Molecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH).
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/491685 http://dx.doi.org/10.18512/1980-6477/rbms.v7n3p195-209 |
Resumo: | Bacillus thuringiensis (Berliner) strains 344 and 1644 belonging to the CNPMS/Embrapa (Maize and Sorghum National Research Center) Microorganism Bank, located in Sete Lagoas, MG, showed to be highly efficient in controlling fall armyworm, Spodotpera frugiperda (J.E. Smith). Strains 344 and 1644 were isolated from soil samples originated from Iguassu Falls and Rolândia, respectively, Paraná State, Brazil. While strain 344 harbors cry1Ab, cry1B, cry1E and cry1Fb genes, strain 1644 showed cry1B, cry1C, and cry1D and cry1Fb genes. The strain 344 crystal has bipyramidal shape and showed LC50 equal to 8.21 x 106 spores/mL. Strain 1644 has cuboid crystal and showed LC50 equal to 2.07 x 106 spores/mL. Comparative analysis of protein profile, plasmid and amplification with primers ERIC allowed the differentiation of the evaluated strains. |
id |
EMBR_dc2569a0a4bb91fa14d8144f5ab6524d |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/491685 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Molecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH).Cry genesBacillus Thuringiensisinsect pathologyBacillus thuringiensis (Berliner) strains 344 and 1644 belonging to the CNPMS/Embrapa (Maize and Sorghum National Research Center) Microorganism Bank, located in Sete Lagoas, MG, showed to be highly efficient in controlling fall armyworm, Spodotpera frugiperda (J.E. Smith). Strains 344 and 1644 were isolated from soil samples originated from Iguassu Falls and Rolândia, respectively, Paraná State, Brazil. While strain 344 harbors cry1Ab, cry1B, cry1E and cry1Fb genes, strain 1644 showed cry1B, cry1C, and cry1D and cry1Fb genes. The strain 344 crystal has bipyramidal shape and showed LC50 equal to 8.21 x 106 spores/mL. Strain 1644 has cuboid crystal and showed LC50 equal to 2.07 x 106 spores/mL. Comparative analysis of protein profile, plasmid and amplification with primers ERIC allowed the differentiation of the evaluated strains.FERNANDO HERCOS VALICENTE, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS.VALICENTE, F. H.LANA, U. G. de P.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2009-08-1020082016-03-15T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Milho e Sorgo, Sete Lagoas, v. 7, n. 3, p. 195-209, 2008.http://www.alice.cnptia.embrapa.br/alice/handle/doc/491685http://dx.doi.org/10.18512/1980-6477/rbms.v7n3p195-209enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:35:49Zoai:www.alice.cnptia.embrapa.br:doc/491685Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:35:49falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:35:49Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Molecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH). |
title |
Molecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH). |
spellingShingle |
Molecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH). VALICENTE, F. H. Cry genes Bacillus Thuringiensis insect pathology |
title_short |
Molecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH). |
title_full |
Molecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH). |
title_fullStr |
Molecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH). |
title_full_unstemmed |
Molecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH). |
title_sort |
Molecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH). |
author |
VALICENTE, F. H. |
author_facet |
VALICENTE, F. H. LANA, U. G. de P. |
author_role |
author |
author2 |
LANA, U. G. de P. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
FERNANDO HERCOS VALICENTE, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS. |
dc.contributor.author.fl_str_mv |
VALICENTE, F. H. LANA, U. G. de P. |
dc.subject.por.fl_str_mv |
Cry genes Bacillus Thuringiensis insect pathology |
topic |
Cry genes Bacillus Thuringiensis insect pathology |
description |
Bacillus thuringiensis (Berliner) strains 344 and 1644 belonging to the CNPMS/Embrapa (Maize and Sorghum National Research Center) Microorganism Bank, located in Sete Lagoas, MG, showed to be highly efficient in controlling fall armyworm, Spodotpera frugiperda (J.E. Smith). Strains 344 and 1644 were isolated from soil samples originated from Iguassu Falls and Rolândia, respectively, Paraná State, Brazil. While strain 344 harbors cry1Ab, cry1B, cry1E and cry1Fb genes, strain 1644 showed cry1B, cry1C, and cry1D and cry1Fb genes. The strain 344 crystal has bipyramidal shape and showed LC50 equal to 8.21 x 106 spores/mL. Strain 1644 has cuboid crystal and showed LC50 equal to 2.07 x 106 spores/mL. Comparative analysis of protein profile, plasmid and amplification with primers ERIC allowed the differentiation of the evaluated strains. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2009-08-10 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2016-03-15T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 7, n. 3, p. 195-209, 2008. http://www.alice.cnptia.embrapa.br/alice/handle/doc/491685 http://dx.doi.org/10.18512/1980-6477/rbms.v7n3p195-209 |
identifier_str_mv |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 7, n. 3, p. 195-209, 2008. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/491685 http://dx.doi.org/10.18512/1980-6477/rbms.v7n3p195-209 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503341978419200 |