Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.

Detalhes bibliográficos
Autor(a) principal: GONÇALVES, A. N. D.
Data de Publicação: 2016
Outros Autores: SOARES, C. O., SANCHES, S. C., REIS, F. A., ROSINHA, G. M. S.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1050554
Resumo: The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.
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spelling Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.Encefalopatia espongiforme transmissivelGene PRNPGenotipagemPolimorfismo de nucleotídeo únicoSingle nucleotide polymorphismsTransmissible spongiform encephalopathiesgenotypingprion diseasesThe objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.Notas científicas.ALINE NAJARA DOMINGOS GONÇALVES, Universidade Federal de Mato Grosso do sul; CLEBER OLIVEIRA SOARES, CNPGC; SIMONE CAMARGO SANCHES, Universidade Federal de Mato Grosso do Sul; FERNANDO ALVARENGA REIS, CNPC; GRACIA MARIA SOARES ROSINHA, CNPGC.GONÇALVES, A. N. D.SOARES, C. O.SANCHES, S. C.REIS, F. A.ROSINHA, G. M. S.2016-08-09T11:11:11Z2016-08-09T11:11:11Z2016-08-0920162017-03-07T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 5, p.684-687, maio, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1050554porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:18:54Zoai:www.alice.cnptia.embrapa.br:doc/1050554Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:18:54falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:18:54Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.
title Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.
spellingShingle Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.
GONÇALVES, A. N. D.
Encefalopatia espongiforme transmissivel
Gene PRNP
Genotipagem
Polimorfismo de nucleotídeo único
Single nucleotide polymorphisms
Transmissible spongiform encephalopathies
genotyping
prion diseases
title_short Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.
title_full Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.
title_fullStr Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.
title_full_unstemmed Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.
title_sort Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.
author GONÇALVES, A. N. D.
author_facet GONÇALVES, A. N. D.
SOARES, C. O.
SANCHES, S. C.
REIS, F. A.
ROSINHA, G. M. S.
author_role author
author2 SOARES, C. O.
SANCHES, S. C.
REIS, F. A.
ROSINHA, G. M. S.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv ALINE NAJARA DOMINGOS GONÇALVES, Universidade Federal de Mato Grosso do sul; CLEBER OLIVEIRA SOARES, CNPGC; SIMONE CAMARGO SANCHES, Universidade Federal de Mato Grosso do Sul; FERNANDO ALVARENGA REIS, CNPC; GRACIA MARIA SOARES ROSINHA, CNPGC.
dc.contributor.author.fl_str_mv GONÇALVES, A. N. D.
SOARES, C. O.
SANCHES, S. C.
REIS, F. A.
ROSINHA, G. M. S.
dc.subject.por.fl_str_mv Encefalopatia espongiforme transmissivel
Gene PRNP
Genotipagem
Polimorfismo de nucleotídeo único
Single nucleotide polymorphisms
Transmissible spongiform encephalopathies
genotyping
prion diseases
topic Encefalopatia espongiforme transmissivel
Gene PRNP
Genotipagem
Polimorfismo de nucleotídeo único
Single nucleotide polymorphisms
Transmissible spongiform encephalopathies
genotyping
prion diseases
description The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-09T11:11:11Z
2016-08-09T11:11:11Z
2016-08-09
2016
2017-03-07T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Pesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 5, p.684-687, maio, 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1050554
identifier_str_mv Pesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 5, p.684-687, maio, 2016.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1050554
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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