Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017001202357 |
Resumo: | Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+, 77Se+, 78Se+ and 82Se+ without using CRI and 82Se+ with CRI. |
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Journal of the Brazilian Chemical Society (Online) |
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Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample PreparationseleniumICP-MSbovine semendiluted formic acidcattle fertilityDetermination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+, 77Se+, 78Se+ and 82Se+ without using CRI and 82Se+ with CRI.Sociedade Brasileira de Química2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017001202357Journal of the Brazilian Chemical Society v.28 n.12 2017reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20170089info:eu-repo/semantics/openAccessBianchi,Silmara R.Amaral,Clarice D. B.Silva,Caroline S.Nogueira,Ana Rita A.eng2017-11-06T00:00:00Zoai:scielo:S0103-50532017001202357Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-11-06T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation |
title |
Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation |
spellingShingle |
Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation Bianchi,Silmara R. selenium ICP-MS bovine semen diluted formic acid cattle fertility |
title_short |
Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation |
title_full |
Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation |
title_fullStr |
Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation |
title_full_unstemmed |
Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation |
title_sort |
Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation |
author |
Bianchi,Silmara R. |
author_facet |
Bianchi,Silmara R. Amaral,Clarice D. B. Silva,Caroline S. Nogueira,Ana Rita A. |
author_role |
author |
author2 |
Amaral,Clarice D. B. Silva,Caroline S. Nogueira,Ana Rita A. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Bianchi,Silmara R. Amaral,Clarice D. B. Silva,Caroline S. Nogueira,Ana Rita A. |
dc.subject.por.fl_str_mv |
selenium ICP-MS bovine semen diluted formic acid cattle fertility |
topic |
selenium ICP-MS bovine semen diluted formic acid cattle fertility |
description |
Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+, 77Se+, 78Se+ and 82Se+ without using CRI and 82Se+ with CRI. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017001202357 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017001202357 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20170089 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.28 n.12 2017 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318180055646208 |