Speciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001777 |
Resumo: | Selenium is essential for the vital functions of the human and animal organism, such as growth, reproduction, prevention, and the protection of its muscular integrity. Although this dependence is well characterized, aspects related to selenium speciation still need to be studied further. This work describes the development of an alternative method for selenium speciation in bovine blood samples using a hyphenated analytical system (HPLC-UV-HG-ICP-OES). The influence of the main parameters related to the detection of Se species, such as separation efficiency, photo-reduction and hydride generation, were studied. Enzymatic reaction with proteinase-K (1 day at 37ºC) was employed for sample preparation. An on-line UV reduction system was adopted to convert the different species of Se to Se(IV). For the chromatographic system, equipped with an anionic exchange column, a phosphate-buffer of 40 mmol L-1 in the pH of 6.0 was used as the mobile phase (flow rate of 1 mL min-1 ) for separation of the different Se species. To increase the sensitivity, hydride generation was used, followed by inductively coupled plasma optical emission spectrometry (ICP-OES) analysis. The developed method for Se speciation in bovine blood samples was performed completely on-line. The limits of detection (LODs) were 9.5, 7.2, 5.8, 3.1, 4.4 ?g L-1 and the limits of quantification (LOQs) were 31.4, 23.9, 20.1, 10.5, and 14.5 ?g L-1 for selenomethylselenocysteine, seleno-DL-methionine, Se(IV), and Se(VI), respectively. The recoveries obtained were 96% for Se(IV), 98% for Se(VI), 91% for selenocystamine, 89% for seleno-cysteine, and 84% for seleno-methionine. |
id |
EMBR_8899f50079485eef355034499c8852f6 |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1001777 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Speciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES.SeBovine bloodEnzymatic estractionSelenium is essential for the vital functions of the human and animal organism, such as growth, reproduction, prevention, and the protection of its muscular integrity. Although this dependence is well characterized, aspects related to selenium speciation still need to be studied further. This work describes the development of an alternative method for selenium speciation in bovine blood samples using a hyphenated analytical system (HPLC-UV-HG-ICP-OES). The influence of the main parameters related to the detection of Se species, such as separation efficiency, photo-reduction and hydride generation, were studied. Enzymatic reaction with proteinase-K (1 day at 37ºC) was employed for sample preparation. An on-line UV reduction system was adopted to convert the different species of Se to Se(IV). For the chromatographic system, equipped with an anionic exchange column, a phosphate-buffer of 40 mmol L-1 in the pH of 6.0 was used as the mobile phase (flow rate of 1 mL min-1 ) for separation of the different Se species. To increase the sensitivity, hydride generation was used, followed by inductively coupled plasma optical emission spectrometry (ICP-OES) analysis. The developed method for Se speciation in bovine blood samples was performed completely on-line. The limits of detection (LODs) were 9.5, 7.2, 5.8, 3.1, 4.4 ?g L-1 and the limits of quantification (LOQs) were 31.4, 23.9, 20.1, 10.5, and 14.5 ?g L-1 for selenomethylselenocysteine, seleno-DL-methionine, Se(IV), and Se(VI), respectively. The recoveries obtained were 96% for Se(IV), 98% for Se(VI), 91% for selenocystamine, 89% for seleno-cysteine, and 84% for seleno-methionine.GIAN PAULO GIOVANNI FRESCHI, Universidade Federal de Alfenas; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.FRESCHI, G. P. G.NOGUEIRA, A. R. de A.2023-04-11T16:03:43Z2023-04-11T16:03:43Z2014-12-052014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAtomic Spectroscopy, v. 35, n. 5, p. 205-212, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001777porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-04-11T16:03:43Zoai:www.alice.cnptia.embrapa.br:doc/1001777Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-04-11T16:03:43falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-04-11T16:03:43Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Speciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES. |
title |
Speciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES. |
spellingShingle |
Speciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES. FRESCHI, G. P. G. Se Bovine blood Enzymatic estraction |
title_short |
Speciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES. |
title_full |
Speciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES. |
title_fullStr |
Speciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES. |
title_full_unstemmed |
Speciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES. |
title_sort |
Speciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES. |
author |
FRESCHI, G. P. G. |
author_facet |
FRESCHI, G. P. G. NOGUEIRA, A. R. de A. |
author_role |
author |
author2 |
NOGUEIRA, A. R. de A. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
GIAN PAULO GIOVANNI FRESCHI, Universidade Federal de Alfenas; ANA RITA DE ARAUJO NOGUEIRA, CPPSE. |
dc.contributor.author.fl_str_mv |
FRESCHI, G. P. G. NOGUEIRA, A. R. de A. |
dc.subject.por.fl_str_mv |
Se Bovine blood Enzymatic estraction |
topic |
Se Bovine blood Enzymatic estraction |
description |
Selenium is essential for the vital functions of the human and animal organism, such as growth, reproduction, prevention, and the protection of its muscular integrity. Although this dependence is well characterized, aspects related to selenium speciation still need to be studied further. This work describes the development of an alternative method for selenium speciation in bovine blood samples using a hyphenated analytical system (HPLC-UV-HG-ICP-OES). The influence of the main parameters related to the detection of Se species, such as separation efficiency, photo-reduction and hydride generation, were studied. Enzymatic reaction with proteinase-K (1 day at 37ºC) was employed for sample preparation. An on-line UV reduction system was adopted to convert the different species of Se to Se(IV). For the chromatographic system, equipped with an anionic exchange column, a phosphate-buffer of 40 mmol L-1 in the pH of 6.0 was used as the mobile phase (flow rate of 1 mL min-1 ) for separation of the different Se species. To increase the sensitivity, hydride generation was used, followed by inductively coupled plasma optical emission spectrometry (ICP-OES) analysis. The developed method for Se speciation in bovine blood samples was performed completely on-line. The limits of detection (LODs) were 9.5, 7.2, 5.8, 3.1, 4.4 ?g L-1 and the limits of quantification (LOQs) were 31.4, 23.9, 20.1, 10.5, and 14.5 ?g L-1 for selenomethylselenocysteine, seleno-DL-methionine, Se(IV), and Se(VI), respectively. The recoveries obtained were 96% for Se(IV), 98% for Se(VI), 91% for selenocystamine, 89% for seleno-cysteine, and 84% for seleno-methionine. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-05 2014 2023-04-11T16:03:43Z 2023-04-11T16:03:43Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Atomic Spectroscopy, v. 35, n. 5, p. 205-212, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001777 |
identifier_str_mv |
Atomic Spectroscopy, v. 35, n. 5, p. 205-212, 2014. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001777 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503543060692992 |