Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers

Detalhes bibliográficos
Autor(a) principal: Rocha, Elizângela Almeida
Data de Publicação: 2010
Outros Autores: Paiva, Luciano Vilela, Carvalho, Humberto Henrique de, Guimarães, Claudia Teixeira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S1984-70332010000300004
http://locus.ufv.br//handle/123456789/25889
Resumo: This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified.
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spelling Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markersSolanum tuberosumRAPDMicrosatellitesGenetic diversityMicrossatélitesDiversidade genéticaThis study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified.Este trabalho teve como objetivo avaliar a divergência genética e identificar cultivares de batata por meio de marcadores RAPD e SSR. O DNA genômico de 16 cultivares de batata foi amplificado com primers RAPD e com pares de primers SSR. Os 25 primers RAPD geraram 92 bandas polimórficas e os 20 pares de primers SSR produziram 136 bandas polimórficas. Os dendrogramas gerados pela análise de agrupamento permitiram uma distinção genética das cultivares, entretanto não houve uma correlaçao entre os dendrogramas ao comparar os dois marcadores utilizados. Os valores de PIC demonstraram o alto conteúdo informativo dos primers utilizados e, com a utilização de 6 primers RAPD e de 3 pares de primers SSR, identificou-se as 16 cultivares de batatas. Dessa forma, por meio de marcadores moleculares RAPD e SSR foi possível avaliar a divergência genética e identificar as 16 cultivares comerciais de batata analisadas neste estudo.Crop Breeding and Applied Biotechnology2019-06-19T14:04:59Z2019-06-19T14:04:59Z2010-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1984-7033http://dx.doi.org/10.1590/S1984-70332010000300004http://locus.ufv.br//handle/123456789/25889engv. 10, n. 3, p. 204- 210, set. 2010Rocha, Elizângela AlmeidaPaiva, Luciano VilelaCarvalho, Humberto Henrique deGuimarães, Claudia Teixeirainfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:37:10Zoai:locus.ufv.br:123456789/25889Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:37:10LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers
title Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers
spellingShingle Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers
Rocha, Elizângela Almeida
Solanum tuberosum
RAPD
Microsatellites
Genetic diversity
Microssatélites
Diversidade genética
title_short Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers
title_full Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers
title_fullStr Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers
title_full_unstemmed Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers
title_sort Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers
author Rocha, Elizângela Almeida
author_facet Rocha, Elizângela Almeida
Paiva, Luciano Vilela
Carvalho, Humberto Henrique de
Guimarães, Claudia Teixeira
author_role author
author2 Paiva, Luciano Vilela
Carvalho, Humberto Henrique de
Guimarães, Claudia Teixeira
author2_role author
author
author
dc.contributor.author.fl_str_mv Rocha, Elizângela Almeida
Paiva, Luciano Vilela
Carvalho, Humberto Henrique de
Guimarães, Claudia Teixeira
dc.subject.por.fl_str_mv Solanum tuberosum
RAPD
Microsatellites
Genetic diversity
Microssatélites
Diversidade genética
topic Solanum tuberosum
RAPD
Microsatellites
Genetic diversity
Microssatélites
Diversidade genética
description This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
2019-06-19T14:04:59Z
2019-06-19T14:04:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 1984-7033
http://dx.doi.org/10.1590/S1984-70332010000300004
http://locus.ufv.br//handle/123456789/25889
identifier_str_mv 1984-7033
url http://dx.doi.org/10.1590/S1984-70332010000300004
http://locus.ufv.br//handle/123456789/25889
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 10, n. 3, p. 204- 210, set. 2010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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