Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134629 http://dx.doi.org/10.21577/0103-5053.20170089 |
Resumo: | Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+ , 77Se+ , 78Se+ and 82Se+ without using CRI and 82Se+ with CRI. |
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Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation.Bovine semenCattle fertilityseleniumDetermination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+ , 77Se+ , 78Se+ and 82Se+ without using CRI and 82Se+ with CRI.SILMARA ROSSANA BIANCHI, CNPS; Clarice Dias Britto do Amaral, UFPR; Caroline Santos da Silva, UFSCar; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.BIANCHI, S. R.AMARAL, C. D. B. doSILVA, C. S. daNOGUEIRA, A. R. de A.2021-09-21T18:00:55Z2021-09-21T18:00:55Z2021-09-212017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of the Brazilian Chemical Society, v. 28, n. 12, p. 2357-2364, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134629http://dx.doi.org/10.21577/0103-5053.20170089enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-09-21T18:01:04Zoai:www.alice.cnptia.embrapa.br:doc/1134629Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-09-21T18:01:04falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-09-21T18:01:04Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation. |
title |
Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation. |
spellingShingle |
Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation. BIANCHI, S. R. Bovine semen Cattle fertility selenium |
title_short |
Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation. |
title_full |
Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation. |
title_fullStr |
Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation. |
title_full_unstemmed |
Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation. |
title_sort |
Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation. |
author |
BIANCHI, S. R. |
author_facet |
BIANCHI, S. R. AMARAL, C. D. B. do SILVA, C. S. da NOGUEIRA, A. R. de A. |
author_role |
author |
author2 |
AMARAL, C. D. B. do SILVA, C. S. da NOGUEIRA, A. R. de A. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
SILMARA ROSSANA BIANCHI, CNPS; Clarice Dias Britto do Amaral, UFPR; Caroline Santos da Silva, UFSCar; ANA RITA DE ARAUJO NOGUEIRA, CPPSE. |
dc.contributor.author.fl_str_mv |
BIANCHI, S. R. AMARAL, C. D. B. do SILVA, C. S. da NOGUEIRA, A. R. de A. |
dc.subject.por.fl_str_mv |
Bovine semen Cattle fertility selenium |
topic |
Bovine semen Cattle fertility selenium |
description |
Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+ , 77Se+ , 78Se+ and 82Se+ without using CRI and 82Se+ with CRI. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2021-09-21T18:00:55Z 2021-09-21T18:00:55Z 2021-09-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of the Brazilian Chemical Society, v. 28, n. 12, p. 2357-2364, 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134629 http://dx.doi.org/10.21577/0103-5053.20170089 |
identifier_str_mv |
Journal of the Brazilian Chemical Society, v. 28, n. 12, p. 2357-2364, 2017. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134629 http://dx.doi.org/10.21577/0103-5053.20170089 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503509790425088 |