Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil.

Detalhes bibliográficos
Autor(a) principal: BIER, D.
Data de Publicação: 2018
Outros Autores: KICH, J. D., DUARTE, S. C., SILVA, M. R., VALSONI, L. M., RAMOS, C. A. N., RODRIGUES, D. P., ARAUJO, F. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102218
Resumo: The aim of the present study was to investigate the presence of Salmonella spp. in samples collected from beef meat at three points of the slaughter line (after skinning, washing and cooling) at three slaughterhouses in Brazil that export meat. Detection was based on ISO 6579:2002 and confirmed by PCR and qPCR. The isolates were typified using slide agglutination tests and PFGE. The antibiotic sensitivity profile was determined using the disk diffusion method. Contamination was detected in only one slaughterhouse. The overall frequency of contamination by Salmonella spp. was 6.7% of carcasses (6/90) and 2.6% of carcass surface samples (7/270). All isolates were confirmed by PCR and qPCR. The serological analysis and the PFGE showed a single profile: Typhimurium. The strains demonstrated 100% susceptibility to ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamicin and tetracycline. Positive carcasses after cooling pose a direct risk to consumers, since the meat is considered ready to be marketed after this process.
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spelling Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil.Carcaça bovinaAbatedouro-frigoríficoSorotipoResistência a antibióticoCarne bovinaSalmonella sppCarcaçaSalmoneloseResfriamentoBovinoSlaughterhousesBrazilSerotypesAntibiotic resistanceSalmonellosisSkinningCoolingCattleThe aim of the present study was to investigate the presence of Salmonella spp. in samples collected from beef meat at three points of the slaughter line (after skinning, washing and cooling) at three slaughterhouses in Brazil that export meat. Detection was based on ISO 6579:2002 and confirmed by PCR and qPCR. The isolates were typified using slide agglutination tests and PFGE. The antibiotic sensitivity profile was determined using the disk diffusion method. Contamination was detected in only one slaughterhouse. The overall frequency of contamination by Salmonella spp. was 6.7% of carcasses (6/90) and 2.6% of carcass surface samples (7/270). All isolates were confirmed by PCR and qPCR. The serological analysis and the PFGE showed a single profile: Typhimurium. The strains demonstrated 100% susceptibility to ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamicin and tetracycline. Positive carcasses after cooling pose a direct risk to consumers, since the meat is considered ready to be marketed after this process.Título em português: Pesquisa de Salmonella spp. em carcaças bovinas durante o processamento em abatedouros‑frigoríficos exportadores.Daniele Bier, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; JALUSA DEON KICH, CNPSA; SABRINA CASTILHO DUARTE, CNPSA; MARCIO ROBERTO SILVA, CNPGL; Luiza M. Valsoni, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; Carlos A. N. Ramos, Faculdade de Medicina Veterinária e Zootecnia - FAMEZ/Universidade Federal de Mato Grosso do Sul - UFMS; Dália P. Rodrigues, Laboratório de Enterobactérias/Instituto Oswaldo Cruz; FLABIO RIBEIRO DE ARAUJO, CNPGC.BIER, D.KICH, J. D.DUARTE, S. C.SILVA, M. R.VALSONI, L. M.RAMOS, C. A. N.RODRIGUES, D. P.ARAUJO, F. R.2018-12-22T23:37:27Z2018-12-22T23:37:27Z2018-12-2020182018-12-22T23:37:27Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 11, p. 2037-2043, novembro, 2018http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102218enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-12-22T23:37:33Zoai:www.alice.cnptia.embrapa.br:doc/1102218Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-12-22T23:37:33falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-12-22T23:37:33Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil.
title Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil.
spellingShingle Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil.
BIER, D.
Carcaça bovina
Abatedouro-frigorífico
Sorotipo
Resistência a antibiótico
Carne bovina
Salmonella spp
Carcaça
Salmonelose
Resfriamento
Bovino
Slaughterhouses
Brazil
Serotypes
Antibiotic resistance
Salmonellosis
Skinning
Cooling
Cattle
title_short Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil.
title_full Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil.
title_fullStr Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil.
title_full_unstemmed Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil.
title_sort Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil.
author BIER, D.
author_facet BIER, D.
KICH, J. D.
DUARTE, S. C.
SILVA, M. R.
VALSONI, L. M.
RAMOS, C. A. N.
RODRIGUES, D. P.
ARAUJO, F. R.
author_role author
author2 KICH, J. D.
DUARTE, S. C.
SILVA, M. R.
VALSONI, L. M.
RAMOS, C. A. N.
RODRIGUES, D. P.
ARAUJO, F. R.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Daniele Bier, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; JALUSA DEON KICH, CNPSA; SABRINA CASTILHO DUARTE, CNPSA; MARCIO ROBERTO SILVA, CNPGL; Luiza M. Valsoni, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; Carlos A. N. Ramos, Faculdade de Medicina Veterinária e Zootecnia - FAMEZ/Universidade Federal de Mato Grosso do Sul - UFMS; Dália P. Rodrigues, Laboratório de Enterobactérias/Instituto Oswaldo Cruz; FLABIO RIBEIRO DE ARAUJO, CNPGC.
dc.contributor.author.fl_str_mv BIER, D.
KICH, J. D.
DUARTE, S. C.
SILVA, M. R.
VALSONI, L. M.
RAMOS, C. A. N.
RODRIGUES, D. P.
ARAUJO, F. R.
dc.subject.por.fl_str_mv Carcaça bovina
Abatedouro-frigorífico
Sorotipo
Resistência a antibiótico
Carne bovina
Salmonella spp
Carcaça
Salmonelose
Resfriamento
Bovino
Slaughterhouses
Brazil
Serotypes
Antibiotic resistance
Salmonellosis
Skinning
Cooling
Cattle
topic Carcaça bovina
Abatedouro-frigorífico
Sorotipo
Resistência a antibiótico
Carne bovina
Salmonella spp
Carcaça
Salmonelose
Resfriamento
Bovino
Slaughterhouses
Brazil
Serotypes
Antibiotic resistance
Salmonellosis
Skinning
Cooling
Cattle
description The aim of the present study was to investigate the presence of Salmonella spp. in samples collected from beef meat at three points of the slaughter line (after skinning, washing and cooling) at three slaughterhouses in Brazil that export meat. Detection was based on ISO 6579:2002 and confirmed by PCR and qPCR. The isolates were typified using slide agglutination tests and PFGE. The antibiotic sensitivity profile was determined using the disk diffusion method. Contamination was detected in only one slaughterhouse. The overall frequency of contamination by Salmonella spp. was 6.7% of carcasses (6/90) and 2.6% of carcass surface samples (7/270). All isolates were confirmed by PCR and qPCR. The serological analysis and the PFGE showed a single profile: Typhimurium. The strains demonstrated 100% susceptibility to ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamicin and tetracycline. Positive carcasses after cooling pose a direct risk to consumers, since the meat is considered ready to be marketed after this process.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-22T23:37:27Z
2018-12-22T23:37:27Z
2018-12-20
2018
2018-12-22T23:37:27Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 11, p. 2037-2043, novembro, 2018
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102218
identifier_str_mv Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 11, p. 2037-2043, novembro, 2018
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102218
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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