TH1 and TH2 cytokines mRNA levels in Brazilian Somalis crossbreed sheep resistant and suceptible to Trichostrongylus colubriformis infection.

Detalhes bibliográficos
Autor(a) principal: ZAROS, L. G.
Data de Publicação: 2010
Outros Autores: COUTINHO, L. L., NAVARRO, A. M. C., NEVES, M. R. N., BENVENUTI, C. L., SIDER, L. H., VIEIRA, L. da S.
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/867945
Resumo: Cytokines are proteins that play a central role in immune mechanisms involved in defense against gastrointestinal nematodes infections. The present study used the real-time PCR methodology to quantify Brazilian Somalis crossbreed sheep cytokines (IL-4, IL-13, TNF-? and IFN-?) in two groups: one resistant and other susceptible to Trichostrongylus colubriformis infection. From a Somalis sheep herd, 75 young animals were kept together on pasture without anthelmintic treatment for 4 months. The eight most resistant and the eight most susceptible animals were chosen based on the mean of fecal egg counts and slaughtered for recover the parasites and small intestine tissue samples collection. RT-PCR was performed using the LightCycler PCR and SYBR Green I dye. SDHA (succinate dehydrogenase complex subunit A) was used for normalization and the relative quantifi cation of genes was calculated by REST software. Resistant animals presented lower EPG counts than susceptible animals (1312,5 and 5081,6, respectively; P<0.0001) and 3 fold less specimens of Trichostrongylus colubriformis (P<0.05). Only IL-13 was up-regulated in resistant animals (P<0.02) and the other three genes analyzed, IL-4, TNF-? and IFN-? were down-regulated in this group, although not signifi cantly (P>0.05). IL-13 is a cytokine that stimulates the TH2 response, leading the host to quickly and effi ciently respond to the infection and contributes to the parasite expulsion. Although IL-4 acts together with IL-13 in this process, IL-13 is independent to eliminate the parasite for itself. It can be inferred that in the resistant animals a bias of TH2 type response was activated.
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spelling TH1 and TH2 cytokines mRNA levels in Brazilian Somalis crossbreed sheep resistant and suceptible to Trichostrongylus colubriformis infection.Raça SomalisParasito animalNematódeo gastrintestinalCitocinaOvinoInfecçãoResistênciaTrichostrongylus colubriformisSheep diseasesNematode infectionsCytokines are proteins that play a central role in immune mechanisms involved in defense against gastrointestinal nematodes infections. The present study used the real-time PCR methodology to quantify Brazilian Somalis crossbreed sheep cytokines (IL-4, IL-13, TNF-? and IFN-?) in two groups: one resistant and other susceptible to Trichostrongylus colubriformis infection. From a Somalis sheep herd, 75 young animals were kept together on pasture without anthelmintic treatment for 4 months. The eight most resistant and the eight most susceptible animals were chosen based on the mean of fecal egg counts and slaughtered for recover the parasites and small intestine tissue samples collection. RT-PCR was performed using the LightCycler PCR and SYBR Green I dye. SDHA (succinate dehydrogenase complex subunit A) was used for normalization and the relative quantifi cation of genes was calculated by REST software. Resistant animals presented lower EPG counts than susceptible animals (1312,5 and 5081,6, respectively; P<0.0001) and 3 fold less specimens of Trichostrongylus colubriformis (P<0.05). Only IL-13 was up-regulated in resistant animals (P<0.02) and the other three genes analyzed, IL-4, TNF-? and IFN-? were down-regulated in this group, although not signifi cantly (P>0.05). IL-13 is a cytokine that stimulates the TH2 response, leading the host to quickly and effi ciently respond to the infection and contributes to the parasite expulsion. Although IL-4 acts together with IL-13 in this process, IL-13 is independent to eliminate the parasite for itself. It can be inferred that in the resistant animals a bias of TH2 type response was activated.Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN.; ESALQ, USP, Piracicaba, SP; Universidade Estadual Vale do Acaraú (UVA), Sobral, CE; UVA; UVA; LUCIA HELENA SIDER, CNPC; LUIZ DA SILVA VIEIRA, CNPC.ZAROS, L. G.COUTINHO, L. L.NAVARRO, A. M. C.NEVES, M. R. N.BENVENUTI, C. L.SIDER, L. H.VIEIRA, L. da S.2011-10-18T00:04:54Z2011-10-18T00:04:54Z2010-11-2520102019-09-24T11:11:11ZResumo em anais e proceedingsinfo:eu-repo/semantics/publishedVersionIn: CONFERENCE FOR THE INTERNATIONAL SOCIETY FOR ANIMAL GENETICS, 32., 2010. Edinburgo (UK). Proceedings... Edinburgo: ISAG, 2010. p. 132. Abstract P5052.http://www.alice.cnptia.embrapa.br/alice/handle/doc/867945enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:45:43Zoai:www.alice.cnptia.embrapa.br:doc/867945Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:45:43Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv TH1 and TH2 cytokines mRNA levels in Brazilian Somalis crossbreed sheep resistant and suceptible to Trichostrongylus colubriformis infection.
title TH1 and TH2 cytokines mRNA levels in Brazilian Somalis crossbreed sheep resistant and suceptible to Trichostrongylus colubriformis infection.
spellingShingle TH1 and TH2 cytokines mRNA levels in Brazilian Somalis crossbreed sheep resistant and suceptible to Trichostrongylus colubriformis infection.
