Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063221 |
Resumo: | The aim of this study was to compare the influence of different forces and time of centrifugation in mini-Percoll techniques with traditional Percoll for sperm selection on motility parameters of ram frozen-thawed sperm. For the first time, we have demonstrated that the reduction of the gradient volume and time of centrifugation, and the increase of its force at Percoll, can be successfully used for ram sperm selection. In conclusion, mini-Percoll may be used instead of traditional Percoll, decreasing costs and time of sperm handling, without motility damages in ram frozen-thawed sperm. |
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Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm.Sperm qualityForça e tempo de centrifugaçãoSeleção espermáticaSexagemCinética espermáticaGradiente de PercollMotilidade espermáticaMovemenCarneiroOvinoReprodução animalEspermatozóideSêmenSheepRamsSexual reproductionSpermatozoaThe aim of this study was to compare the influence of different forces and time of centrifugation in mini-Percoll techniques with traditional Percoll for sperm selection on motility parameters of ram frozen-thawed sperm. For the first time, we have demonstrated that the reduction of the gradient volume and time of centrifugation, and the increase of its force at Percoll, can be successfully used for ram sperm selection. In conclusion, mini-Percoll may be used instead of traditional Percoll, decreasing costs and time of sperm handling, without motility damages in ram frozen-thawed sperm.Carolina Olivares; JEFERSON FERREIRA DA FONSECA, CNPC; Helena Saraiva; Vivian Alfradique; Luana Côrtes; Lendel Costa; Felipe Brandão; Joanna Maria Souza-Fabjan.OLIVARES, C.FONSECA, J. F. daSARAIVA, H.ALFRADIQUE, V.CÔRTES, L.COSTA, L.BRANDÃO, F.SOUZA-FABJAN, J. M.2022-05-17T11:14:22Z2022-05-17T11:14:22Z2017-02-082016Resumo em anais e proceedingsinfo:eu-repo/semantics/publishedVersionPoster.In: INTERNATIONAL CONGRESS OF ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tous: Society for Reprocutive Biololy, 2016. p. 168-169. Abstract PW171http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063221enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-05-17T11:14:30Zoai:www.alice.cnptia.embrapa.br:doc/1063221Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-05-17T11:14:30Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm. |
title |
Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm. |
spellingShingle |
Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm. OLIVARES, C. Sperm quality Força e tempo de centrifugação Seleção espermática Sexagem Cinética espermática Gradiente de Percoll Motilidade espermática Movemen Carneiro Ovino Reprodução animal Espermatozóide Sêmen Sheep Rams Sexual reproduction Spermatozoa |
title_short |
Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm. |
title_full |
Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm. |
title_fullStr |
Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm. |
title_full_unstemmed |
Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm. |
title_sort |
Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm. |
author |
OLIVARES, C. |
author_facet |
OLIVARES, C. FONSECA, J. F. da SARAIVA, H. ALFRADIQUE, V. CÔRTES, L. COSTA, L. BRANDÃO, F. SOUZA-FABJAN, J. M. |
author_role |
author |
author2 |
FONSECA, J. F. da SARAIVA, H. ALFRADIQUE, V. CÔRTES, L. COSTA, L. BRANDÃO, F. SOUZA-FABJAN, J. M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Carolina Olivares; JEFERSON FERREIRA DA FONSECA, CNPC; Helena Saraiva; Vivian Alfradique; Luana Côrtes; Lendel Costa; Felipe Brandão; Joanna Maria Souza-Fabjan. |
dc.contributor.author.fl_str_mv |
OLIVARES, C. FONSECA, J. F. da SARAIVA, H. ALFRADIQUE, V. CÔRTES, L. COSTA, L. BRANDÃO, F. SOUZA-FABJAN, J. M. |
dc.subject.por.fl_str_mv |
Sperm quality Força e tempo de centrifugação Seleção espermática Sexagem Cinética espermática Gradiente de Percoll Motilidade espermática Movemen Carneiro Ovino Reprodução animal Espermatozóide Sêmen Sheep Rams Sexual reproduction Spermatozoa |
topic |
Sperm quality Força e tempo de centrifugação Seleção espermática Sexagem Cinética espermática Gradiente de Percoll Motilidade espermática Movemen Carneiro Ovino Reprodução animal Espermatozóide Sêmen Sheep Rams Sexual reproduction Spermatozoa |
description |
The aim of this study was to compare the influence of different forces and time of centrifugation in mini-Percoll techniques with traditional Percoll for sperm selection on motility parameters of ram frozen-thawed sperm. For the first time, we have demonstrated that the reduction of the gradient volume and time of centrifugation, and the increase of its force at Percoll, can be successfully used for ram sperm selection. In conclusion, mini-Percoll may be used instead of traditional Percoll, decreasing costs and time of sperm handling, without motility damages in ram frozen-thawed sperm. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2017-02-08 2022-05-17T11:14:22Z 2022-05-17T11:14:22Z |
dc.type.driver.fl_str_mv |
Resumo em anais e proceedings |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
In: INTERNATIONAL CONGRESS OF ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tous: Society for Reprocutive Biololy, 2016. p. 168-169. Abstract PW171 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063221 |
identifier_str_mv |
In: INTERNATIONAL CONGRESS OF ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tous: Society for Reprocutive Biololy, 2016. p. 168-169. Abstract PW171 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063221 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
Poster. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1817695637232156672 |