Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162855 |
Resumo: | Plants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphere and endosphere microbiomes of a foxsusceptible and fox-resistant common bean cultivar. Integration of 16S-amplicon, shotgun metagenome as well as metatranscriptome sequencing with community ecology analysis showed that fox infections significantly changed the composition and gene expression of the root microbiome in a cultivar-dependent manner. More specifically, fox infection led to increased microbial diversity, network complexity, and a higher proportion of the genera Flavobacterium, Bacillus, and Dyadobacter in the rhizosphere of the fox-resistant cultivar compared to the fox-susceptible cultivar. In the endosphere, root infection also led to changes in community assembly, with a higher abundance of the genera Sinorhizobium and Ensifer in the fox-resistant cultivar. Metagenome and metatranscriptome analyses further revealed the enrichment of terpene biosynthesis genes with a potential role in pathogen suppression in the fox-resistant cultivar upon fungal pathogen invasion. Collectively, these results revealed a cultivar-dependent enrichment of specific bacterial genera and the activation of putative disease-suppressive functions in the rhizosphere and endosphere microbiome of common bean under siege. |
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Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.EndosphereMetagenomeMetatranscriptomePlant-microbe interactionRizosferaRhizospherePlants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphere and endosphere microbiomes of a foxsusceptible and fox-resistant common bean cultivar. Integration of 16S-amplicon, shotgun metagenome as well as metatranscriptome sequencing with community ecology analysis showed that fox infections significantly changed the composition and gene expression of the root microbiome in a cultivar-dependent manner. More specifically, fox infection led to increased microbial diversity, network complexity, and a higher proportion of the genera Flavobacterium, Bacillus, and Dyadobacter in the rhizosphere of the fox-resistant cultivar compared to the fox-susceptible cultivar. In the endosphere, root infection also led to changes in community assembly, with a higher abundance of the genera Sinorhizobium and Ensifer in the fox-resistant cultivar. Metagenome and metatranscriptome analyses further revealed the enrichment of terpene biosynthesis genes with a potential role in pathogen suppression in the fox-resistant cultivar upon fungal pathogen invasion. Collectively, these results revealed a cultivar-dependent enrichment of specific bacterial genera and the activation of putative disease-suppressive functions in the rhizosphere and endosphere microbiome of common bean under siege.LUCAS WILLIAM MENDES, CENA/USP; JOS M. RAAIJMAKERS, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; MATTIAS DE HOLLANDER, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; EDIS SEPO, LEIDEN UNIVERSITY, THE NETHERLANDS; RUTH GÓMES EXPÓSITO, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; RODRIGO MENDES, CNPMA; SIU MUI TSAI, CENA/USP; VICTOR J. CARRÓN, CENA/USP.MENDES, L. W.RAAIJMAKERS, J. M.HOLLANDER, M. DESEPO, E.EXPÓSITO, R. G.MENDES, R.TSAI, S. M.CARRÓN, V. J.2024-03-15T13:57:43Z2024-03-15T13:57:43Z2024-03-152023Resumo em anais e proceedingsinfo:eu-repo/semantics/publishedVersion1 p.In: PLANT MICROBIOME SYMPOSIUM, 4., 2023, Quito. Abstracts... Quito, Equador: Universidad San Francisco de Quito, 2023. Ref. 2-C.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162855porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-03-15T13:57:43Zoai:www.alice.cnptia.embrapa.br:doc/1162855Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-03-15T13:57:43Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome. |
title |
Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome. |
spellingShingle |
Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome. MENDES, L. W. Endosphere Metagenome Metatranscriptome Plant-microbe interaction Rizosfera Rhizosphere |
title_short |
Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome. |
title_full |
Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome. |
title_fullStr |
Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome. |
title_full_unstemmed |
Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome. |
title_sort |
Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome. |
author |
MENDES, L. W. |
author_facet |
MENDES, L. W. RAAIJMAKERS, J. M. HOLLANDER, M. DE SEPO, E. EXPÓSITO, R. G. MENDES, R. TSAI, S. M. CARRÓN, V. J. |
author_role |
author |
author2 |
RAAIJMAKERS, J. M. HOLLANDER, M. DE SEPO, E. EXPÓSITO, R. G. MENDES, R. TSAI, S. M. CARRÓN, V. J. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
LUCAS WILLIAM MENDES, CENA/USP; JOS M. RAAIJMAKERS, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; MATTIAS DE HOLLANDER, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; EDIS SEPO, LEIDEN UNIVERSITY, THE NETHERLANDS; RUTH GÓMES EXPÓSITO, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; RODRIGO MENDES, CNPMA; SIU MUI TSAI, CENA/USP; VICTOR J. CARRÓN, CENA/USP. |
dc.contributor.author.fl_str_mv |
MENDES, L. W. RAAIJMAKERS, J. M. HOLLANDER, M. DE SEPO, E. EXPÓSITO, R. G. MENDES, R. TSAI, S. M. CARRÓN, V. J. |
dc.subject.por.fl_str_mv |
Endosphere Metagenome Metatranscriptome Plant-microbe interaction Rizosfera Rhizosphere |
topic |
Endosphere Metagenome Metatranscriptome Plant-microbe interaction Rizosfera Rhizosphere |
description |
Plants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphere and endosphere microbiomes of a foxsusceptible and fox-resistant common bean cultivar. Integration of 16S-amplicon, shotgun metagenome as well as metatranscriptome sequencing with community ecology analysis showed that fox infections significantly changed the composition and gene expression of the root microbiome in a cultivar-dependent manner. More specifically, fox infection led to increased microbial diversity, network complexity, and a higher proportion of the genera Flavobacterium, Bacillus, and Dyadobacter in the rhizosphere of the fox-resistant cultivar compared to the fox-susceptible cultivar. In the endosphere, root infection also led to changes in community assembly, with a higher abundance of the genera Sinorhizobium and Ensifer in the fox-resistant cultivar. Metagenome and metatranscriptome analyses further revealed the enrichment of terpene biosynthesis genes with a potential role in pathogen suppression in the fox-resistant cultivar upon fungal pathogen invasion. Collectively, these results revealed a cultivar-dependent enrichment of specific bacterial genera and the activation of putative disease-suppressive functions in the rhizosphere and endosphere microbiome of common bean under siege. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 2024-03-15T13:57:43Z 2024-03-15T13:57:43Z 2024-03-15 |
dc.type.driver.fl_str_mv |
Resumo em anais e proceedings |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
In: PLANT MICROBIOME SYMPOSIUM, 4., 2023, Quito. Abstracts... Quito, Equador: Universidad San Francisco de Quito, 2023. Ref. 2-C. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162855 |
identifier_str_mv |
In: PLANT MICROBIOME SYMPOSIUM, 4., 2023, Quito. Abstracts... Quito, Equador: Universidad San Francisco de Quito, 2023. Ref. 2-C. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162855 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1 p. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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