Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.

Detalhes bibliográficos
Autor(a) principal: MENDES, L. W.
Data de Publicação: 2023
Outros Autores: RAAIJMAKERS, J. M., HOLLANDER, M. DE, SEPO, E., EXPÓSITO, R. G., MENDES, R., TSAI, S. M., CARRÓN, V. J.
Idioma: por
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162855
Resumo: Plants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphere and endosphere microbiomes of a foxsusceptible and fox-resistant common bean cultivar. Integration of 16S-amplicon, shotgun metagenome as well as metatranscriptome sequencing with community ecology analysis showed that fox infections significantly changed the composition and gene expression of the root microbiome in a cultivar-dependent manner. More specifically, fox infection led to increased microbial diversity, network complexity, and a higher proportion of the genera Flavobacterium, Bacillus, and Dyadobacter in the rhizosphere of the fox-resistant cultivar compared to the fox-susceptible cultivar. In the endosphere, root infection also led to changes in community assembly, with a higher abundance of the genera Sinorhizobium and Ensifer in the fox-resistant cultivar. Metagenome and metatranscriptome analyses further revealed the enrichment of terpene biosynthesis genes with a potential role in pathogen suppression in the fox-resistant cultivar upon fungal pathogen invasion. Collectively, these results revealed a cultivar-dependent enrichment of specific bacterial genera and the activation of putative disease-suppressive functions in the rhizosphere and endosphere microbiome of common bean under siege.
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spelling Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.EndosphereMetagenomeMetatranscriptomePlant-microbe interactionRizosferaRhizospherePlants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphere and endosphere microbiomes of a foxsusceptible and fox-resistant common bean cultivar. Integration of 16S-amplicon, shotgun metagenome as well as metatranscriptome sequencing with community ecology analysis showed that fox infections significantly changed the composition and gene expression of the root microbiome in a cultivar-dependent manner. More specifically, fox infection led to increased microbial diversity, network complexity, and a higher proportion of the genera Flavobacterium, Bacillus, and Dyadobacter in the rhizosphere of the fox-resistant cultivar compared to the fox-susceptible cultivar. In the endosphere, root infection also led to changes in community assembly, with a higher abundance of the genera Sinorhizobium and Ensifer in the fox-resistant cultivar. Metagenome and metatranscriptome analyses further revealed the enrichment of terpene biosynthesis genes with a potential role in pathogen suppression in the fox-resistant cultivar upon fungal pathogen invasion. Collectively, these results revealed a cultivar-dependent enrichment of specific bacterial genera and the activation of putative disease-suppressive functions in the rhizosphere and endosphere microbiome of common bean under siege.LUCAS WILLIAM MENDES, CENA/USP; JOS M. RAAIJMAKERS, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; MATTIAS DE HOLLANDER, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; EDIS SEPO, LEIDEN UNIVERSITY, THE NETHERLANDS; RUTH GÓMES EXPÓSITO, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; RODRIGO MENDES, CNPMA; SIU MUI TSAI, CENA/USP; VICTOR J. CARRÓN, CENA/USP.MENDES, L. W.RAAIJMAKERS, J. M.HOLLANDER, M. DESEPO, E.EXPÓSITO, R. G.MENDES, R.TSAI, S. M.CARRÓN, V. J.2024-03-15T13:57:43Z2024-03-15T13:57:43Z2024-03-152023Resumo em anais e proceedingsinfo:eu-repo/semantics/publishedVersion1 p.In: PLANT MICROBIOME SYMPOSIUM, 4., 2023, Quito. Abstracts... Quito, Equador: Universidad San Francisco de Quito, 2023. Ref. 2-C.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162855porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-03-15T13:57:43Zoai:www.alice.cnptia.embrapa.br:doc/1162855Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-03-15T13:57:43Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.
title Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.
spellingShingle Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.
MENDES, L. W.
Endosphere
Metagenome
Metatranscriptome
Plant-microbe interaction
Rizosfera
Rhizosphere
title_short Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.
title_full Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.
title_fullStr Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.
title_full_unstemmed Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.
title_sort Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.
author MENDES, L. W.
author_facet MENDES, L. W.
RAAIJMAKERS, J. M.
HOLLANDER, M. DE
SEPO, E.
EXPÓSITO, R. G.
MENDES, R.
TSAI, S. M.
CARRÓN, V. J.
author_role author
author2 RAAIJMAKERS, J. M.
HOLLANDER, M. DE
SEPO, E.
EXPÓSITO, R. G.
MENDES, R.
TSAI, S. M.
CARRÓN, V. J.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LUCAS WILLIAM MENDES, CENA/USP; JOS M. RAAIJMAKERS, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; MATTIAS DE HOLLANDER, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; EDIS SEPO, LEIDEN UNIVERSITY, THE NETHERLANDS; RUTH GÓMES EXPÓSITO, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; RODRIGO MENDES, CNPMA; SIU MUI TSAI, CENA/USP; VICTOR J. CARRÓN, CENA/USP.
dc.contributor.author.fl_str_mv MENDES, L. W.
RAAIJMAKERS, J. M.
HOLLANDER, M. DE
SEPO, E.
EXPÓSITO, R. G.
MENDES, R.
TSAI, S. M.
CARRÓN, V. J.
dc.subject.por.fl_str_mv Endosphere
Metagenome
Metatranscriptome
Plant-microbe interaction
Rizosfera
Rhizosphere
topic Endosphere
Metagenome
Metatranscriptome
Plant-microbe interaction
Rizosfera
Rhizosphere
description Plants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphere and endosphere microbiomes of a foxsusceptible and fox-resistant common bean cultivar. Integration of 16S-amplicon, shotgun metagenome as well as metatranscriptome sequencing with community ecology analysis showed that fox infections significantly changed the composition and gene expression of the root microbiome in a cultivar-dependent manner. More specifically, fox infection led to increased microbial diversity, network complexity, and a higher proportion of the genera Flavobacterium, Bacillus, and Dyadobacter in the rhizosphere of the fox-resistant cultivar compared to the fox-susceptible cultivar. In the endosphere, root infection also led to changes in community assembly, with a higher abundance of the genera Sinorhizobium and Ensifer in the fox-resistant cultivar. Metagenome and metatranscriptome analyses further revealed the enrichment of terpene biosynthesis genes with a potential role in pathogen suppression in the fox-resistant cultivar upon fungal pathogen invasion. Collectively, these results revealed a cultivar-dependent enrichment of specific bacterial genera and the activation of putative disease-suppressive functions in the rhizosphere and endosphere microbiome of common bean under siege.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024-03-15T13:57:43Z
2024-03-15T13:57:43Z
2024-03-15
dc.type.driver.fl_str_mv Resumo em anais e proceedings
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv In: PLANT MICROBIOME SYMPOSIUM, 4., 2023, Quito. Abstracts... Quito, Equador: Universidad San Francisco de Quito, 2023. Ref. 2-C.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162855
identifier_str_mv In: PLANT MICROBIOME SYMPOSIUM, 4., 2023, Quito. Abstracts... Quito, Equador: Universidad San Francisco de Quito, 2023. Ref. 2-C.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162855
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1 p.
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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