CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIO

Detalhes bibliográficos
Autor(a) principal: Resende, Max Vitória
Data de Publicação: 2015
Outros Autores: Biscarde, Carmo Emanuel Almeida, Martins, Laura Emília Panelli, Kiya, Cláudia Kazumi, Hossepian de Lima, Vera Fernanda Martins, Gusmão, Alberto Lopes
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/24607
Resumo: The objective of this study was to evaluate the efficiency of continuous density gradients of Percoll and OptiPrep for separation of X-bearing sperm, and assess the influence on sperm viability, pregnancy rate and sex ratio. With this purpose 100 million spermatozoa were deposited into each tube containing density gradient, which were previously prepared in polystyrene tubes, 24 hours before centrifugation and stored at 4 °C. The tubes were centrifuged at 500xg for 15 minutes at 22 °C, and then the supernatants aspirated and sperm recovered from the bottom of the tubes. A sample was evaluated before and after centrifugation to verify motility and sperm vigor. The sperm were used for fixed time artificial insemination. The results indicated that centrifugation did not affect sperm viability and pregnancy rate, however there was no deviation of sex ratio for females. More studies are needed of adapting the centrifugation technique to sheep and thus obtain the deviation of sex ration as in bovine.Keywords: ram; semen; sexing; sperm viability.
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spelling CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIOCENTRIFUGAÇÃO DE ESPERMATOZOIDES OVINOS EM GRADIENTE DE DENSIDADE CONTÍNUO: EFEITO SOBRE A TAXA DE PRENHEZ E PROPORÇÃO DO SEXOAnimal ReproductionCarneiroSêmenSexagemViabilidade EspermáticaThe objective of this study was to evaluate the efficiency of continuous density gradients of Percoll and OptiPrep for separation of X-bearing sperm, and assess the influence on sperm viability, pregnancy rate and sex ratio. With this purpose 100 million spermatozoa were deposited into each tube containing density gradient, which were previously prepared in polystyrene tubes, 24 hours before centrifugation and stored at 4 °C. The tubes were centrifuged at 500xg for 15 minutes at 22 °C, and then the supernatants aspirated and sperm recovered from the bottom of the tubes. A sample was evaluated before and after centrifugation to verify motility and sperm vigor. The sperm were used for fixed time artificial insemination. The results indicated that centrifugation did not affect sperm viability and pregnancy rate, however there was no deviation of sex ratio for females. More studies are needed of adapting the centrifugation technique to sheep and thus obtain the deviation of sex ration as in bovine.Keywords: ram; semen; sexing; sperm viability.Este trabalho teve como objetivo avaliar a eficiência de gradientes de densidade contínuos de Percoll e OptiPrep para a separação de espermatozoides portadores do cromossomo X, e avaliar a influência sobre a viabilidade espermática, taxa de prenhez e proporção do sexo. Para isso, 100 milhões de espermatozoides foram depositados em cada tubo contendo o gradiente de densidade, previamente preparados, em tubos de poliestireno, 24 horas antes da centrifugação e mantidos a 4 °C. Centrifugou-se a 500 x g por 15 minutos a 22 °C e, posteriormente, os sobrenadantes foram aspirados e os espermatozoides recuperados no fundo dos tubos. Uma amostra foi avaliada antes a após a centrifugação para verificação da motilidade e vigor espermático. Os espermatozoides foram utilizados para a inseminação artificial em tempo fixo. Os resultados indicaram que a centrifugação não afetou a viabilidade espermática e a taxa de prenhez, entretanto não houve desvio da proporção do sexo para fêmeas. Mais estudos são necessários para determinar a necessidade de adaptação da técnica de centrifugação para a espécie ovina e assim obter o desvio da proporção do sexo como na espécie bovina.Palavras-chave: carneiro; sêmen; sexagem; viabilidade espermática. Universidade Federal de Goiás2015-01-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2460710.1590/cab.v16i1.24607Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 1 (2015); 125-132Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 1 (2015); 125-1321809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/24607/17877https://revistas.ufg.br/vet/article/view/24607/17878Resende, Max VitóriaBiscarde, Carmo Emanuel AlmeidaMartins, Laura Emília PanelliKiya, Cláudia KazumiHossepian de Lima, Vera Fernanda MartinsGusmão, Alberto Lopesinfo:eu-repo/semantics/openAccess2015-01-31T19:28:08Zoai:ojs.revistas.ufg.br:article/24607Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:59.946635Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIO
CENTRIFUGAÇÃO DE ESPERMATOZOIDES OVINOS EM GRADIENTE DE DENSIDADE CONTÍNUO: EFEITO SOBRE A TAXA DE PRENHEZ E PROPORÇÃO DO SEXO
title CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIO
spellingShingle CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIO
Resende, Max Vitória
Animal Reproduction
Carneiro
Sêmen
Sexagem
Viabilidade Espermática
title_short CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIO
title_full CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIO
title_fullStr CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIO
title_full_unstemmed CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIO
title_sort CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIO
author Resende, Max Vitória
author_facet Resende, Max Vitória
Biscarde, Carmo Emanuel Almeida
Martins, Laura Emília Panelli
Kiya, Cláudia Kazumi
Hossepian de Lima, Vera Fernanda Martins
Gusmão, Alberto Lopes
author_role author
author2 Biscarde, Carmo Emanuel Almeida
Martins, Laura Emília Panelli
Kiya, Cláudia Kazumi
Hossepian de Lima, Vera Fernanda Martins
Gusmão, Alberto Lopes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Resende, Max Vitória
Biscarde, Carmo Emanuel Almeida
Martins, Laura Emília Panelli
Kiya, Cláudia Kazumi
Hossepian de Lima, Vera Fernanda Martins
Gusmão, Alberto Lopes
dc.subject.por.fl_str_mv Animal Reproduction
Carneiro
Sêmen
Sexagem
Viabilidade Espermática
topic Animal Reproduction
Carneiro
Sêmen
Sexagem
Viabilidade Espermática
description The objective of this study was to evaluate the efficiency of continuous density gradients of Percoll and OptiPrep for separation of X-bearing sperm, and assess the influence on sperm viability, pregnancy rate and sex ratio. With this purpose 100 million spermatozoa were deposited into each tube containing density gradient, which were previously prepared in polystyrene tubes, 24 hours before centrifugation and stored at 4 °C. The tubes were centrifuged at 500xg for 15 minutes at 22 °C, and then the supernatants aspirated and sperm recovered from the bottom of the tubes. A sample was evaluated before and after centrifugation to verify motility and sperm vigor. The sperm were used for fixed time artificial insemination. The results indicated that centrifugation did not affect sperm viability and pregnancy rate, however there was no deviation of sex ratio for females. More studies are needed of adapting the centrifugation technique to sheep and thus obtain the deviation of sex ration as in bovine.Keywords: ram; semen; sexing; sperm viability.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/24607
10.1590/cab.v16i1.24607
url https://revistas.ufg.br/vet/article/view/24607
identifier_str_mv 10.1590/cab.v16i1.24607
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/24607/17877
https://revistas.ufg.br/vet/article/view/24607/17878
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 1 (2015); 125-132
Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 1 (2015); 125-132
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1799874788171186176

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