Genomic study of Babesia bovis infection level and its association with tick count in Hereford and Braford cattle.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126663 https://doi.org/10.3389/fimmu.2020.01905 |
Resumo: | Bovine babesiosis is a tick-borne disease caused by intraerythrocytic protozoa and leads to substantial economic losses for the livestock industry throughout the world. Babesia bovis is considered the most pathogenic species, which causes bovine babesiosis in Brazil. Genomic data could be used to evaluate the viability of improving resistance against B. bovis infection level (IB) through genomic selection, and, for that, knowledge of genetic parameters is needed. Furthermore, genome-wide association studies (GWAS) could be conducted to provide a better understanding of the genetic basis of the host response to B. bovis infection. No previous work in quantitative genetics of B. bovis infection was found. Thus, the objective of this study was to estimate the genetic correlation between IB and tick count (TC), evaluate predictive ability and applicability of genomic selection, and perform GWAS in Hereford and Braford cattle. The single-step genomic best linear unbiased prediction method was used, which allows the estimation of both breeding values and marker effects. Standard phenotyping was conducted for both traits. IB quantifications from the blood of 1,858 animals were carried using quantitative PCR assays. For TC, one to three subsequent tick counts were performed by manually counting adult female ticks on one side of each animal's body that was naturally exposed to ticks. Animals were genotyped using the Illumina BovineSNP50 panel. The posterior mean of IB heritability, estimated by the Bayesian animal model in a bivariate analysis, was low (0.10), and the estimations of genetic correlation between IB and TC were also low (0.15). The cross-validation genomic prediction accuracy for IB ranged from 0.18 to 0.35 and from 0.29 to 0.32 using k-means and random clustering, respectively, suggesting that genomic predictions could be used as a tool to improve genetics for IB, especially if a larger training population is developed. The top 10 single nucleotide polymorphisms from the GWAS explained 5.04% of total genetic variance for IB, which were located on chromosomes 1, 2, 5, 6, 12, 17, 18, 16, 24, and 26. Some candidate genes participate in immunity system pathways indicating that those genes are involved in resistance to B. bovis in cattle. Although the genetic correlation between IB and TC was weak, some candidate genes for IB were also reported in tick infestation studies, and they were also involved in biological resistance processes. This study contributes to improving genetic knowledge regarding infection by B. bovis in cattle. |
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Genomic study of Babesia bovis infection level and its association with tick count in Hereford and Braford cattle.Bovine babesiosisCross validationGenetic parametersGenomic selectionGenome wide association studiesBabesia BovisBovine babesiosis is a tick-borne disease caused by intraerythrocytic protozoa and leads to substantial economic losses for the livestock industry throughout the world. Babesia bovis is considered the most pathogenic species, which causes bovine babesiosis in Brazil. Genomic data could be used to evaluate the viability of improving resistance against B. bovis infection level (IB) through genomic selection, and, for that, knowledge of genetic parameters is needed. Furthermore, genome-wide association studies (GWAS) could be conducted to provide a better understanding of the genetic basis of the host response to B. bovis infection. No previous work in quantitative genetics of B. bovis infection was found. Thus, the objective of this study was to estimate the genetic correlation between IB and tick count (TC), evaluate predictive ability and applicability of genomic selection, and perform GWAS in Hereford and Braford cattle. The single-step genomic best linear unbiased prediction method was used, which allows the estimation of both breeding values and marker effects. Standard phenotyping was conducted for both traits. IB quantifications from the blood of 1,858 animals were carried using quantitative PCR assays. For