Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.

Detalhes bibliográficos
Autor(a) principal: RUIZ, J. C.
Data de Publicação: 2011
Outros Autores: D'AFONSECA, V., SILVA, A., ALI, A., PINTO, A. C., SANTOS, A. R., ROCHA, A. A. M. C., LOPES, D. O., DORELLA, F. A., PACHECO, L. G. C., COSTA, M. P., TURK, M. Z., SEYFFERT, N., MORAES, P. M. R. O., SOARES, S. C., ALMEIDA, S. S., CASTRO, T. L. P., ABREU, V. A. C., TROST, E., BAUMBACH, J., TAUCH, A., SCHNEIDER, M. P. C., McCULLOCH, J., CERDEIRA, L. T., RAMOS, R. T. J., ZERLOTINI, A., DOMINITINI, A., RESENDE, D. M., COSER, E. M., OLIVEIRA, L. M., PEDROSA, A. L., VIEIRA, C. U., GUIMARAES, C. T., BARTHOLOMEU, D. C., OLIVEIRA, D. M., SANTOS, F. R., RABELO, E. M., LOBO, F. P., FRANCO, G. R., COSTA, A. F., CASTRO, I. M., DIAS, S. R. C., FERRO, J. A., ORTEGA, J. M., PAIVA, L. V., ALMEIDA, J. F., GOULART, L. R., FERRO, M. I. T., CARNEIRO, N. P., FALCÃO, P. R. K., GRYNBERG, P., TEIXEIRA, S. M. R., BROMMONSCHENKEL, S., OLIVEIRA, S. C., MEYER, R., MOORE, R. J., MIYOSHI, A., OLIVEIRA, G. C., AZEVEDO, V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/906799
Resumo: Background: Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity. Methodology and Findings: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal transfer. Conclusions: These particular genome characteristics of C. pseudotuberculosis, as well as its acquired virulence factors in pathogenicity islands, provide evidence of its lifestyle and of the pathogenicity pathways used by this pathogen in the infection process. All genomes cited in this study are available in the NCBI Genbank database (http://www.ncbi.nlm.nih.gov/genbank/) under accession numbers CP001809 and CP001829.
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spelling Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.GenéticaPatógenoGenomeBackground: Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity. Methodology and Findings: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal transfer. Conclusions: These particular genome characteristics of C. pseudotuberculosis, as well as its acquired virulence factors in pathogenicity islands, provide evidence of its lifestyle and of the pathogenicity pathways used by this pathogen in the infection process. All genomes cited in this study are available in the NCBI Genbank database (http://www.ncbi.nlm.nih.gov/genbank/) under accession numbers CP001809 and CP001829.CLAUDIA TEIXEIRA GUIMARAES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS.RUIZ, J. C.D'AFONSECA, V.SILVA, A.ALI, A.PINTO, A. C.SANTOS, A. R.ROCHA, A. A. M. C.LOPES, D. O.DORELLA, F. A.PACHECO, L. G. C.COSTA, M. P.TURK, M. Z.SEYFFERT, N.MORAES, P. M. R. O.SOARES, S. C.ALMEIDA, S. S.CASTRO, T. L. P.ABREU, V. A. C.TROST, E.BAUMBACH, J.TAUCH, A.SCHNEIDER, M. P. C.McCULLOCH, J.CERDEIRA, L. T.RAMOS, R. T. J.ZERLOTINI, A.DOMINITINI, A.RESENDE, D. M.COSER, E. M.OLIVEIRA, L. M.PEDROSA, A. L.VIEIRA, C. U.GUIMARAES, C. T.BARTHOLOMEU, D. C.OLIVEIRA, D. M.SANTOS, F. R.RABELO, E. M.LOBO, F. P.FRANCO, G. R.COSTA, A. F.CASTRO, I. M.DIAS, S. R. C.FERRO, J. A.ORTEGA, J. M.PAIVA, L. V.ALMEIDA, J. F.GOULART, L. R.FERRO, M. I. T.CARNEIRO, N. P.FALCÃO, P. R. K.GRYNBERG, P.TEIXEIRA, S. M. R.BROMMONSCHENKEL, S.OLIVEIRA, S. C.MEYER, R.MOORE, R. J.MIYOSHI, A.OLIVEIRA, G. C.AZEVEDO, V.2011-11-23T11:11:11Z2011-11-23T11:11:11Z2011-11-2320112017-09-28T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, San Francisco, v. 6, n. 4, p. 1-16, 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/90679910.1371/journal.pone.0018551enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:20:48Zoai:www.alice.cnptia.embrapa.br:doc/906799Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:20:48falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:20:48Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.
title Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.
spellingShingle Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.
RUIZ, J. C.
Genética
Patógeno
Genome
title_short Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.
title_full Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.
title_fullStr Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.
title_full_unstemmed Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.
title_sort Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.
author RUIZ, J. C.
author_facet RUIZ, J. C.
D'AFONSECA, V.
SILVA, A.
ALI, A.
PINTO, A. C.
SANTOS, A. R.
ROCHA, A. A. M. C.
LOPES, D. O.
DORELLA, F. A.
PACHECO, L. G. C.
COSTA, M. P.
TURK, M. Z.
SEYFFERT, N.
MORAES, P. M. R. O.
SOARES, S. C.
ALMEIDA, S. S.
CASTRO, T. L. P.
ABREU, V. A. C.
TROST, E.
BAUMBACH, J.
TAUCH, A.
SCHNEIDER, M. P. C.
McCULLOCH, J.
CERDEIRA, L. T.
RAMOS, R. T. J.
ZERLOTINI, A.
DOMINITINI, A.
