Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado.

• Autor(a) principal: FARIA, J. C.
• Data de Publicação: 2001
• Outros Autores: ALBINO, M. M. C., DIAS, B. B. A., VIANNA, M. G. R., ARAGÃO, F. J. L.
• Idioma: por
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/209188
• topic: BGMV; Tolerância; Biobalística; Transformação; Feijão; Mosaico Dourado; Phaseolus Vulgaris; Vírus; Bean golden mosaic virus; mutation

Tbe rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication, and rep gene rnutations with amino acid codons cbanges in the putative NTP-binding rnotif D262R (MI) was created. Phaseolus vulgaris rransforrnation was achieved with a helium driven particle acceleration device. Tbe vector contained the bar gene [resistance to arnmonium glyphosinate (AG)] and besides the rep gene mutant. A total of J 7 initial - R(O) - transformants were analyzed. From the R(O) plant M1/4, which had mutation MI, six progeny plants with resistance to AG were obtained, and four remained BGMV symptomless after inoculation. From tbese four plants, a total progeny of 127 R(2) plants were inoculated. Of the 35 progenies of plant M 1/4/l, only three plants showed syrnptoms. From the other 92 plants, 72 had symptoms. Two plants out of 20 in the nontransgenic control plants remained symptornless.