Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification

Detalhes bibliográficos
Autor(a) principal: Silva,Gildo Almeida da
Data de Publicação: 2012
Outros Autores: Bernardi,Taís Letícia, Schaker,Patrícia Dayane Carvalho, Menegotto,Morgana, Valente,Patricia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200020
Resumo: The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.
id TECPAR-1_39404e863f4203430d0b6c703bb72091
oai_identifier_str oai:scielo:S1516-89132012000200020
network_acronym_str TECPAR-1
network_name_str Brazilian Archives of Biology and Technology
repository_id_str
spelling Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purificationBoilingfreeze-thawingDNA extractionDekkera bruxellensisSaccharomyces cerevisiaeThe purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.Instituto de Tecnologia do Paraná - Tecpar2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200020Brazilian Archives of Biology and Technology v.55 n.2 2012reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132012000200020info:eu-repo/semantics/openAccessSilva,Gildo Almeida daBernardi,Taís LetíciaSchaker,Patrícia Dayane CarvalhoMenegotto,MorganaValente,Patriciaeng2012-05-03T00:00:00Zoai:scielo:S1516-89132012000200020Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2012-05-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification
title Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification
spellingShingle Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification
Silva,Gildo Almeida da
Boiling
freeze-thawing
DNA extraction
Dekkera bruxellensis
Saccharomyces cerevisiae
title_short Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification
title_full Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification
title_fullStr Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification
title_full_unstemmed Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification
title_sort Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification
author Silva,Gildo Almeida da
author_facet Silva,Gildo Almeida da
Bernardi,Taís Letícia
Schaker,Patrícia Dayane Carvalho
Menegotto,Morgana
Valente,Patricia
author_role author
author2 Bernardi,Taís Letícia
Schaker,Patrícia Dayane Carvalho
Menegotto,Morgana
Valente,Patricia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva,Gildo Almeida da
Bernardi,Taís Letícia
Schaker,Patrícia Dayane Carvalho
Menegotto,Morgana
Valente,Patricia
dc.subject.por.fl_str_mv Boiling
freeze-thawing
DNA extraction
Dekkera bruxellensis
Saccharomyces cerevisiae
topic Boiling
freeze-thawing
DNA extraction
Dekkera bruxellensis
Saccharomyces cerevisiae
description The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132012000200020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.55 n.2 2012
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318275198189568

Registros relacionados