DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).

Detalhes bibliográficos
Autor(a) principal: MALAUSA, T.
Data de Publicação: 2011
Outros Autores: FENNIS, A., WAROT, S., GERMAIN, J.-F., RIS, N., PRADO, E., BOTTON, M., VANLERBERGHE-MASUTTI, F., SFORZA, R., CRUAUD, C., COULOUX, A., KREITER, P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/875094
Resumo: Mealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the genetic characterization and identification of complexes of taxa in the Pseudococcidae. We surveyed and tested the genetic markers used in previous studies and then identified new markers for particularly relevant genomic regions for which no satisfactory markers were available. We tested all markers on a subset of four taxa distributed worldwide. Five markers were retained after this first screening: two regions of the mitochondrial cytochrome oxidase I gene, 28S-D2, the entire internal transcriber space 2 locus and the rpS15-16S region of the primary mealybug endosymbiont Tremblaya princeps. We then assessed the utility of these markers for the characterization and identification of 239 samples from 43 sites in France and Brazil. The five markers studied (i) successfully distinguished all species identified by morphological examination, (ii) disentangled complexes of species by revealing intraspecific genetic variation and identified a set of closely related taxa for which taxonomic status requires clarification through further studies, and (iii) facilitated the inference of phylogenetic relationships between the characterized taxa.
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spelling DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).Identificação genéticaCaracterização molecularEntomologiaInsetoCochonilhaGenéticaDNAMarcador MolecularMealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the genetic characterization and identification of complexes of taxa in the Pseudococcidae. We surveyed and tested the genetic markers used in previous studies and then identified new markers for particularly relevant genomic regions for which no satisfactory markers were available. We tested all markers on a subset of four taxa distributed worldwide. Five markers were retained after this first screening: two regions of the mitochondrial cytochrome oxidase I gene, 28S-D2, the entire internal transcriber space 2 locus and the rpS15-16S region of the primary mealybug endosymbiont Tremblaya princeps. We then assessed the utility of these markers for the characterization and identification of 239 samples from 43 sites in France and Brazil. The five markers studied (i) successfully distinguished all species identified by morphological examination, (ii) disentangled complexes of species by revealing intraspecific genetic variation and identified a set of closely related taxa for which taxonomic status requires clarification through further studies, and (iii) facilitated the inference of phylogenetic relationships between the characterized taxa.TIBAULT MALAUSA, INRA; A. FENNIS, INRA; S. WAROT, INRA; J.-F. GERMAIN, LNPV MONTPELLIER; N. RIS, INRA; E. PRADO, EPAMIG; MARCOS BOTTON, CNPUV; F. VANLERBERGHE-MASUTTI, INRA; R. SFORZA, EUROPEAN BIOLOGICAL CONTROL LABORATORY; C. CRUAUD, GÉNOSCOPE; A. COULOUX, GÉNOSCOPE; P. KREITER, INRA.MALAUSA, T.FENNIS, A.WAROT, S.GERMAIN, J.-F.RIS, N.PRADO, E.BOTTON, M.VANLERBERGHE-MASUTTI, F.SFORZA, R.CRUAUD, C.COULOUX, A.KREITER, P.2016-10-26T21:35:54Z2016-10-26T21:35:54Z2011-01-3120112019-05-20T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article142-155Journal of Applied Entomology, Hamburg, v. 135, n. 1/2, p. 142-155, fev. 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/87509410.1111/j.1439-0418.2009.01495.xenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:40:23Zoai:www.alice.cnptia.embrapa.br:doc/875094Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:40:23falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:40:23Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).
title DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).
spellingShingle DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).
MALAUSA, T.
Identificação genética
Caracterização molecular
Entomologia
Inseto
Cochonilha
Genética
DNA
Marcador Molecular
title_short DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).
title_full DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).
title_fullStr DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).
title_full_unstemmed DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).
title_sort DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).
author MALAUSA, T.
author_facet MALAUSA, T.
FENNIS, A.
WAROT, S.
GERMAIN, J.-F.
RIS, N.
PRADO, E.
BOTTON, M.
VANLERBERGHE-MASUTTI, F.
SFORZA, R.
CRUAUD, C.
COULOUX, A.
KREITER, P.
author_role author
author2 FENNIS, A.
WAROT, S.
GERMAIN, J.-F.
RIS, N.
PRADO, E.
BOTTON, M.
VANLERBERGHE-MASUTTI, F.
SFORZA, R.
CRUAUD, C.
COULOUX, A.
KREITER, P.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv TIBAULT MALAUSA, INRA; A. FENNIS, INRA; S. WAROT, INRA; J.-F. GERMAIN, LNPV MONTPELLIER; N. RIS, INRA; E. PRADO, EPAMIG; MARCOS BOTTON, CNPUV; F. VANLERBERGHE-MASUTTI, INRA; R. SFORZA, EUROPEAN BIOLOGICAL CONTROL LABORATORY; C. CRUAUD, GÉNOSCOPE; A. COULOUX, GÉNOSCOPE; P. KREITER, INRA.
dc.contributor.author.fl_str_mv MALAUSA, T.
FENNIS, A.
WAROT, S.
GERMAIN, J.-F.
RIS, N.
PRADO, E.
BOTTON, M.
VANLERBERGHE-MASUTTI, F.
SFORZA, R.
CRUAUD, C.
COULOUX, A.
KREITER, P.
dc.subject.por.fl_str_mv Identificação genética
Caracterização molecular
Entomologia
Inseto
Cochonilha
Genética
DNA
Marcador Molecular
topic Identificação genética
Caracterização molecular
Entomologia
Inseto
Cochonilha
Genética
DNA
Marcador Molecular
description Mealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the genetic characterization and identification of complexes of taxa in the Pseudococcidae. We surveyed and tested the genetic markers used in previous studies and then identified new markers for particularly relevant genomic regions for which no satisfactory markers were available. We tested all markers on a subset of four taxa distributed worldwide. Five markers were retained after this first screening: two regions of the mitochondrial cytochrome oxidase I gene, 28S-D2, the entire internal transcriber space 2 locus and the rpS15-16S region of the primary mealybug endosymbiont Tremblaya princeps. We then assessed the utility of these markers for the characterization and identification of 239 samples from 43 sites in France and Brazil. The five markers studied (i) successfully distinguished all species identified by morphological examination, (ii) disentangled complexes of species by revealing intraspecific genetic variation and identified a set of closely related taxa for which taxonomic status requires clarification through further studies, and (iii) facilitated the inference of phylogenetic relationships between the characterized taxa.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-31
2011
2016-10-26T21:35:54Z
2016-10-26T21:35:54Z
2019-05-20T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Journal of Applied Entomology, Hamburg, v. 135, n. 1/2, p. 142-155, fev. 2011.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/875094
10.1111/j.1439-0418.2009.01495.x
identifier_str_mv Journal of Applied Entomology, Hamburg, v. 135, n. 1/2, p. 142-155, fev. 2011.
10.1111/j.1439-0418.2009.01495.x
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/875094
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 142-155
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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