ZAROS, L. G.
Raça Somalis
Parasito animal
Nematódeo gastrintestinal
Citocina
Ovino
Infecção
Resistência
Trichostrongylus colubriformis
Sheep diseases
Nematode infections
title_short TH1 and TH2 cytokines mRNA levels in Brazilian Somalis crossbreed sheep resistant and suceptible to Trichostrongylus colubriformis infection.
title_full TH1 and TH2 cytokines mRNA levels in Brazilian Somalis crossbreed sheep resistant and suceptible to Trichostrongylus colubriformis infection.
title_fullStr TH1 and TH2 cytokines mRNA levels in Brazilian Somalis crossbreed sheep resistant and suceptible to Trichostrongylus colubriformis infection.
title_full_unstemmed TH1 and TH2 cytokines mRNA levels in Brazilian Somalis crossbreed sheep resistant and suceptible to Trichostrongylus colubriformis infection.
title_sort TH1 and TH2 cytokines mRNA levels in Brazilian Somalis crossbreed sheep resistant and suceptible to Trichostrongylus colubriformis infection.
author ZAROS, L. G.
author_facet ZAROS, L. G.
COUTINHO, L. L.
NAVARRO, A. M. C.
NEVES, M. R. N.
BENVENUTI, C. L.
SIDER, L. H.
VIEIRA, L. da S.
author_role author
author2 COUTINHO, L. L.
NAVARRO, A. M. C.
NEVES, M. R. N.
BENVENUTI, C. L.
SIDER, L. H.
VIEIRA, L. da S.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN.; ESALQ, USP, Piracicaba, SP; Universidade Estadual Vale do Acaraú (UVA), Sobral, CE; UVA; UVA; LUCIA HELENA SIDER, CNPC; LUIZ DA SILVA VIEIRA, CNPC.
dc.contributor.author.fl_str_mv ZAROS, L. G.
COUTINHO, L. L.
NAVARRO, A. M. C.
NEVES, M. R. N.
BENVENUTI, C. L.
SIDER, L. H.
VIEIRA, L. da S.
dc.subject.por.fl_str_mv Raça Somalis
Parasito animal
Nematódeo gastrintestinal
Citocina
Ovino
Infecção
Resistência
Trichostrongylus colubriformis
Sheep diseases
Nematode infections
topic Raça Somalis
Parasito animal
Nematódeo gastrintestinal
Citocina
Ovino
Infecção
Resistência
Trichostrongylus colubriformis
Sheep diseases
Nematode infections
description Cytokines are proteins that play a central role in immune mechanisms involved in defense against gastrointestinal nematodes infections. The present study used the real-time PCR methodology to quantify Brazilian Somalis crossbreed sheep cytokines (IL-4, IL-13, TNF-? and IFN-?) in two groups: one resistant and other susceptible to Trichostrongylus colubriformis infection. From a Somalis sheep herd, 75 young animals were kept together on pasture without anthelmintic treatment for 4 months. The eight most resistant and the eight most susceptible animals were chosen based on the mean of fecal egg counts and slaughtered for recover the parasites and small intestine tissue samples collection. RT-PCR was performed using the LightCycler PCR and SYBR Green I dye. SDHA (succinate dehydrogenase complex subunit A) was used for normalization and the relative quantifi cation of genes was calculated by REST software. Resistant animals presented lower EPG counts than susceptible animals (1312,5 and 5081,6, respectively; P<0.0001) and 3 fold less specimens of Trichostrongylus colubriformis (P<0.05). Only IL-13 was up-regulated in resistant animals (P<0.02) and the other three genes analyzed, IL-4, TNF-? and IFN-? were down-regulated in this group, although not signifi cantly (P>0.05). IL-13 is a cytokine that stimulates the TH2 response, leading the host to quickly and effi ciently respond to the infection and contributes to the parasite expulsion. Although IL-4 acts together with IL-13 in this process, IL-13 is independent to eliminate the parasite for itself. It can be inferred that in the resistant animals a bias of TH2 type response was activated.
publishDate 2010
dc.date.none.fl_str_mv 2010-11-25
2010
2011-10-18T00:04:54Z
2011-10-18T00:04:54Z
2019-09-24T11:11:11Z
dc.type.driver.fl_str_mv Resumo em anais e proceedings
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv In: CONFERENCE FOR THE INTERNATIONAL SOCIETY FOR ANIMAL GENETICS, 32., 2010. Edinburgo (UK). Proceedings... Edinburgo: ISAG, 2010. p. 132. Abstract P5052.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/867945
identifier_str_mv In: CONFERENCE FOR THE INTERNATIONAL SOCIETY FOR ANIMAL GENETICS, 32., 2010. Edinburgo (UK). Proceedings... Edinburgo: ISAG, 2010. p. 132. Abstract P5052.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/867945
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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