Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/871381 |
Resumo: | This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified. |
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Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers.Diversidade genéticaMarcador molecularBatataThis study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified.ELIZANGELA ALMEIDA ROCHA, UFMG; LUCIANO VILELA PAIVA, UFLA; HUMBERTO HENRIQUE DE CARVALHO, UFV; CLAUDIA TEIXEIRA GUIMARAES, CNPMS.ROCHA, E. A.PAIVA, L. V.CARVALHO, H. H. deGUIMARAES, C. T.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-01-0320102018-05-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, Londrina, v. 10, p. 204-210, 2010.http://www.alice.cnptia.embrapa.br/alice/handle/doc/871381enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:43:46Zoai:www.alice.cnptia.embrapa.br:doc/871381Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:43:46falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:43:46Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. |
title |
Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. |
spellingShingle |
Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. ROCHA, E. A. Diversidade genética Marcador molecular Batata |
title_short |
Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. |
title_full |
Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. |
title_fullStr |
Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. |
title_full_unstemmed |
Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. |
title_sort |
Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. |
author |
ROCHA, E. A. |
author_facet |
ROCHA, E. A. PAIVA, L. V. CARVALHO, H. H. de GUIMARAES, C. T. |
author_role |
author |
author2 |
PAIVA, L. V. CARVALHO, H. H. de GUIMARAES, C. T. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
ELIZANGELA ALMEIDA ROCHA, UFMG; LUCIANO VILELA PAIVA, UFLA; HUMBERTO HENRIQUE DE CARVALHO, UFV; CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
dc.contributor.author.fl_str_mv |
ROCHA, E. A. PAIVA, L. V. CARVALHO, H. H. de GUIMARAES, C. T. |
dc.subject.por.fl_str_mv |
Diversidade genética Marcador molecular Batata |
topic |
Diversidade genética Marcador molecular Batata |
description |
This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2011-01-03 2018-05-29T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Crop Breeding and Applied Biotechnology, Londrina, v. 10, p. 204-210, 2010. http://www.alice.cnptia.embrapa.br/alice/handle/doc/871381 |
identifier_str_mv |
Crop Breeding and Applied Biotechnology, Londrina, v. 10, p. 204-210, 2010. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/871381 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503361987346432 |