Comparison of Volatile Profile and Antioxidant Activity of Piper divaricatum G. Meyer (Piperaceae) Using Cuttings and Cell Tissue

• Autor(a) principal: Corpes,Rosana
• Data de Publicação: 2019
• Outros Autores: Monteiro,Lidiene, Andrade,Eloísa H., Maia,José G., Menezes,Ilmarina, Setzer,William N., Ramos,Alessandra R., Silva,Joyce K. R. da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019001102291
• topic: vegetative propagation; micropropagation; essential oil; phenylpropanoids; phenolic compounds

In this study, P. divaricatum (Piperaceae) plants were subcultured by cuttings and cell tissue in order to carry out a comparison of the volatile profile, phenolic compounds, and antioxidant activity. Propagation by cuttings used vermiculite substrate and Murashige-Skoog (MS) medium in the absence of growth regulators and rooting started at 15 days of growth providing a higher number of plants after 90 days. In vitro propagation was performed using shoot apices as explants in MS media supplemented with 0.5 mg mL-1 BA (6-benzyladenine). Volatile profiles analyzed by gas chromatography-mass spectrometry (GC-MS) showed as main compounds methyl eugenol, E-β-ocimene, and β-elemene in the in vivo and in vitro cultures. Phenolic contents determined by the Folin-Ciocalteu method had no significant difference at the end of 90 days of growth displaying a good linear correlation with antioxidant activity and phenylpropanoids amounts (r > 0.7). However, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging was higher for in vitro plants grown in comparison to in vivo plants cultured.