Ligninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.

Detalhes bibliográficos
Autor(a) principal: FERHAN, M.
Data de Publicação: 2012
Outros Autores: LEÃO, A. L., MELO, I. S. de, YAN, N., SAIN, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/943353
http://dx.doi.org/10.4172/2167-7972.1000106
Resumo: ABSTRACT: The potential of ligninases as a green tool for effective valorization of lignin can be shown through enzymatic cocktails containing different lignin degrading enzymes. The present study deals with the screening of potential fungal strains useful for the liquefaction of bark containing lignin. Three different local isolates (Pleurotus ostreatus POS97/14, Pycnoporus sanguineus and the local isolated fungal strain) were selected out of ten different strains for ligninases production. Maximum production of enzymes was observed in the local isolated fungal strain after ten days in submerged fermentation. The isolated fungal strain produces ligninases mainly for manganese peroxidase (MnP). The enzyme oxidized a variety of the usual MnP substrates, including lignin related phenols. Furthermore, the partial purification for MnP was determined by FPLC and the molecular weight was evaluated by SDS-PAGE.
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spelling Ligninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.LigninasesFungal strainP sanguineusSubmerged fermentation, Fast protein liquid chromatographyLccFungoPeroxidaseLigninaLignin peroxidaseFungiLiquid chromatographyPleurotus ostreatusSubmerged fermentationManganese peroxidaseABSTRACT: The potential of ligninases as a green tool for effective valorization of lignin can be shown through enzymatic cocktails containing different lignin degrading enzymes. The present study deals with the screening of potential fungal strains useful for the liquefaction of bark containing lignin. Three different local isolates (Pleurotus ostreatus POS97/14, Pycnoporus sanguineus and the local isolated fungal strain) were selected out of ten different strains for ligninases production. Maximum production of enzymes was observed in the local isolated fungal strain after ten days in submerged fermentation. The isolated fungal strain produces ligninases mainly for manganese peroxidase (MnP). The enzyme oxidized a variety of the usual MnP substrates, including lignin related phenols. Furthermore, the partial purification for MnP was determined by FPLC and the molecular weight was evaluated by SDS-PAGE.MUHAMMAD FERHAN, University of Toronto; ALCIDES LOPES LEÃO, UNESP; ITAMAR SOARES DE MELO, CNPMA; N. YAN, University of Toronto; M. SAIN, University of Toronto.FERHAN, M.LEÃO, A. L.MELO, I. S. deYAN, N.SAIN, M.2012-12-20T11:11:11Z2012-12-20T11:11:11Z2012-12-2020122015-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFermentation Technology, v. 1, n. 5, 2012.http://www.alice.cnptia.embrapa.br/alice/handle/doc/943353http://dx.doi.org/10.4172/2167-7972.1000106enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T01:56:30Zoai:www.alice.cnptia.embrapa.br:doc/943353Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T01:56:30falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T01:56:30Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Ligninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.
title Ligninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.
spellingShingle Ligninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.
FERHAN, M.
Ligninases
Fungal strain
P sanguineus
Submerged fermentation, Fast protein liquid chromatography
Lcc
Fungo
Peroxidase
Lignina
Lignin peroxidase
Fungi
Liquid chromatography
Pleurotus ostreatus
Submerged fermentation
Manganese peroxidase
title_short Ligninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.
title_full Ligninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.
title_fullStr Ligninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.
title_full_unstemmed Ligninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.
title_sort Ligninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.
author FERHAN, M.
author_facet FERHAN, M.
LEÃO, A. L.
MELO, I. S. de
YAN, N.
SAIN, M.
author_role author
author2 LEÃO, A. L.
MELO, I. S. de
YAN, N.
SAIN, M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv MUHAMMAD FERHAN, University of Toronto; ALCIDES LOPES LEÃO, UNESP; ITAMAR SOARES DE MELO, CNPMA; N. YAN, University of Toronto; M. SAIN, University of Toronto.
dc.contributor.author.fl_str_mv FERHAN, M.
LEÃO, A. L.
MELO, I. S. de
YAN, N.
SAIN, M.
dc.subject.por.fl_str_mv Ligninases
Fungal strain
P sanguineus
Submerged fermentation, Fast protein liquid chromatography
Lcc
Fungo
Peroxidase
Lignina
Lignin peroxidase
Fungi
Liquid chromatography
Pleurotus ostreatus
Submerged fermentation
Manganese peroxidase
topic Ligninases
Fungal strain
P sanguineus
Submerged fermentation, Fast protein liquid chromatography
Lcc
Fungo
Peroxidase
Lignina
Lignin peroxidase
Fungi
Liquid chromatography
Pleurotus ostreatus
Submerged fermentation
Manganese peroxidase
description ABSTRACT: The potential of ligninases as a green tool for effective valorization of lignin can be shown through enzymatic cocktails containing different lignin degrading enzymes. The present study deals with the screening of potential fungal strains useful for the liquefaction of bark containing lignin. Three different local isolates (Pleurotus ostreatus POS97/14, Pycnoporus sanguineus and the local isolated fungal strain) were selected out of ten different strains for ligninases production. Maximum production of enzymes was observed in the local isolated fungal strain after ten days in submerged fermentation. The isolated fungal strain produces ligninases mainly for manganese peroxidase (MnP). The enzyme oxidized a variety of the usual MnP substrates, including lignin related phenols. Furthermore, the partial purification for MnP was determined by FPLC and the molecular weight was evaluated by SDS-PAGE.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-20T11:11:11Z
2012-12-20T11:11:11Z
2012-12-20
2012
2015-04-10T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Fermentation Technology, v. 1, n. 5, 2012.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/943353
http://dx.doi.org/10.4172/2167-7972.1000106
identifier_str_mv Fermentation Technology, v. 1, n. 5, 2012.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/943353
http://dx.doi.org/10.4172/2167-7972.1000106
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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