Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil).
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/861663 |
Resumo: | To identify DNA of the main tick-borne pathogens in dogs from Recife (Brazil), polymerase chain reactions were carried out on blood samples of dogs treated at the Veterinary Hospital of the Universidade Federal Rural de Pernambuco from March 2007 to June 2008. The detection of DNA was performed using specific primers. Amplicons were analyzed through electrophoresis and sequencing. A phylogenetic tree was constructed using the UPGMA method, revealing that the sequences were closely related to those of strains from other geographic regions. Among the 205 blood samples analyzed, 48.78% was positive for Anaplasma platys; 38.04% was positive for Ehrlichia canis; 7.31% was positive for Babesia canis vogeli; and 0.49% was positive for Hepatozoon canis and Mycoplasma haemocanis. Coinfection of two or three pathogens was found in 23.9% (49/205) of the dogs. The subspecies B. canis vogeli was identified. Infection by H. canis and M. haemocanis is reported for the first time in dogs in the state of Pernambuco (Brazil). The data indicate that the main tick-borne pathogens in dogs in this region are E. canis and/or A. platys, followed by B. canis vogeli. |
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Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil).Sanidade AnimalTo identify DNA of the main tick-borne pathogens in dogs from Recife (Brazil), polymerase chain reactions were carried out on blood samples of dogs treated at the Veterinary Hospital of the Universidade Federal Rural de Pernambuco from March 2007 to June 2008. The detection of DNA was performed using specific primers. Amplicons were analyzed through electrophoresis and sequencing. A phylogenetic tree was constructed using the UPGMA method, revealing that the sequences were closely related to those of strains from other geographic regions. Among the 205 blood samples analyzed, 48.78% was positive for Anaplasma platys; 38.04% was positive for Ehrlichia canis; 7.31% was positive for Babesia canis vogeli; and 0.49% was positive for Hepatozoon canis and Mycoplasma haemocanis. Coinfection of two or three pathogens was found in 23.9% (49/205) of the dogs. The subspecies B. canis vogeli was identified. Infection by H. canis and M. haemocanis is reported for the first time in dogs in the state of Pernambuco (Brazil). The data indicate that the main tick-borne pathogens in dogs in this region are E. canis and/or A. platys, followed by B. canis vogeli.RAFAEL RAMOS, UFRPE; CARLOS RAMOS, UFRPE; FLABIO RIBEIRO ARAUJO, CNPGC; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; INGRID SOUZA, BOLSISTA CNPGC; DANILLO PIMENTEL, UFRPE; MARIANA GALINDO, UFRPE; MARILIA SANTANA, UFRPE; EDUARDO ROSAS, UFRPE; MARIA FAUSTINO, UFRPE; LEUCIO ALVES, UFRPE.RAMOS, R.RAMOS, C.ARAUJO, F. R.OLIVEIRA, R. H. M. deSOUZA, I.PIMENTEL, D.GALINDO, M.SANTAN, M.ROSAS, E.FAUSTINO, M.ALVES, L.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2010-09-0220102016-10-13T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleParasitology Research, v.107, n. 5, p.1115-1120, Oct. 2010.http://www.alice.cnptia.embrapa.br/alice/handle/doc/86166310.1007/s00436-010-1979-7porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:41:06Zoai:www.alice.cnptia.embrapa.br:doc/861663Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:41:06falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:41:06Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). |
title |
Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). |
spellingShingle |
Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). RAMOS, R. Sanidade Animal |
title_short |
Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). |
title_full |
Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). |
title_fullStr |
Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). |
title_full_unstemmed |
Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). |
title_sort |
Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). |
author |
RAMOS, R. |
author_facet |
RAMOS, R. RAMOS, C. ARAUJO, F. R. OLIVEIRA, R. H. M. de SOUZA, I. PIMENTEL, D. GALINDO, M. SANTAN, M. ROSAS, E. FAUSTINO, M. ALVES, L. |
author_role |
author |
author2 |
RAMOS, C. ARAUJO, F. R. OLIVEIRA, R. H. M. de SOUZA, I. PIMENTEL, D. GALINDO, M. SANTAN, M. ROSAS, E. FAUSTINO, M. ALVES, L. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
RAFAEL RAMOS, UFRPE; CARLOS RAMOS, UFRPE; FLABIO RIBEIRO ARAUJO, CNPGC; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; INGRID SOUZA, BOLSISTA CNPGC; DANILLO PIMENTEL, UFRPE; MARIANA GALINDO, UFRPE; MARILIA SANTANA, UFRPE; EDUARDO ROSAS, UFRPE; MARIA FAUSTINO, UFRPE; LEUCIO ALVES, UFRPE. |
dc.contributor.author.fl_str_mv |
RAMOS, R. RAMOS, C. ARAUJO, F. R. OLIVEIRA, R. H. M. de SOUZA, I. PIMENTEL, D. GALINDO, M. SANTAN, M. ROSAS, E. FAUSTINO, M. ALVES, L. |
dc.subject.por.fl_str_mv |
Sanidade Animal |
topic |
Sanidade Animal |
description |
To identify DNA of the main tick-borne pathogens in dogs from Recife (Brazil), polymerase chain reactions were carried out on blood samples of dogs treated at the Veterinary Hospital of the Universidade Federal Rural de Pernambuco from March 2007 to June 2008. The detection of DNA was performed using specific primers. Amplicons were analyzed through electrophoresis and sequencing. A phylogenetic tree was constructed using the UPGMA method, revealing that the sequences were closely related to those of strains from other geographic regions. Among the 205 blood samples analyzed, 48.78% was positive for Anaplasma platys; 38.04% was positive for Ehrlichia canis; 7.31% was positive for Babesia canis vogeli; and 0.49% was positive for Hepatozoon canis and Mycoplasma haemocanis. Coinfection of two or three pathogens was found in 23.9% (49/205) of the dogs. The subspecies B. canis vogeli was identified. Infection by H. canis and M. haemocanis is reported for the first time in dogs in the state of Pernambuco (Brazil). The data indicate that the main tick-borne pathogens in dogs in this region are E. canis and/or A. platys, followed by B. canis vogeli. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-09-02 2010 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2016-10-13T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Parasitology Research, v.107, n. 5, p.1115-1120, Oct. 2010. http://www.alice.cnptia.embrapa.br/alice/handle/doc/861663 10.1007/s00436-010-1979-7 |
identifier_str_mv |
Parasitology Research, v.107, n. 5, p.1115-1120, Oct. 2010. 10.1007/s00436-010-1979-7 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/861663 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503426567045120 |