A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
Autor(a) principal: | |
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Data de Publicação: | 1993 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/105490 |
Resumo: | Effect of different temperatures (15, 19, 25 and 29 °C), media type (PDA and Czapek Dox agar) and light on sporulation of a Caribbean pathovar of Colletotrichum gloe-osporioides f.sp. dioscoreae were studied. Czapek Dox agar and temperature of 25 °C were found to be best conditions for supporting growth and sporulation of this organism. Long-term fungal cultures (2 years axenic culture) did not sporulate effectively under the above conditions unless they were first brought into contact with living tissues of Dioscorea. Methods for inoculation of yams with conidiospores of C. gloeosporioides were developed at the plant, mi-croplant and cell levels. In vitro shoot culture clones showed a similar range of disease reactions to the pathovar as field-grown plants. D. alata showed marked sensitivity to the disease while D. esculenta was the most tolerant species followed by D. composita and D. cayenensis. Dioscorea protoplasts responded differently when cultured in the presence of mycelial culture filtrates and Czapek Dox liquid medium. Glasshouse-raised plants developed anthracnose symptoms only after wounding. |
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A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.Colletotrichum gloesporioides fspdioscoreaeCzapek Dox agamicroplantclonesEffect of different temperatures (15, 19, 25 and 29 °C), media type (PDA and Czapek Dox agar) and light on sporulation of a Caribbean pathovar of Colletotrichum gloe-osporioides f.sp. dioscoreae were studied. Czapek Dox agar and temperature of 25 °C were found to be best conditions for supporting growth and sporulation of this organism. Long-term fungal cultures (2 years axenic culture) did not sporulate effectively under the above conditions unless they were first brought into contact with living tissues of Dioscorea. Methods for inoculation of yams with conidiospores of C. gloeosporioides were developed at the plant, mi-croplant and cell levels. In vitro shoot culture clones showed a similar range of disease reactions to the pathovar as field-grown plants. D. alata showed marked sensitivity to the disease while D. esculenta was the most tolerant species followed by D. composita and D. cayenensis. Dioscorea protoplasts responded differently when cultured in the presence of mycelial culture filtrates and Czapek Dox liquid medium. Glasshouse-raised plants developed anthracnose symptoms only after wounding.2011-04-09T12:13:38Z2011-04-09T12:13:38Z2001-08-2219932011-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Agropecuaria Brasileira, Brasilia, v.28, n.11, p.1297-1306,nov.1993http://www.alice.cnptia.embrapa.br/alice/handle/doc/105490engMOURA-COSA, P.H.MANTELL, S. H.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T21:37:36Zoai:www.alice.cnptia.embrapa.br:doc/105490Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T21:37:36falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T21:37:36Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams. |
title |
A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams. |
spellingShingle |
A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams. MOURA-COSA, P.H. Colletotrichum gloesporioides f sp dioscoreae Czapek Dox aga microplant clones |
title_short |
A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams. |
title_full |
A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams. |
title_fullStr |
A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams. |
title_full_unstemmed |
A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams. |
title_sort |
A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams. |
author |
MOURA-COSA, P.H. |
author_facet |
MOURA-COSA, P.H. MANTELL, S. H. |
author_role |
author |
author2 |
MANTELL, S. H. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
MOURA-COSA, P.H. MANTELL, S. H. |
dc.subject.por.fl_str_mv |
Colletotrichum gloesporioides f sp dioscoreae Czapek Dox aga microplant clones |
topic |
Colletotrichum gloesporioides f sp dioscoreae Czapek Dox aga microplant clones |
description |
Effect of different temperatures (15, 19, 25 and 29 °C), media type (PDA and Czapek Dox agar) and light on sporulation of a Caribbean pathovar of Colletotrichum gloe-osporioides f.sp. dioscoreae were studied. Czapek Dox agar and temperature of 25 °C were found to be best conditions for supporting growth and sporulation of this organism. Long-term fungal cultures (2 years axenic culture) did not sporulate effectively under the above conditions unless they were first brought into contact with living tissues of Dioscorea. Methods for inoculation of yams with conidiospores of C. gloeosporioides were developed at the plant, mi-croplant and cell levels. In vitro shoot culture clones showed a similar range of disease reactions to the pathovar as field-grown plants. D. alata showed marked sensitivity to the disease while D. esculenta was the most tolerant species followed by D. composita and D. cayenensis. Dioscorea protoplasts responded differently when cultured in the presence of mycelial culture filtrates and Czapek Dox liquid medium. Glasshouse-raised plants developed anthracnose symptoms only after wounding. |
publishDate |
1993 |
dc.date.none.fl_str_mv |
1993 2001-08-22 2011-04-09T12:13:38Z 2011-04-09T12:13:38Z 2011-04-10T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Pesquisa Agropecuaria Brasileira, Brasilia, v.28, n.11, p.1297-1306,nov.1993 http://www.alice.cnptia.embrapa.br/alice/handle/doc/105490 |
identifier_str_mv |
Pesquisa Agropecuaria Brasileira, Brasilia, v.28, n.11, p.1297-1306,nov.1993 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/105490 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503315607781376 |