Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetpar.2011.05.020 http://hdl.handle.net/11449/18981 |
Resumo: | The most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coil, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (+/- 0.73)% and 9636 (+/- 2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol (R) (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes. Published by Elsevier B.V. |
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Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary useC. elegansAnthelmintic plantsGastrointestinal nematodesPlant extractsThe most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coil, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (+/- 0.73)% and 9636 (+/- 2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol (R) (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes. Published by Elsevier B.V.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Appalachian Farming Systems Research Center (USDA-ARS)Virginia Polytechnic InstituteState University (Virginia Tech)USDA-ARS Nematology LabARS, Appalachian Farming Syst Res Ctr, USDA, Beaver, WV 25813 USAAPTA, Inst Zootecnia SAA, BR-13460000 Nova Odessa, SP, BrazilVirginia Tech, Virginia Maryland Reg Coll Vet Med, Blacksburg, VA 24060 USAARS, Nematol Lab, USDA, Beltsville, MD 20705 USAEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Pecuaria Sudeste, São Carlos, SP, BrazilUNESP Univ Estadual Paulista, Dept Parasitol IB, Botucatu, SP, BrazilUNESP Univ Estadual Paulista, Dept Parasitol IB, Botucatu, SP, BrazilElsevier B.V.ARSAgência Paulista de Tecnologia dos Agronegócios (APTA)Virginia TechEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Katiki, Luciana M.Ferreira, Jorge F. S.Zajac, Anne M.Masler, CarolLindsay, David S.Chagas, Ana Carolina S.Amarante, Alessandro Francisco Talamini do [UNESP]2014-05-20T13:53:13Z2014-05-20T13:53:13Z2011-12-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article264-268application/pdfhttp://dx.doi.org/10.1016/j.vetpar.2011.05.020Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 182, n. 2-4, p. 264-268, 2011.0304-4017http://hdl.handle.net/11449/1898110.1016/j.vetpar.2011.05.020WOS:000297524900017WOS000297524900017.pdf2677231663329706Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology2.4221,275info:eu-repo/semantics/openAccess2024-01-25T06:29:03Zoai:repositorio.unesp.br:11449/18981Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:54:08.916076Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use |
title |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use |
spellingShingle |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use Katiki, Luciana M. C. elegans Anthelmintic plants Gastrointestinal nematodes Plant extracts |
title_short |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use |
title_full |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use |
title_fullStr |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use |
title_full_unstemmed |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use |
title_sort |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use |
author |
Katiki, Luciana M. |
author_facet |
Katiki, Luciana M. Ferreira, Jorge F. S. Zajac, Anne M. Masler, Carol Lindsay, David S. Chagas, Ana Carolina S. Amarante, Alessandro Francisco Talamini do [UNESP] |
author_role |
author |
author2 |
Ferreira, Jorge F. S. Zajac, Anne M. Masler, Carol Lindsay, David S. Chagas, Ana Carolina S. Amarante, Alessandro Francisco Talamini do [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
ARS Agência Paulista de Tecnologia dos Agronegócios (APTA) Virginia Tech Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Katiki, Luciana M. Ferreira, Jorge F. S. Zajac, Anne M. Masler, Carol Lindsay, David S. Chagas, Ana Carolina S. Amarante, Alessandro Francisco Talamini do [UNESP] |
dc.subject.por.fl_str_mv |
C. elegans Anthelmintic plants Gastrointestinal nematodes Plant extracts |
topic |
C. elegans Anthelmintic plants Gastrointestinal nematodes Plant extracts |
description |
The most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coil, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (+/- 0.73)% and 9636 (+/- 2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol (R) (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes. Published by Elsevier B.V. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-15 2014-05-20T13:53:13Z 2014-05-20T13:53:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetpar.2011.05.020 Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 182, n. 2-4, p. 264-268, 2011. 0304-4017 http://hdl.handle.net/11449/18981 10.1016/j.vetpar.2011.05.020 WOS:000297524900017 WOS000297524900017.pdf 2677231663329706 |
url |
http://dx.doi.org/10.1016/j.vetpar.2011.05.020 http://hdl.handle.net/11449/18981 |
identifier_str_mv |
Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 182, n. 2-4, p. 264-268, 2011. 0304-4017 10.1016/j.vetpar.2011.05.020 WOS:000297524900017 WOS000297524900017.pdf 2677231663329706 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Parasitology 2.422 1,275 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
264-268 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129562940276736 |