Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.

Detalhes bibliográficos
Autor(a) principal: ROCHA, E. A.
Data de Publicação: 2010
Outros Autores: PAIVA, L. V., CARVALHO, H. H. de, GUIMARAES, C. T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/871381
Resumo: This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified.
id EMBR_903a2e0904c531c42556353bc5623e34
oai_identifier_str oai:www.alice.cnptia.embrapa.br:doc/871381
network_acronym_str EMBR
network_name_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository_id_str 2154
spelling Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.Diversidade genéticaMarcador molecularBatataThis study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified.ELIZANGELA ALMEIDA ROCHA, UFMG; LUCIANO VILELA PAIVA, UFLA; HUMBERTO HENRIQUE DE CARVALHO, UFV; CLAUDIA TEIXEIRA GUIMARAES, CNPMS.ROCHA, E. A.PAIVA, L. V.CARVALHO, H. H. deGUIMARAES, C. T.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-01-0320102018-05-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, Londrina, v. 10, p. 204-210, 2010.http://www.alice.cnptia.embrapa.br/alice/handle/doc/871381enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:43:46Zoai:www.alice.cnptia.embrapa.br:doc/871381Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:43:46Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.
title Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.
spellingShingle Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.
ROCHA, E. A.
Diversidade genética
Marcador molecular
Batata
title_short Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.
title_full Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.
title_fullStr Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.
title_full_unstemmed Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.
title_sort Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.
author ROCHA, E. A.
author_facet ROCHA, E. A.
PAIVA, L. V.
CARVALHO, H. H. de
GUIMARAES, C. T.
author_role author
author2 PAIVA, L. V.
CARVALHO, H. H. de
GUIMARAES, C. T.
author2_role author
author
author
dc.contributor.none.fl_str_mv ELIZANGELA ALMEIDA ROCHA, UFMG; LUCIANO VILELA PAIVA, UFLA; HUMBERTO HENRIQUE DE CARVALHO, UFV; CLAUDIA TEIXEIRA GUIMARAES, CNPMS.
dc.contributor.author.fl_str_mv ROCHA, E. A.
PAIVA, L. V.
CARVALHO, H. H. de
GUIMARAES, C. T.
dc.subject.por.fl_str_mv Diversidade genética
Marcador molecular
Batata
topic Diversidade genética
Marcador molecular
Batata
description This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified.
publishDate 2010
dc.date.none.fl_str_mv 2010
2011-04-10T11:11:11Z
2011-04-10T11:11:11Z
2011-04-10T11:11:11Z
2011-01-03
2018-05-29T11:11:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Crop Breeding and Applied Biotechnology, Londrina, v. 10, p. 204-210, 2010.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/871381
identifier_str_mv Crop Breeding and Applied Biotechnology, Londrina, v. 10, p. 204-210, 2010.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/871381
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
_version_ 1817695237011668992

Registros relacionados