Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation

Detalhes bibliográficos
Autor(a) principal: Bianchi,Silmara R.
Data de Publicação: 2017
Outros Autores: Amaral,Clarice D. B., Silva,Caroline S., Nogueira,Ana Rita A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017001202357
Resumo: Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+, 77Se+, 78Se+ and 82Se+ without using CRI and 82Se+ with CRI.
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spelling Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample PreparationseleniumICP-MSbovine semendiluted formic acidcattle fertilityDetermination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+, 77Se+, 78Se+ and 82Se+ without using CRI and 82Se+ with CRI.Sociedade Brasileira de Química2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017001202357Journal of the Brazilian Chemical Society v.28 n.12 2017reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20170089info:eu-repo/semantics/openAccessBianchi,Silmara R.Amaral,Clarice D. B.Silva,Caroline S.Nogueira,Ana Rita A.eng2017-11-06T00:00:00Zoai:scielo:S0103-50532017001202357Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-11-06T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation
title Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation
spellingShingle Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation
Bianchi,Silmara R.
selenium
ICP-MS
bovine semen
diluted formic acid
cattle fertility
title_short Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation
title_full Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation
title_fullStr Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation
title_full_unstemmed Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation
title_sort Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation
author Bianchi,Silmara R.
author_facet Bianchi,Silmara R.
Amaral,Clarice D. B.
Silva,Caroline S.
Nogueira,Ana Rita A.
author_role author
author2 Amaral,Clarice D. B.
Silva,Caroline S.
Nogueira,Ana Rita A.
author2_role author
author
author
dc.contributor.author.fl_str_mv Bianchi,Silmara R.
Amaral,Clarice D. B.
Silva,Caroline S.
Nogueira,Ana Rita A.
dc.subject.por.fl_str_mv selenium
ICP-MS
bovine semen
diluted formic acid
cattle fertility
topic selenium
ICP-MS
bovine semen
diluted formic acid
cattle fertility
description Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+, 77Se+, 78Se+ and 82Se+ without using CRI and 82Se+ with CRI.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017001202357
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017001202357
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.21577/0103-5053.20170089
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.28 n.12 2017
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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