Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011 |
Resumo: | Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress. |
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Genetics and Molecular Biology |
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Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficitgene expressiondroughttranscriptomeSoybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.Sociedade Brasileira de Genética2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011Genetics and Molecular Biology v.35 n.1 suppl.1 2012reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572012000200011info:eu-repo/semantics/openAccessRodrigues,Fabiana AparecidaMarcolino-Gomes,JulianaCarvalho,Josirlei de Fátima CorrêaNascimento,Leandro Costa doNeumaier,NormanFarias,José Renato BouçasCarazzolle,Marcelo FalsarellaMarcelino,Francismar CorrêaNepomuceno,Alexandre Limaeng2012-06-01T00:00:00Zoai:scielo:S1415-47572012000200011Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2012-06-01T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit |
title |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit |
spellingShingle |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit Rodrigues,Fabiana Aparecida gene expression drought transcriptome |
title_short |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit |
title_full |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit |
title_fullStr |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit |
title_full_unstemmed |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit |
title_sort |
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit |
author |
Rodrigues,Fabiana Aparecida |
author_facet |
Rodrigues,Fabiana Aparecida Marcolino-Gomes,Juliana Carvalho,Josirlei de Fátima Corrêa Nascimento,Leandro Costa do Neumaier,Norman Farias,José Renato Bouças Carazzolle,Marcelo Falsarella Marcelino,Francismar Corrêa Nepomuceno,Alexandre Lima |
author_role |
author |
author2 |
Marcolino-Gomes,Juliana Carvalho,Josirlei de Fátima Corrêa Nascimento,Leandro Costa do Neumaier,Norman Farias,José Renato Bouças Carazzolle,Marcelo Falsarella Marcelino,Francismar Corrêa Nepomuceno,Alexandre Lima |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues,Fabiana Aparecida Marcolino-Gomes,Juliana Carvalho,Josirlei de Fátima Corrêa Nascimento,Leandro Costa do Neumaier,Norman Farias,José Renato Bouças Carazzolle,Marcelo Falsarella Marcelino,Francismar Corrêa Nepomuceno,Alexandre Lima |
dc.subject.por.fl_str_mv |
gene expression drought transcriptome |
topic |
gene expression drought transcriptome |
description |
Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572012000200011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.35 n.1 suppl.1 2012 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122384561733632 |