A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.

Detalhes bibliográficos
Autor(a) principal: MOURA-COSA, P.H.
Data de Publicação: 1993
Outros Autores: MANTELL, S. H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/105490
Resumo: Effect of different temperatures (15, 19, 25 and 29 °C), media type (PDA and Czapek Dox agar) and light on sporulation of a Caribbean pathovar of Colletotrichum gloe-osporioides f.sp. dioscoreae were studied. Czapek Dox agar and temperature of 25 °C were found to be best conditions for supporting growth and sporulation of this organism. Long-term fungal cultures (2 years axenic culture) did not sporulate effectively under the above conditions unless they were first brought into contact with living tissues of Dioscorea. Methods for inoculation of yams with conidiospores of C. gloeosporioides were developed at the plant, mi-croplant and cell levels. In vitro shoot culture clones showed a similar range of disease reactions to the pathovar as field-grown plants. D. alata showed marked sensitivity to the disease while D. esculenta was the most tolerant species followed by D. composita and D. cayenensis. Dioscorea protoplasts responded differently when cultured in the presence of mycelial culture filtrates and Czapek Dox liquid medium. Glasshouse-raised plants developed anthracnose symptoms only after wounding.
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spelling A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.Colletotrichum gloesporioides fspdioscoreaeCzapek Dox agamicroplantclonesEffect of different temperatures (15, 19, 25 and 29 °C), media type (PDA and Czapek Dox agar) and light on sporulation of a Caribbean pathovar of Colletotrichum gloe-osporioides f.sp. dioscoreae were studied. Czapek Dox agar and temperature of 25 °C were found to be best conditions for supporting growth and sporulation of this organism. Long-term fungal cultures (2 years axenic culture) did not sporulate effectively under the above conditions unless they were first brought into contact with living tissues of Dioscorea. Methods for inoculation of yams with conidiospores of C. gloeosporioides were developed at the plant, mi-croplant and cell levels. In vitro shoot culture clones showed a similar range of disease reactions to the pathovar as field-grown plants. D. alata showed marked sensitivity to the disease while D. esculenta was the most tolerant species followed by D. composita and D. cayenensis. Dioscorea protoplasts responded differently when cultured in the presence of mycelial culture filtrates and Czapek Dox liquid medium. Glasshouse-raised plants developed anthracnose symptoms only after wounding.2011-04-09T12:13:38Z2011-04-09T12:13:38Z2001-08-2219932011-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Agropecuaria Brasileira, Brasilia, v.28, n.11, p.1297-1306,nov.1993http://www.alice.cnptia.embrapa.br/alice/handle/doc/105490engMOURA-COSA, P.H.MANTELL, S. H.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T21:37:36Zoai:www.alice.cnptia.embrapa.br:doc/105490Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T21:37:36falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T21:37:36Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
title A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
spellingShingle A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
MOURA-COSA, P.H.
Colletotrichum gloesporioides f
sp
dioscoreae
Czapek Dox aga
microplant
clones
title_short A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
title_full A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
title_fullStr A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
title_full_unstemmed A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
title_sort A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
author MOURA-COSA, P.H.
author_facet MOURA-COSA, P.H.
MANTELL, S. H.
author_role author
author2 MANTELL, S. H.
author2_role author
dc.contributor.author.fl_str_mv MOURA-COSA, P.H.
MANTELL, S. H.
dc.subject.por.fl_str_mv Colletotrichum gloesporioides f
sp
dioscoreae
Czapek Dox aga
microplant
clones
topic Colletotrichum gloesporioides f
sp
dioscoreae
Czapek Dox aga
microplant
clones
description Effect of different temperatures (15, 19, 25 and 29 °C), media type (PDA and Czapek Dox agar) and light on sporulation of a Caribbean pathovar of Colletotrichum gloe-osporioides f.sp. dioscoreae were studied. Czapek Dox agar and temperature of 25 °C were found to be best conditions for supporting growth and sporulation of this organism. Long-term fungal cultures (2 years axenic culture) did not sporulate effectively under the above conditions unless they were first brought into contact with living tissues of Dioscorea. Methods for inoculation of yams with conidiospores of C. gloeosporioides were developed at the plant, mi-croplant and cell levels. In vitro shoot culture clones showed a similar range of disease reactions to the pathovar as field-grown plants. D. alata showed marked sensitivity to the disease while D. esculenta was the most tolerant species followed by D. composita and D. cayenensis. Dioscorea protoplasts responded differently when cultured in the presence of mycelial culture filtrates and Czapek Dox liquid medium. Glasshouse-raised plants developed anthracnose symptoms only after wounding.
publishDate 1993
dc.date.none.fl_str_mv 1993
2001-08-22
2011-04-09T12:13:38Z
2011-04-09T12:13:38Z
2011-04-10T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Pesquisa Agropecuaria Brasileira, Brasilia, v.28, n.11, p.1297-1306,nov.1993
http://www.alice.cnptia.embrapa.br/alice/handle/doc/105490
identifier_str_mv Pesquisa Agropecuaria Brasileira, Brasilia, v.28, n.11, p.1297-1306,nov.1993
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/105490
dc.language.iso.fl_str_mv eng
language eng
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dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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