Effect of different gonadotropins on in vitro maturation of sheep oocytes

• Autor(a) principal: Bastos, Bruna Dias Mangueira
• Data de Publicação: 2023
• Outros Autores: Silva, Maria Naiara Pereira da, Gonçalves, Pricila Ramos, Cândido, Ana Elisa Custódio Montes, Barberino, Ricássio de Sousa, Monte, Alane Pains Oliveira do, Nogueira, Daniel Maia, Cordeiro, Mabel Freitas, Matos, Maria Helena Tavares de, Lopes Júnior, Edilson Soares
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Semina. Ciências Agrárias (Online)
• Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/46440
• topic: Follicle-stimulating hormone; Metaphase II.; Hoechst 33342; GSH.; Hormônio folículo estimulante; Metafase II; Hoechst 33342; GSH.

The aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 µg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes.