Comparison of volatile profile and antioxidant activity of Piper divaricatum G. Meyer (Piperaceae) using cuttings and cell tissue.

• Autor(a) principal: CORPES, R.
• Data de Publicação: 2019
• Outros Autores: MONTEIRO, L., ANDRADE, E. H., MAIA, J. G., MENEZES, I. C. de, SETZER, W. N., RAMOS, A. R., SILVA, J. K. R. da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116857; https://doi.org/10.21577/0103-5053.20190128
• topic: Propagação Vegetativa; Micropropagação; Antioxidante; Piperaceae

In this study, P. divaricatum (Piperaceae) plants were subcultured by cuttings and cell tissue in order to carry out a comparison of the volatile profile, phenolic compounds, and antioxidant activity. Propagation by cuttings used vermiculite substrate and Murashige-Skoog (MS) medium in the absence of growth regulators and rooting started at 15 days of growth providing a higher number of plants after 90 days. In vitro propagation was performed using shoot apices as explants in MS media supplemented with 0.5 mg mL-1 BA (6-benzyladenine). Volatile profiles analyzed by gas chromatography-mass spectrometry (GC-MS) showed as main compounds methyl eugenol, E-?-ocimene, and ?-elemene in the in vivo and in vitro cultures. Phenolic contents determined by the Folin-Ciocalteu method had no significant difference at the end of 90 days of growth displaying a good linear correlation with antioxidant activity and phenylpropanoids amounts (r > 0.7). However, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging was higher for in vitro plants grown in comparison to in vivo plants cultured.