RESENDE, D. M.
COSER, E. M.
OLIVEIRA, L. M.
PEDROSA, A. L.
VIEIRA, C. U.
GUIMARAES, C. T.
BARTHOLOMEU, D. C.
OLIVEIRA, D. M.
SANTOS, F. R.
RABELO, E. M.
LOBO, F. P.
FRANCO, G. R.
COSTA, A. F.
CASTRO, I. M.
DIAS, S. R. C.
FERRO, J. A.
ORTEGA, J. M.
PAIVA, L. V.
ALMEIDA, J. F.
GOULART, L. R.
FERRO, M. I. T.
CARNEIRO, N. P.
FALCÃO, P. R. K.
GRYNBERG, P.
TEIXEIRA, S. M. R.
BROMMONSCHENKEL, S.
OLIVEIRA, S. C.
MEYER, R.
MOORE, R. J.
MIYOSHI, A.
OLIVEIRA, G. C.
AZEVEDO, V.
author_role author
author2 D'AFONSECA, V.
SILVA, A.
ALI, A.
PINTO, A. C.
SANTOS, A. R.
ROCHA, A. A. M. C.
LOPES, D. O.
DORELLA, F. A.
PACHECO, L. G. C.
COSTA, M. P.
TURK, M. Z.
SEYFFERT, N.
MORAES, P. M. R. O.
SOARES, S. C.
ALMEIDA, S. S.
CASTRO, T. L. P.
ABREU, V. A. C.
TROST, E.
BAUMBACH, J.
TAUCH, A.
SCHNEIDER, M. P. C.
McCULLOCH, J.
CERDEIRA, L. T.
RAMOS, R. T. J.
ZERLOTINI, A.
DOMINITINI, A.
RESENDE, D. M.
COSER, E. M.
OLIVEIRA, L. M.
PEDROSA, A. L.
VIEIRA, C. U.
GUIMARAES, C. T.
BARTHOLOMEU, D. C.
OLIVEIRA, D. M.
SANTOS, F. R.
RABELO, E. M.
LOBO, F. P.
FRANCO, G. R.
COSTA, A. F.
CASTRO, I. M.
DIAS, S. R. C.
FERRO, J. A.
ORTEGA, J. M.
PAIVA, L. V.
ALMEIDA, J. F.
GOULART, L. R.
FERRO, M. I. T.
CARNEIRO, N. P.
FALCÃO, P. R. K.
GRYNBERG, P.
TEIXEIRA, S. M. R.
BROMMONSCHENKEL, S.
OLIVEIRA, S. C.
MEYER, R.
MOORE, R. J.
MIYOSHI, A.
OLIVEIRA, G. C.
AZEVEDO, V.
author2_role author
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author
author
author
author
author
author
author
author
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author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
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author
dc.contributor.none.fl_str_mv CLAUDIA TEIXEIRA GUIMARAES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS.
dc.contributor.author.fl_str_mv RUIZ, J. C.
D'AFONSECA, V.
SILVA, A.
ALI, A.
PINTO, A. C.
SANTOS, A. R.
ROCHA, A. A. M. C.
LOPES, D. O.
DORELLA, F. A.
PACHECO, L. G. C.
COSTA, M. P.
TURK, M. Z.
SEYFFERT, N.
MORAES, P. M. R. O.
SOARES, S. C.
ALMEIDA, S. S.
CASTRO, T. L. P.
ABREU, V. A. C.
TROST, E.
BAUMBACH, J.
TAUCH, A.
SCHNEIDER, M. P. C.
McCULLOCH, J.
CERDEIRA, L. T.
RAMOS, R. T. J.
ZERLOTINI, A.
DOMINITINI, A.
RESENDE, D. M.
COSER, E. M.
OLIVEIRA, L. M.
PEDROSA, A. L.
VIEIRA, C. U.
GUIMARAES, C. T.
BARTHOLOMEU, D. C.
OLIVEIRA, D. M.
SANTOS, F. R.
RABELO, E. M.
LOBO, F. P.
FRANCO, G. R.
COSTA, A. F.
CASTRO, I. M.
DIAS, S. R. C.
FERRO, J. A.
ORTEGA, J. M.
PAIVA, L. V.
ALMEIDA, J. F.
GOULART, L. R.
FERRO, M. I. T.
CARNEIRO, N. P.
FALCÃO, P. R. K.
GRYNBERG, P.
TEIXEIRA, S. M. R.
BROMMONSCHENKEL, S.
OLIVEIRA, S. C.
MEYER, R.
MOORE, R. J.
MIYOSHI, A.
OLIVEIRA, G. C.
AZEVEDO, V.
dc.subject.por.fl_str_mv Genética
Patógeno
Genome
topic Genética
Patógeno
Genome
description Background: Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity. Methodology and Findings: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal transfer. Conclusions: These particular genome characteristics of C. pseudotuberculosis, as well as its acquired virulence factors in pathogenicity islands, provide evidence of its lifestyle and of the pathogenicity pathways used by this pathogen in the infection process. All genomes cited in this study are available in the NCBI Genbank database (http://www.ncbi.nlm.nih.gov/genbank/) under accession numbers CP001809 and CP001829.
publishDate 2011
dc.date.none.fl_str_mv 2011-11-23T11:11:11Z
2011-11-23T11:11:11Z
2011-11-23
2011
2017-09-28T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Plos One, San Francisco, v. 6, n. 4, p. 1-16, 2011.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/906799
10.1371/journal.pone.0018551
identifier_str_mv Plos One, San Francisco, v. 6, n. 4, p. 1-16, 2011.
10.1371/journal.pone.0018551
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/906799
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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