Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/942711 |
Resumo: | ABSTRACT: The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28°C for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II). The identification of EPS I and EPS II was conducted using FT-IR, 1H, 13C and DEPT-135 NMR spectra. The two substances were identified as linear dextran ? polysaccharides (1 ? 6) which indicated different characteristics with respect to thermal analysis and density of free packaging, viscosity and time of solubilization. Both dextrans are of low density, possess high thermal stability and exhibited the behavior characteristic of pseudoplastic polymers. |
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Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain.ExopolysaccharidePolymerBactériaLeuconostoc pseudomesenteroidesBiopolymersdextranABSTRACT: The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28°C for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II). The identification of EPS I and EPS II was conducted using FT-IR, 1H, 13C and DEPT-135 NMR spectra. The two substances were identified as linear dextran ? polysaccharides (1 ? 6) which indicated different characteristics with respect to thermal analysis and density of free packaging, viscosity and time of solubilization. Both dextrans are of low density, possess high thermal stability and exhibited the behavior characteristic of pseudoplastic polymers.ELINALVA M. PAULO, UEFS; ELISANGELA F. BOFFO, UFBA; ALEXSANDRO BRANCO, UEFS; ÂNGELA M. M. P. VALENTE; ITAMAR SOARES DE MELO, CNPMA; ANTONIO G. FERREIRA, UFSCar; MILTON R. A. ROQUE, UFBA; SANDRA A. DE ASSIS, UEFS.PAULO, E. M.BOFFO, E. F.BRANCO, A.VALENTE, A. M. M. P.MELO, I. S. deFERREIRA, A. G.ROQUE, M. R. de A.ASSIS, S. A. de2012-12-14T11:11:11Z2012-12-14T11:11:11Z2012-12-1420122012-12-14T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnais da Academia Brasileira de Ciências, Rio de Janeiro, v. 84, n. 2, p. 495-507, 2012.http://www.alice.cnptia.embrapa.br/alice/handle/doc/942711enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T01:54:51Zoai:www.alice.cnptia.embrapa.br:doc/942711Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T01:54:51falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T01:54:51Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain. |
title |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain. |
spellingShingle |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain. PAULO, E. M. Exopolysaccharide Polymer Bactéria Leuconostoc pseudomesenteroides Biopolymers dextran |
title_short |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain. |
title_full |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain. |
title_fullStr |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain. |
title_full_unstemmed |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain. |
title_sort |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain. |
author |
PAULO, E. M. |
author_facet |
PAULO, E. M. BOFFO, E. F. BRANCO, A. VALENTE, A. M. M. P. MELO, I. S. de FERREIRA, A. G. ROQUE, M. R. de A. ASSIS, S. A. de |
author_role |
author |
author2 |
BOFFO, E. F. BRANCO, A. VALENTE, A. M. M. P. MELO, I. S. de FERREIRA, A. G. ROQUE, M. R. de A. ASSIS, S. A. de |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
ELINALVA M. PAULO, UEFS; ELISANGELA F. BOFFO, UFBA; ALEXSANDRO BRANCO, UEFS; ÂNGELA M. M. P. VALENTE; ITAMAR SOARES DE MELO, CNPMA; ANTONIO G. FERREIRA, UFSCar; MILTON R. A. ROQUE, UFBA; SANDRA A. DE ASSIS, UEFS. |
dc.contributor.author.fl_str_mv |
PAULO, E. M. BOFFO, E. F. BRANCO, A. VALENTE, A. M. M. P. MELO, I. S. de FERREIRA, A. G. ROQUE, M. R. de A. ASSIS, S. A. de |
dc.subject.por.fl_str_mv |
Exopolysaccharide Polymer Bactéria Leuconostoc pseudomesenteroides Biopolymers dextran |
topic |
Exopolysaccharide Polymer Bactéria Leuconostoc pseudomesenteroides Biopolymers dextran |
description |
ABSTRACT: The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28°C for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II). The identification of EPS I and EPS II was conducted using FT-IR, 1H, 13C and DEPT-135 NMR spectra. The two substances were identified as linear dextran ? polysaccharides (1 ? 6) which indicated different characteristics with respect to thermal analysis and density of free packaging, viscosity and time of solubilization. Both dextrans are of low density, possess high thermal stability and exhibited the behavior characteristic of pseudoplastic polymers. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-14T11:11:11Z 2012-12-14T11:11:11Z 2012-12-14 2012 2012-12-14T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 84, n. 2, p. 495-507, 2012. http://www.alice.cnptia.embrapa.br/alice/handle/doc/942711 |
identifier_str_mv |
Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 84, n. 2, p. 495-507, 2012. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/942711 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503373243809792 |