Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use

Detalhes bibliográficos
Autor(a) principal: Katiki, Luciana M.
Data de Publicação: 2011
Outros Autores: Ferreira, Jorge F. S., Zajac, Anne M., Masler, Carol, Lindsay, David S., Chagas, Ana Carolina S., Amarante, Alessandro Francisco Talamini do [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vetpar.2011.05.020
http://hdl.handle.net/11449/18981
Resumo: The most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coil, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (+/- 0.73)% and 9636 (+/- 2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol (R) (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes. Published by Elsevier B.V.
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spelling Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary useC. elegansAnthelmintic plantsGastrointestinal nematodesPlant extractsThe most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coil, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (+/- 0.73)% and 9636 (+/- 2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol (R) (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes. Published by Elsevier B.V.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Appalachian Farming Systems Research Center (USDA-ARS)Virginia Polytechnic InstituteState University (Virginia Tech)USDA-ARS Nematology LabARS, Appalachian Farming Syst Res Ctr, USDA, Beaver, WV 25813 USAAPTA, Inst Zootecnia SAA, BR-13460000 Nova Odessa, SP, BrazilVirginia Tech, Virginia Maryland Reg Coll Vet Med, Blacksburg, VA 24060 USAARS, Nematol Lab, USDA, Beltsville, MD 20705 USAEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Pecuaria Sudeste, São Carlos, SP, BrazilUNESP Univ Estadual Paulista, Dept Parasitol IB, Botucatu, SP, BrazilUNESP Univ Estadual Paulista, Dept Parasitol IB, Botucatu, SP, BrazilElsevier B.V.ARSAgência Paulista de Tecnologia dos Agronegócios (APTA)Virginia TechEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Katiki, Luciana M.Ferreira, Jorge F. S.Zajac, Anne M.Masler, CarolLindsay, David S.Chagas, Ana Carolina S.Amarante, Alessandro Francisco Talamini do [UNESP]2014-05-20T13:53:13Z2014-05-20T13:53:13Z2011-12-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article264-268application/pdfhttp://dx.doi.org/10.1016/j.vetpar.2011.05.020Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 182, n. 2-4, p. 264-268, 2011.0304-4017http://hdl.handle.net/11449/1898110.1016/j.vetpar.2011.05.020WOS:000297524900017WOS000297524900017.pdf2677231663329706Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology2.4221,275info:eu-repo/semantics/openAccess2024-01-25T06:29:03Zoai:repositorio.unesp.br:11449/18981Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:54:08.916076Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use
title Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use
spellingShingle Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use
Katiki, Luciana M.
C. elegans
Anthelmintic plants
Gastrointestinal nematodes
Plant extracts
title_short Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use
title_full Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use
title_fullStr Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use
title_full_unstemmed Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use
title_sort Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use
author Katiki, Luciana M.
author_facet Katiki, Luciana M.
Ferreira, Jorge F. S.
Zajac, Anne M.
Masler, Carol
Lindsay, David S.
Chagas, Ana Carolina S.
Amarante, Alessandro Francisco Talamini do [UNESP]
author_role author
author2 Ferreira, Jorge F. S.
Zajac, Anne M.
Masler, Carol
Lindsay, David S.
Chagas, Ana Carolina S.
Amarante, Alessandro Francisco Talamini do [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv ARS
Agência Paulista de Tecnologia dos Agronegócios (APTA)
Virginia Tech
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Katiki, Luciana M.
Ferreira, Jorge F. S.
Zajac, Anne M.
Masler, Carol
Lindsay, David S.
Chagas, Ana Carolina S.
Amarante, Alessandro Francisco Talamini do [UNESP]
dc.subject.por.fl_str_mv C. elegans
Anthelmintic plants
Gastrointestinal nematodes
Plant extracts
topic C. elegans
Anthelmintic plants
Gastrointestinal nematodes
Plant extracts
description The most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coil, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (+/- 0.73)% and 9636 (+/- 2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol (R) (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes. Published by Elsevier B.V.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-15
2014-05-20T13:53:13Z
2014-05-20T13:53:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vetpar.2011.05.020
Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 182, n. 2-4, p. 264-268, 2011.
0304-4017
http://hdl.handle.net/11449/18981
10.1016/j.vetpar.2011.05.020
WOS:000297524900017
WOS000297524900017.pdf
2677231663329706
url http://dx.doi.org/10.1016/j.vetpar.2011.05.020
http://hdl.handle.net/11449/18981
identifier_str_mv Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 182, n. 2-4, p. 264-268, 2011.
0304-4017
10.1016/j.vetpar.2011.05.020
WOS:000297524900017
WOS000297524900017.pdf
2677231663329706
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Parasitology
2.422
1,275
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 264-268
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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