Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/993458 |
Resumo: | Strain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics and gene-expression assays ? resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean. |
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Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosisTwo-dimensional proteomicsRT-qPCRBradyrhizobiumnitrogen fixationsymbiosisStrain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics and gene-expression assays ? resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean.DOUGLAS FABIANO GOMES, Departamento de Génetica UFPR; JESSIANE STEFÂNIA DA SILVA BATISTA, DEBIOGEM UEPG; AMANDA ALVES PAIVA ROLLA; LUCIANO PAULINO DA SILVA, CENARGEN; CARLOS BLOCH JUNIOR, Cenargen; LYGIA VITORIA GALLI TERASAWA, Departamento de Génetica UFPR; MARIANGELA HUNGRIA DA CUNHA, CNPSO.GOMES, D. F.BATISTA, J. S. da S.ROLLA, A. A. P.SILVA, L. P. daBLOCH, C.GALLI-TARASAWA, L. V.HUNGRIA, M.2017-06-19T23:53:09Z2017-06-19T23:53:09Z2014-08-2620142017-06-19T23:53:09Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBMC Genomics, v. 15, n. 643, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/99345810.1186/1471-2164-15-643porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:32:48Zoai:www.alice.cnptia.embrapa.br:doc/993458Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:32:48falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:32:48Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis |
title |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis |
spellingShingle |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis GOMES, D. F. Two-dimensional proteomics RT-qPCR Bradyrhizobium nitrogen fixation symbiosis |
title_short |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis |
title_full |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis |
title_fullStr |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis |
title_full_unstemmed |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis |
title_sort |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis |
author |
GOMES, D. F. |
author_facet |
GOMES, D. F. BATISTA, J. S. da S. ROLLA, A. A. P. SILVA, L. P. da BLOCH, C. GALLI-TARASAWA, L. V. HUNGRIA, M. |
author_role |
author |
author2 |
BATISTA, J. S. da S. ROLLA, A. A. P. SILVA, L. P. da BLOCH, C. GALLI-TARASAWA, L. V. HUNGRIA, M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
DOUGLAS FABIANO GOMES, Departamento de Génetica UFPR; JESSIANE STEFÂNIA DA SILVA BATISTA, DEBIOGEM UEPG; AMANDA ALVES PAIVA ROLLA; LUCIANO PAULINO DA SILVA, CENARGEN; CARLOS BLOCH JUNIOR, Cenargen; LYGIA VITORIA GALLI TERASAWA, Departamento de Génetica UFPR; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
dc.contributor.author.fl_str_mv |
GOMES, D. F. BATISTA, J. S. da S. ROLLA, A. A. P. SILVA, L. P. da BLOCH, C. GALLI-TARASAWA, L. V. HUNGRIA, M. |
dc.subject.por.fl_str_mv |
Two-dimensional proteomics RT-qPCR Bradyrhizobium nitrogen fixation symbiosis |
topic |
Two-dimensional proteomics RT-qPCR Bradyrhizobium nitrogen fixation symbiosis |
description |
Strain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics and gene-expression assays ? resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-26 2014 2017-06-19T23:53:09Z 2017-06-19T23:53:09Z 2017-06-19T23:53:09Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BMC Genomics, v. 15, n. 643, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/993458 10.1186/1471-2164-15-643 |
identifier_str_mv |
BMC Genomics, v. 15, n. 643, 2014. 10.1186/1471-2164-15-643 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/993458 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503438193655808 |