Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit

Detalhes bibliográficos
Autor(a) principal: Rodrigues,Fabiana Aparecida
Data de Publicação: 2012
Outros Autores: Marcolino-Gomes,Juliana, Carvalho,Josirlei de Fátima Corrêa, Nascimento,Leandro Costa do, Neumaier,Norman, Farias,José Renato Bouças, Carazzolle,Marcelo Falsarella, Marcelino,Francismar Corrêa, Nepomuceno,Alexandre Lima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011
Resumo: Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.
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spelling Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficitgene expressiondroughttranscriptomeSoybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.Sociedade Brasileira de Genética2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011Genetics and Molecular Biology v.35 n.1 suppl.1 2012reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572012000200011info:eu-repo/semantics/openAccessRodrigues,Fabiana AparecidaMarcolino-Gomes,JulianaCarvalho,Josirlei de Fátima CorrêaNascimento,Leandro Costa doNeumaier,NormanFarias,José Renato BouçasCarazzolle,Marcelo FalsarellaMarcelino,Francismar CorrêaNepomuceno,Alexandre Limaeng2012-06-01T00:00:00Zoai:scielo:S1415-47572012000200011Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2012-06-01T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
title Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
spellingShingle Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
Rodrigues,Fabiana Aparecida
gene expression
drought
transcriptome
title_short Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
title_full Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
title_fullStr Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
title_full_unstemmed Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
title_sort Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
author Rodrigues,Fabiana Aparecida
author_facet Rodrigues,Fabiana Aparecida
Marcolino-Gomes,Juliana
Carvalho,Josirlei de Fátima Corrêa
Nascimento,Leandro Costa do
Neumaier,Norman
Farias,José Renato Bouças
Carazzolle,Marcelo Falsarella
Marcelino,Francismar Corrêa
Nepomuceno,Alexandre Lima
author_role author
author2 Marcolino-Gomes,Juliana
Carvalho,Josirlei de Fátima Corrêa
Nascimento,Leandro Costa do
Neumaier,Norman
Farias,José Renato Bouças
Carazzolle,Marcelo Falsarella
Marcelino,Francismar Corrêa
Nepomuceno,Alexandre Lima
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues,Fabiana Aparecida
Marcolino-Gomes,Juliana
Carvalho,Josirlei de Fátima Corrêa
Nascimento,Leandro Costa do
Neumaier,Norman
Farias,José Renato Bouças
Carazzolle,Marcelo Falsarella
Marcelino,Francismar Corrêa
Nepomuceno,Alexandre Lima
dc.subject.por.fl_str_mv gene expression
drought
transcriptome
topic gene expression
drought
transcriptome
description Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572012000200011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.35 n.1 suppl.1 2012
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
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