Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/926619 |
Resumo: | Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress. |
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Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.SojaGenomaGeneDeficiência hídricaRelação água-plantaSecaEstiagemSoybeansGene expressionDroughtSoybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.FABIANA APARECIDA RODRIGUES, CNPSo; JULIANA MARCOLINO-GOMES, CNPSo; JOSIRLEI DE FÁTIMA CORRÊA CARVALHO, CARVALHO , J. de F. C.; LEANDRO COSTA DO NASCIMENTO, UNICAMP; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARCELO FALSARELLA CARAZZOLLE, UNICAMP; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI.RODRIGUES, F. A.MARCOLINO-GOMES, J.CARVALHO, J. de F. C.NASCIMENTO, L. C. doNEUMAIER, N.FARIAS, J. R. B.CARAZZOLLE, M. F.MARCELINO, F. C.NEPOMUCENO, A. L.2012-06-18T11:11:11Z2012-06-18T11:11:11Z2012-06-1820122012-07-24T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 304-314, May 2012.http://www.alice.cnptia.embrapa.br/alice/handle/doc/926619enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:35:19Zoai:www.alice.cnptia.embrapa.br:doc/926619Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:35:19falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:35:19Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit. |
title |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit. |
spellingShingle |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit. RODRIGUES, F. A. Soja Genoma Gene Deficiência hídrica Relação água-planta Seca Estiagem Soybeans Gene expression Drought |
title_short |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit. |
title_full |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit. |
title_fullStr |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit. |
title_full_unstemmed |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit. |
title_sort |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit. |
author |
RODRIGUES, F. A. |
author_facet |
RODRIGUES, F. A. MARCOLINO-GOMES, J. CARVALHO, J. de F. C. NASCIMENTO, L. C. do NEUMAIER, N. FARIAS, J. R. B. CARAZZOLLE, M. F. MARCELINO, F. C. NEPOMUCENO, A. L. |
author_role |
author |
author2 |
MARCOLINO-GOMES, J. CARVALHO, J. de F. C. NASCIMENTO, L. C. do NEUMAIER, N. FARIAS, J. R. B. CARAZZOLLE, M. F. MARCELINO, F. C. NEPOMUCENO, A. L. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
FABIANA APARECIDA RODRIGUES, CNPSo; JULIANA MARCOLINO-GOMES, CNPSo; JOSIRLEI DE FÁTIMA CORRÊA CARVALHO, CARVALHO , J. de F. C.; LEANDRO COSTA DO NASCIMENTO, UNICAMP; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARCELO FALSARELLA CARAZZOLLE, UNICAMP; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI. |
dc.contributor.author.fl_str_mv |
RODRIGUES, F. A. MARCOLINO-GOMES, J. CARVALHO, J. de F. C. NASCIMENTO, L. C. do NEUMAIER, N. FARIAS, J. R. B. CARAZZOLLE, M. F. MARCELINO, F. C. NEPOMUCENO, A. L. |
dc.subject.por.fl_str_mv |
Soja Genoma Gene Deficiência hídrica Relação água-planta Seca Estiagem Soybeans Gene expression Drought |
topic |
Soja Genoma Gene Deficiência hídrica Relação água-planta Seca Estiagem Soybeans Gene expression Drought |
description |
Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-18T11:11:11Z 2012-06-18T11:11:11Z 2012-06-18 2012 2012-07-24T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 304-314, May 2012. http://www.alice.cnptia.embrapa.br/alice/handle/doc/926619 |
identifier_str_mv |
Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 304-314, May 2012. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/926619 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503364870930432 |