Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.

Detalhes bibliográficos
Autor(a) principal: RODRIGUES, F. A.
Data de Publicação: 2012
Outros Autores: MARCOLINO-GOMES, J., CARVALHO, J. de F. C., NASCIMENTO, L. C. do, NEUMAIER, N., FARIAS, J. R. B., CARAZZOLLE, M. F., MARCELINO, F. C., NEPOMUCENO, A. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/926619
Resumo: Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.
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spelling Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.SojaGenomaGeneDeficiência hídricaRelação água-plantaSecaEstiagemSoybeansGene expressionDroughtSoybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.FABIANA APARECIDA RODRIGUES, CNPSo; JULIANA MARCOLINO-GOMES, CNPSo; JOSIRLEI DE FÁTIMA CORRÊA CARVALHO, CARVALHO , J. de F. C.; LEANDRO COSTA DO NASCIMENTO, UNICAMP; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARCELO FALSARELLA CARAZZOLLE, UNICAMP; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI.RODRIGUES, F. A.MARCOLINO-GOMES, J.CARVALHO, J. de F. C.NASCIMENTO, L. C. doNEUMAIER, N.FARIAS, J. R. B.CARAZZOLLE, M. F.MARCELINO, F. C.NEPOMUCENO, A. L.2012-06-18T11:11:11Z2012-06-18T11:11:11Z2012-06-1820122012-07-24T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 304-314, May 2012.http://www.alice.cnptia.embrapa.br/alice/handle/doc/926619enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:35:19Zoai:www.alice.cnptia.embrapa.br:doc/926619Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:35:19falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:35:19Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.
title Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.
spellingShingle Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.
RODRIGUES, F. A.
Soja
Genoma
Gene
Deficiência hídrica
Relação água-planta
Seca
Estiagem
Soybeans
Gene expression
Drought
title_short Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.
title_full Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.
title_fullStr Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.
title_full_unstemmed Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.
title_sort Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.
author RODRIGUES, F. A.
author_facet RODRIGUES, F. A.
MARCOLINO-GOMES, J.
CARVALHO, J. de F. C.
NASCIMENTO, L. C. do
NEUMAIER, N.
FARIAS, J. R. B.
CARAZZOLLE, M. F.
MARCELINO, F. C.
NEPOMUCENO, A. L.
author_role author
author2 MARCOLINO-GOMES, J.
CARVALHO, J. de F. C.
NASCIMENTO, L. C. do
NEUMAIER, N.
FARIAS, J. R. B.
CARAZZOLLE, M. F.
MARCELINO, F. C.
NEPOMUCENO, A. L.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv FABIANA APARECIDA RODRIGUES, CNPSo; JULIANA MARCOLINO-GOMES, CNPSo; JOSIRLEI DE FÁTIMA CORRÊA CARVALHO, CARVALHO , J. de F. C.; LEANDRO COSTA DO NASCIMENTO, UNICAMP; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARCELO FALSARELLA CARAZZOLLE, UNICAMP; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI.
dc.contributor.author.fl_str_mv RODRIGUES, F. A.
MARCOLINO-GOMES, J.
CARVALHO, J. de F. C.
NASCIMENTO, L. C. do
NEUMAIER, N.
FARIAS, J. R. B.
CARAZZOLLE, M. F.
MARCELINO, F. C.
NEPOMUCENO, A. L.
dc.subject.por.fl_str_mv Soja
Genoma
Gene
Deficiência hídrica
Relação água-planta
Seca
Estiagem
Soybeans
Gene expression
Drought
topic Soja
Genoma
Gene
Deficiência hídrica
Relação água-planta
Seca
Estiagem
Soybeans
Gene expression
Drought
description Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-18T11:11:11Z
2012-06-18T11:11:11Z
2012-06-18
2012
2012-07-24T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 304-314, May 2012.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/926619
identifier_str_mv Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 304-314, May 2012.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/926619
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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