Somatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves.
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/137733 |
Resumo: | The regeneration and cytogenetic analysis of 7 cassava (Manihot esculenta Crantz) cultivars obtained by organogenesis and somatic embryogenesis were investigated. In the first phase, callus formation was induced in young leaves using MS medium supplemented with 1.0, 5.0, 10.0, 15.0 and 20.0 mg/l of 2,4-D or without a growth regulator (control). In the second phase (development), the calli obtained in the above treatments were transferred to MS medium supplemented with factorial combinations of GA3 and BAP at 0, 0.5, 1.0, 1.5 and 2.0 mg/l. Results of the first phase showed that 2,4-D induced the formation of both friable and compact calli. The compact calli displayed root and shoot formation, whilst somatic embryos were obtained only from friable calli in the medium with 1.0 mg/l GA3. Plants obtained from somatic embryos were regenerated in MS medium supplemented with 0.04 mg/l NAA and 0.05 mg/l GA3. Cytogenetic analysis in 167 regenerated plants from somatic embryogenesis and 176 from organogenesis showed 2n= 36 as a normal diploid chromosome number. |
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Somatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves.AnáliseCaloCitogenética VegetalEmbriogéneseMandiocaManihot EsculentaMicropropagaçãoVariedadeThe regeneration and cytogenetic analysis of 7 cassava (Manihot esculenta Crantz) cultivars obtained by organogenesis and somatic embryogenesis were investigated. In the first phase, callus formation was induced in young leaves using MS medium supplemented with 1.0, 5.0, 10.0, 15.0 and 20.0 mg/l of 2,4-D or without a growth regulator (control). In the second phase (development), the calli obtained in the above treatments were transferred to MS medium supplemented with factorial combinations of GA3 and BAP at 0, 0.5, 1.0, 1.5 and 2.0 mg/l. Results of the first phase showed that 2,4-D induced the formation of both friable and compact calli. The compact calli displayed root and shoot formation, whilst somatic embryos were obtained only from friable calli in the medium with 1.0 mg/l GA3. Plants obtained from somatic embryos were regenerated in MS medium supplemented with 0.04 mg/l NAA and 0.05 mg/l GA3. Cytogenetic analysis in 167 regenerated plants from somatic embryogenesis and 176 from organogenesis showed 2n= 36 as a normal diploid chromosome number.NATONIEL FRANKLIN DE MELO, CPATSA.MELO, N. F. de2018-05-15T00:33:28Z2018-05-15T00:33:28Z2003-03-1420022018-05-15T00:33:28Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCytologia, Tpkyo, v. 67, n. 4, p. 337-341, 2002.http://www.alice.cnptia.embrapa.br/alice/handle/doc/137733enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-05-15T00:33:34Zoai:www.alice.cnptia.embrapa.br:doc/137733Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-05-15T00:33:34falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-05-15T00:33:34Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Somatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves. |
title |
Somatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves. |
spellingShingle |
Somatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves. MELO, N. F. de Análise Calo Citogenética Vegetal Embriogénese Mandioca Manihot Esculenta Micropropagação Variedade |
title_short |
Somatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves. |
title_full |
Somatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves. |
title_fullStr |
Somatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves. |
title_full_unstemmed |
Somatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves. |
title_sort |
Somatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves. |
author |
MELO, N. F. de |
author_facet |
MELO, N. F. de |
author_role |
author |
dc.contributor.none.fl_str_mv |
NATONIEL FRANKLIN DE MELO, CPATSA. |
dc.contributor.author.fl_str_mv |
MELO, N. F. de |
dc.subject.por.fl_str_mv |
Análise Calo Citogenética Vegetal Embriogénese Mandioca Manihot Esculenta Micropropagação Variedade |
topic |
Análise Calo Citogenética Vegetal Embriogénese Mandioca Manihot Esculenta Micropropagação Variedade |
description |
The regeneration and cytogenetic analysis of 7 cassava (Manihot esculenta Crantz) cultivars obtained by organogenesis and somatic embryogenesis were investigated. In the first phase, callus formation was induced in young leaves using MS medium supplemented with 1.0, 5.0, 10.0, 15.0 and 20.0 mg/l of 2,4-D or without a growth regulator (control). In the second phase (development), the calli obtained in the above treatments were transferred to MS medium supplemented with factorial combinations of GA3 and BAP at 0, 0.5, 1.0, 1.5 and 2.0 mg/l. Results of the first phase showed that 2,4-D induced the formation of both friable and compact calli. The compact calli displayed root and shoot formation, whilst somatic embryos were obtained only from friable calli in the medium with 1.0 mg/l GA3. Plants obtained from somatic embryos were regenerated in MS medium supplemented with 0.04 mg/l NAA and 0.05 mg/l GA3. Cytogenetic analysis in 167 regenerated plants from somatic embryogenesis and 176 from organogenesis showed 2n= 36 as a normal diploid chromosome number. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002 2003-03-14 2018-05-15T00:33:28Z 2018-05-15T00:33:28Z 2018-05-15T00:33:28Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Cytologia, Tpkyo, v. 67, n. 4, p. 337-341, 2002. http://www.alice.cnptia.embrapa.br/alice/handle/doc/137733 |
identifier_str_mv |
Cytologia, Tpkyo, v. 67, n. 4, p. 337-341, 2002. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/137733 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503453996744704 |