Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.

Detalhes bibliográficos
Autor(a) principal: SILVA, G. A. da
Data de Publicação: 2012
Outros Autores: BERNARDI, T. L., SCHAKER, P. D. C., MENEGOTTO, M., VALENTE, P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/925175
Resumo: The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.
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spelling Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.Gelo-degeloEbuliçãoExtração de DNAMetodologiaMicrobiologiaDNASaccharomyces CerevisiaeDekkera bruxellensisThe purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.GILDO ALMEIDA DA SILVA, CNPUV; Taís Letícia Bernardi, CNPUV (BOLSISTA); Patrícia Dayane Carvalho Schaker, CNPUV (BOLSISTA); Morgana Menegotto, CNPUV (BOLSISTA); Patricia Valente, UFRGS.SILVA, G. A. daBERNARDI, T. L.SCHAKER, P. D. C.MENEGOTTO, M.VALENTE, P.2012-05-22T11:11:11Z2012-05-22T11:11:11Z2012-05-22T11:11:11Z2012-05-22T11:11:11Z2012-05-2220122012-05-22T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Archives of Biology and Technology, Curitiba, v. 55, n. 2, p. 319-327, mar./abr. 2012.http://www.alice.cnptia.embrapa.br/alice/handle/doc/925175enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:46:15Zoai:www.alice.cnptia.embrapa.br:doc/925175Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:46:15falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:46:15Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.
title Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.
spellingShingle Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.
SILVA, G. A. da
Gelo-degelo
Ebulição
Extração de DNA
Metodologia
Microbiologia
DNA
Saccharomyces Cerevisiae
Dekkera bruxellensis
title_short Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.
title_full Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.
title_fullStr Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.
title_full_unstemmed Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.
title_sort Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.
author SILVA, G. A. da
author_facet SILVA, G. A. da
BERNARDI, T. L.
SCHAKER, P. D. C.
MENEGOTTO, M.
VALENTE, P.
author_role author
author2 BERNARDI, T. L.
SCHAKER, P. D. C.
MENEGOTTO, M.
VALENTE, P.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv GILDO ALMEIDA DA SILVA, CNPUV; Taís Letícia Bernardi, CNPUV (BOLSISTA); Patrícia Dayane Carvalho Schaker, CNPUV (BOLSISTA); Morgana Menegotto, CNPUV (BOLSISTA); Patricia Valente, UFRGS.
dc.contributor.author.fl_str_mv SILVA, G. A. da
BERNARDI, T. L.
SCHAKER, P. D. C.
MENEGOTTO, M.
VALENTE, P.
dc.subject.por.fl_str_mv Gelo-degelo
Ebulição
Extração de DNA
Metodologia
Microbiologia
DNA
Saccharomyces Cerevisiae
Dekkera bruxellensis
topic Gelo-degelo
Ebulição
Extração de DNA
Metodologia
Microbiologia
DNA
Saccharomyces Cerevisiae
Dekkera bruxellensis
description The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.
publishDate 2012
dc.date.none.fl_str_mv 2012-05-22T11:11:11Z
2012-05-22T11:11:11Z
2012-05-22T11:11:11Z
2012-05-22T11:11:11Z
2012-05-22
2012
2012-05-22T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Brazilian Archives of Biology and Technology, Curitiba, v. 55, n. 2, p. 319-327, mar./abr. 2012.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/925175
identifier_str_mv Brazilian Archives of Biology and Technology, Curitiba, v. 55, n. 2, p. 319-327, mar./abr. 2012.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/925175
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
_version_ 1794503363416555520

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