Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1071838 |
Resumo: | The hemibiotrophic fungus Colletotrichum graminicola may cause severe damage to maize, affecting normal development of the plant and decreasing grain yield. In this context, understanding plant defense pathways at the inoculation site and systemically in uninoculated tissues can help in the development of genetic engineering of resistance against this pathogen. Previous work has discussed the molecular basis of maize - C. graminicola interaction. However, many genes involved in defense have not yet been exploited for lack of annotation in public databases. Here, changes in global gene expression were studied in root, male and female inflorescences of maize under local and systemic fungal infection treatments, respectively. RNA-Seq with qPCR was used to indicate genes involved in plant defense. We found that systemic acquired resistance induction in female inflorescences mainly involves accumulation of salicylic acid (SA)-inducible defense genes (ZmNAC, ZmHSF, ZmWRKY, ZmbZIP and PR1) and potential genes involved in chromatin modification. Furthermore, transcripts involved in jasmonic acid (JA) and ethylene (ET) signaling pathways were also accumulated and may participate in plant immunity. Moreover, several genes were functionally re-annotated based on domain signature, indicating novel candidates to be tested in strategies involving gene knockout and overexpression in plants. |
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Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize.Resistência a doençaDoença de plantaMilhoAntracnoseGeneThe hemibiotrophic fungus Colletotrichum graminicola may cause severe damage to maize, affecting normal development of the plant and decreasing grain yield. In this context, understanding plant defense pathways at the inoculation site and systemically in uninoculated tissues can help in the development of genetic engineering of resistance against this pathogen. Previous work has discussed the molecular basis of maize - C. graminicola interaction. However, many genes involved in defense have not yet been exploited for lack of annotation in public databases. Here, changes in global gene expression were studied in root, male and female inflorescences of maize under local and systemic fungal infection treatments, respectively. RNA-Seq with qPCR was used to indicate genes involved in plant defense. We found that systemic acquired resistance induction in female inflorescences mainly involves accumulation of salicylic acid (SA)-inducible defense genes (ZmNAC, ZmHSF, ZmWRKY, ZmbZIP and PR1) and potential genes involved in chromatin modification. Furthermore, transcripts involved in jasmonic acid (JA) and ethylene (ET) signaling pathways were also accumulated and may participate in plant immunity. Moreover, several genes were functionally re-annotated based on domain signature, indicating novel candidates to be tested in strategies involving gene knockout and overexpression in plants.LUCIANO VIANA COTA, CNPMS.MIRANDA, V. de J.PORTO, W. F.FERNANDES, G. da R.POGUE, R.NOLASCO, D. O.ARAUJO, A. C. G.COTA, L. V.FREITAS, C. G. deDIAS, S. C.FRANCO, O. L.2017-07-03T11:11:11Z2017-07-03T11:11:11Z2017-07-0320172017-07-03T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleScientific Reports, v. 7, n. 2483, p. 1-14, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/107183810.1038/s41598-017-02298-8enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:33:58Zoai:www.alice.cnptia.embrapa.br:doc/1071838Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:33:58falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:33:58Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize. |
title |
Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize. |
spellingShingle |
Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize. MIRANDA, V. de J. Resistência a doença Doença de planta Milho Antracnose Gene |
title_short |
Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize. |
title_full |
Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize. |
title_fullStr |
Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize. |
title_full_unstemmed |
Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize. |
title_sort |
Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize. |
author |
MIRANDA, V. de J. |
author_facet |
MIRANDA, V. de J. PORTO, W. F. FERNANDES, G. da R. POGUE, R. NOLASCO, D. O. ARAUJO, A. C. G. COTA, L. V. FREITAS, C. G. de DIAS, S. C. FRANCO, O. L. |
author_role |
author |
author2 |
PORTO, W. F. FERNANDES, G. da R. POGUE, R. NOLASCO, D. O. ARAUJO, A. C. G. COTA, L. V. FREITAS, C. G. de DIAS, S. C. FRANCO, O. L. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
LUCIANO VIANA COTA, CNPMS. |
dc.contributor.author.fl_str_mv |
MIRANDA, V. de J. PORTO, W. F. FERNANDES, G. da R. POGUE, R. NOLASCO, D. O. ARAUJO, A. C. G. COTA, L. V. FREITAS, C. G. de DIAS, S. C. FRANCO, O. L. |
dc.subject.por.fl_str_mv |
Resistência a doença Doença de planta Milho Antracnose Gene |
topic |
Resistência a doença Doença de planta Milho Antracnose Gene |
description |
The hemibiotrophic fungus Colletotrichum graminicola may cause severe damage to maize, affecting normal development of the plant and decreasing grain yield. In this context, understanding plant defense pathways at the inoculation site and systemically in uninoculated tissues can help in the development of genetic engineering of resistance against this pathogen. Previous work has discussed the molecular basis of maize - C. graminicola interaction. However, many genes involved in defense have not yet been exploited for lack of annotation in public databases. Here, changes in global gene expression were studied in root, male and female inflorescences of maize under local and systemic fungal infection treatments, respectively. RNA-Seq with qPCR was used to indicate genes involved in plant defense. We found that systemic acquired resistance induction in female inflorescences mainly involves accumulation of salicylic acid (SA)-inducible defense genes (ZmNAC, ZmHSF, ZmWRKY, ZmbZIP and PR1) and potential genes involved in chromatin modification. Furthermore, transcripts involved in jasmonic acid (JA) and ethylene (ET) signaling pathways were also accumulated and may participate in plant immunity. Moreover, several genes were functionally re-annotated based on domain signature, indicating novel candidates to be tested in strategies involving gene knockout and overexpression in plants. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-03T11:11:11Z 2017-07-03T11:11:11Z 2017-07-03 2017 2017-07-03T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Scientific Reports, v. 7, n. 2483, p. 1-14, 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1071838 10.1038/s41598-017-02298-8 |
identifier_str_mv |
Scientific Reports, v. 7, n. 2483, p. 1-14, 2017. 10.1038/s41598-017-02298-8 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1071838 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503438683340800 |