DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/875094 |
Resumo: | Mealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the genetic characterization and identification of complexes of taxa in the Pseudococcidae. We surveyed and tested the genetic markers used in previous studies and then identified new markers for particularly relevant genomic regions for which no satisfactory markers were available. We tested all markers on a subset of four taxa distributed worldwide. Five markers were retained after this first screening: two regions of the mitochondrial cytochrome oxidase I gene, 28S-D2, the entire internal transcriber space 2 locus and the rpS15-16S region of the primary mealybug endosymbiont Tremblaya princeps. We then assessed the utility of these markers for the characterization and identification of 239 samples from 43 sites in France and Brazil. The five markers studied (i) successfully distinguished all species identified by morphological examination, (ii) disentangled complexes of species by revealing intraspecific genetic variation and identified a set of closely related taxa for which taxonomic status requires clarification through further studies, and (iii) facilitated the inference of phylogenetic relationships between the characterized taxa. |
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DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).Identificação genéticaCaracterização molecularEntomologiaInsetoCochonilhaGenéticaDNAMarcador MolecularMealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the genetic characterization and identification of complexes of taxa in the Pseudococcidae. We surveyed and tested the genetic markers used in previous studies and then identified new markers for particularly relevant genomic regions for which no satisfactory markers were available. We tested all markers on a subset of four taxa distributed worldwide. Five markers were retained after this first screening: two regions of the mitochondrial cytochrome oxidase I gene, 28S-D2, the entire internal transcriber space 2 locus and the rpS15-16S region of the primary mealybug endosymbiont Tremblaya princeps. We then assessed the utility of these markers for the characterization and identification of 239 samples from 43 sites in France and Brazil. The five markers studied (i) successfully distinguished all species identified by morphological examination, (ii) disentangled complexes of species by revealing intraspecific genetic variation and identified a set of closely related taxa for which taxonomic status requires clarification through further studies, and (iii) facilitated the inference of phylogenetic relationships between the characterized taxa.TIBAULT MALAUSA, INRA; A. FENNIS, INRA; S. WAROT, INRA; J.-F. GERMAIN, LNPV MONTPELLIER; N. RIS, INRA; E. PRADO, EPAMIG; MARCOS BOTTON, CNPUV; F. VANLERBERGHE-MASUTTI, INRA; R. SFORZA, EUROPEAN BIOLOGICAL CONTROL LABORATORY; C. CRUAUD, GÉNOSCOPE; A. COULOUX, GÉNOSCOPE; P. KREITER, INRA.MALAUSA, T.FENNIS, A.WAROT, S.GERMAIN, J.-F.RIS, N.PRADO, E.BOTTON, M.VANLERBERGHE-MASUTTI, F.SFORZA, R.CRUAUD, C.COULOUX, A.KREITER, P.2016-10-26T21:35:54Z2016-10-26T21:35:54Z2011-01-3120112019-05-20T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article142-155Journal of Applied Entomology, Hamburg, v. 135, n. 1/2, p. 142-155, fev. 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/87509410.1111/j.1439-0418.2009.01495.xenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:40:23Zoai:www.alice.cnptia.embrapa.br:doc/875094Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:40:23falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:40:23Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). |
title |
DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). |
spellingShingle |
DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). MALAUSA, T. Identificação genética Caracterização molecular Entomologia Inseto Cochonilha Genética DNA Marcador Molecular |
title_short |
DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). |
title_full |
DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). |
title_fullStr |
DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). |
title_full_unstemmed |
DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). |
title_sort |
DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). |
author |
MALAUSA, T. |
author_facet |
MALAUSA, T. FENNIS, A. WAROT, S. GERMAIN, J.-F. RIS, N. PRADO, E. BOTTON, M. VANLERBERGHE-MASUTTI, F. SFORZA, R. CRUAUD, C. COULOUX, A. KREITER, P. |
author_role |
author |
author2 |
FENNIS, A. WAROT, S. GERMAIN, J.-F. RIS, N. PRADO, E. BOTTON, M. VANLERBERGHE-MASUTTI, F. SFORZA, R. CRUAUD, C. COULOUX, A. KREITER, P. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
TIBAULT MALAUSA, INRA; A. FENNIS, INRA; S. WAROT, INRA; J.-F. GERMAIN, LNPV MONTPELLIER; N. RIS, INRA; E. PRADO, EPAMIG; MARCOS BOTTON, CNPUV; F. VANLERBERGHE-MASUTTI, INRA; R. SFORZA, EUROPEAN BIOLOGICAL CONTROL LABORATORY; C. CRUAUD, GÉNOSCOPE; A. COULOUX, GÉNOSCOPE; P. KREITER, INRA. |
dc.contributor.author.fl_str_mv |
MALAUSA, T. FENNIS, A. WAROT, S. GERMAIN, J.-F. RIS, N. PRADO, E. BOTTON, M. VANLERBERGHE-MASUTTI, F. SFORZA, R. CRUAUD, C. COULOUX, A. KREITER, P. |
dc.subject.por.fl_str_mv |
Identificação genética Caracterização molecular Entomologia Inseto Cochonilha Genética DNA Marcador Molecular |
topic |
Identificação genética Caracterização molecular Entomologia Inseto Cochonilha Genética DNA Marcador Molecular |
description |
Mealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the genetic characterization and identification of complexes of taxa in the Pseudococcidae. We surveyed and tested the genetic markers used in previous studies and then identified new markers for particularly relevant genomic regions for which no satisfactory markers were available. We tested all markers on a subset of four taxa distributed worldwide. Five markers were retained after this first screening: two regions of the mitochondrial cytochrome oxidase I gene, 28S-D2, the entire internal transcriber space 2 locus and the rpS15-16S region of the primary mealybug endosymbiont Tremblaya princeps. We then assessed the utility of these markers for the characterization and identification of 239 samples from 43 sites in France and Brazil. The five markers studied (i) successfully distinguished all species identified by morphological examination, (ii) disentangled complexes of species by revealing intraspecific genetic variation and identified a set of closely related taxa for which taxonomic status requires clarification through further studies, and (iii) facilitated the inference of phylogenetic relationships between the characterized taxa. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-31 2011 2016-10-26T21:35:54Z 2016-10-26T21:35:54Z 2019-05-20T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Applied Entomology, Hamburg, v. 135, n. 1/2, p. 142-155, fev. 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/875094 10.1111/j.1439-0418.2009.01495.x |
identifier_str_mv |
Journal of Applied Entomology, Hamburg, v. 135, n. 1/2, p. 142-155, fev. 2011. 10.1111/j.1439-0418.2009.01495.x |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/875094 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
142-155 |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503427503423488 |