Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1050554 |
Resumo: | The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility. |
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Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.Encefalopatia espongiforme transmissivelGene PRNPGenotipagemPolimorfismo de nucleotídeo únicoSingle nucleotide polymorphismsTransmissible spongiform encephalopathiesgenotypingprion diseasesThe objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.Notas científicas.ALINE NAJARA DOMINGOS GONÇALVES, Universidade Federal de Mato Grosso do sul; CLEBER OLIVEIRA SOARES, CNPGC; SIMONE CAMARGO SANCHES, Universidade Federal de Mato Grosso do Sul; FERNANDO ALVARENGA REIS, CNPC; GRACIA MARIA SOARES ROSINHA, CNPGC.GONÇALVES, A. N. D.SOARES, C. O.SANCHES, S. C.REIS, F. A.ROSINHA, G. M. S.2016-08-09T11:11:11Z2016-08-09T11:11:11Z2016-08-0920162017-03-07T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 5, p.684-687, maio, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1050554porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:18:54Zoai:www.alice.cnptia.embrapa.br:doc/1050554Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:18:54falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:18:54Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. |
title |
Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. |
spellingShingle |
Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. GONÇALVES, A. N. D. Encefalopatia espongiforme transmissivel Gene PRNP Genotipagem Polimorfismo de nucleotídeo único Single nucleotide polymorphisms Transmissible spongiform encephalopathies genotyping prion diseases |
title_short |
Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. |
title_full |
Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. |
title_fullStr |
Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. |
title_full_unstemmed |
Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. |
title_sort |
Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. |
author |
GONÇALVES, A. N. D. |
author_facet |
GONÇALVES, A. N. D. SOARES, C. O. SANCHES, S. C. REIS, F. A. ROSINHA, G. M. S. |
author_role |
author |
author2 |
SOARES, C. O. SANCHES, S. C. REIS, F. A. ROSINHA, G. M. S. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
ALINE NAJARA DOMINGOS GONÇALVES, Universidade Federal de Mato Grosso do sul; CLEBER OLIVEIRA SOARES, CNPGC; SIMONE CAMARGO SANCHES, Universidade Federal de Mato Grosso do Sul; FERNANDO ALVARENGA REIS, CNPC; GRACIA MARIA SOARES ROSINHA, CNPGC. |
dc.contributor.author.fl_str_mv |
GONÇALVES, A. N. D. SOARES, C. O. SANCHES, S. C. REIS, F. A. ROSINHA, G. M. S. |
dc.subject.por.fl_str_mv |
Encefalopatia espongiforme transmissivel Gene PRNP Genotipagem Polimorfismo de nucleotídeo único Single nucleotide polymorphisms Transmissible spongiform encephalopathies genotyping prion diseases |
topic |
Encefalopatia espongiforme transmissivel Gene PRNP Genotipagem Polimorfismo de nucleotídeo único Single nucleotide polymorphisms Transmissible spongiform encephalopathies genotyping prion diseases |
description |
The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-09T11:11:11Z 2016-08-09T11:11:11Z 2016-08-09 2016 2017-03-07T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Pesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 5, p.684-687, maio, 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1050554 |
identifier_str_mv |
Pesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 5, p.684-687, maio, 2016. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1050554 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503424374472704 |