS, A. C. de S. AMARANTE, A. F. T. |
dc.subject.por.fl_str_mv |
Anthelmintic plants C elegans gastrointestinal nematodes plant extracts |
topic |
Anthelmintic plants C elegans gastrointestinal nematodes plant extracts |
description |
The most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coli, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (±0.73)% and 96.36 (±2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol® (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C. elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-24 2011 2022-07-25T13:19:36Z 2022-07-25T13:19:36Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Veterinary Parasitology, v. 182, n. 2-4, p. 264-268, dec. 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/898592 https://doi.org/10.1016/j.vetpar.2011.05.020 |
identifier_str_mv |
Veterinary Parasitology, v. 182, n. 2-4, p. 264-268, dec. 2011. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/898592 https://doi.org/10.1016/j.vetpar.2011.05.020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503526690324480 |