Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/950734 |
Resumo: | The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. |
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Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender.Cultura do embriãoSexagemGadoThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression.GRAZIELI MARINHEIRO MACHADO, UnB; ESTER SIQUEIRA CAIXETA, UnB; CAROLINA MADEIRA LUCCI, UnB; RODOLFO RUMPF, CENARGEN; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN.MACHADO, G. M.CAIXETA, E. S.LUCCI, C. M.RUMPF, R.FRANCO, M. M.DODE, M. A. N.2018-07-03T01:21:55Z2018-07-03T01:21:55Z2013-02-2220112018-07-03T01:21:55Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleZygote, v. 20, p. 123-134, 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/950734enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-07-03T01:22:01Zoai:www.alice.cnptia.embrapa.br:doc/950734Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-07-03T01:22:01falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-07-03T01:22:01Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
title |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
spellingShingle |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. MACHADO, G. M. Cultura do embrião Sexagem Gado |
title_short |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
title_full |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
title_fullStr |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
title_full_unstemmed |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
title_sort |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
author |
MACHADO, G. M. |
author_facet |
MACHADO, G. M. CAIXETA, E. S. LUCCI, C. M. RUMPF, R. FRANCO, M. M. DODE, M. A. N. |
author_role |
author |
author2 |
CAIXETA, E. S. LUCCI, C. M. RUMPF, R. FRANCO, M. M. DODE, M. A. N. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
GRAZIELI MARINHEIRO MACHADO, UnB; ESTER SIQUEIRA CAIXETA, UnB; CAROLINA MADEIRA LUCCI, UnB; RODOLFO RUMPF, CENARGEN; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
dc.contributor.author.fl_str_mv |
MACHADO, G. M. CAIXETA, E. S. LUCCI, C. M. RUMPF, R. FRANCO, M. M. DODE, M. A. N. |
dc.subject.por.fl_str_mv |
Cultura do embrião Sexagem Gado |
topic |
Cultura do embrião Sexagem Gado |
description |
The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2013-02-22 2018-07-03T01:21:55Z 2018-07-03T01:21:55Z 2018-07-03T01:21:55Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Zygote, v. 20, p. 123-134, 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/950734 |
identifier_str_mv |
Zygote, v. 20, p. 123-134, 2011. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/950734 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503458084093952 |