TC, one to three subsequent tick counts were performed by manually counting adult female ticks on one side of each animal's body that was naturally exposed to ticks. Animals were genotyped using the Illumina BovineSNP50 panel. The posterior mean of IB heritability, estimated by the Bayesian animal model in a bivariate analysis, was low (0.10), and the estimations of genetic correlation between IB and TC were also low (0.15). The cross-validation genomic prediction accuracy for IB ranged from 0.18 to 0.35 and from 0.29 to 0.32 using k-means and random clustering, respectively, suggesting that genomic predictions could be used as a tool to improve genetics for IB, especially if a larger training population is developed. The top 10 single nucleotide polymorphisms from the GWAS explained 5.04% of total genetic variance for IB, which were located on chromosomes 1, 2, 5, 6, 12, 17, 18, 16, 24, and 26. Some candidate genes participate in immunity system pathways indicating that those genes are involved in resistance to B. bovis in cattle. Although the genetic correlation between IB and TC was weak, some candidate genes for IB were also reported in tick infestation studies, and they were also involved in biological resistance processes. This study contributes to improving genetic knowledge regarding infection by B. bovis in cattle.LIGIA CAVANI, UNESP; CAMILA URBANO BRAZ, UNESP; RODRIGO GIGLIOTI, Instituto de Zootecnia; CINTIA HIROMI OKINO, CPPSE; CLAUDIA CRISTINA GULIAS GOMES, CPPSUL; ALEXANDRE RODRIGUES CAETANO, Cenargen; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; FERNANDO FLORES CARDOSO, CPPSUL; HENRIQUE NUNES DE OLIVEIRA, UNESP.CAVANI, L.BRAZ, C. U.GIGLIOTI, R.OKINO, C. H.GULIAS GOMES, C. C.CAETANO, A. R.OLIVEIRA, M. C. de S.CARDOSO, F. F.OLIVEIRA, H. N. de2020-11-18T09:13:20Z2020-11-18T09:13:20Z2020-11-172020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11 p.Frontiers in Immunology, v. 11, article 1905, aug. 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126663https://doi.org/10.3389/fimmu.2020.01905enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-11-18T09:13:27Zoai:www.alice.cnptia.embrapa.br:doc/1126663Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-11-18T09:13:27falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-11-18T09:13:27Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genomic study of Babesia bovis infection level and its association with tick count in Hereford and Braford cattle. |
title |
Genomic study of Babesia bovis infection level and its association with tick count in Hereford and Braford cattle. |
spellingShingle |
Genomic study of Babesia bovis infection level and its association with tick count in Hereford and Braford cattle. CAVANI, L. Bovine babesiosis Cross validation Genetic parameters Genomic selection Genome wide association studies Babesia Bovis |
title_short |
Genomic study of Babesia bovis infection level and its association with tick count in Hereford and Braford cattle. |
title_full |
Genomic study of Babesia bovis infection level and its association with tick count in Hereford and Braford cattle. |
title_fullStr |
Genomic study of Babesia bovis infection level and its association with tick count in Hereford and Braford cattle. |
title_full_unstemmed |
Genomic study of Babesia bovis infection level and its association with tick count in Hereford and Braford cattle. |
title_sort |
Genomic study of Babesia bovis infection level and its association with tick count in Hereford and Braford cattle. |
author |
CAVANI, L. |
author_facet |
CAVANI, L. BRAZ, C. U. GIGLIOTI, R. OKINO, C. H. GULIAS GOMES, C. C. CAETANO, A. R. OLIVEIRA, M. C. de S. CARDOSO, F. F. OLIVEIRA, H. N. de |
author_role |
author |
author2 |
BRAZ, C. U. GIGLIOTI, R. OKINO, C. H. GULIAS GOMES, C. C. CAETANO, A. R. OLIVEIRA, M. C. de S. CARDOSO, F. F. OLIVEIRA, H. N. de |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
LIGIA CAVANI, UNESP; CAMILA URBANO BRAZ, UNESP; RODRIGO GIGLIOTI, Instituto de Zootecnia; CINTIA HIROMI OKINO, CPPSE; CLAUDIA CRISTINA GULIAS GOMES, CPPSUL; ALEXANDRE RODRIGUES CAETANO, Cenargen; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; FERNANDO FLORES CARDOSO, CPPSUL; HENRIQUE NUNES DE OLIVEIRA, UNESP. |
dc.contributor.author.fl_str_mv |
CAVANI, L. BRAZ, C. U. GIGLIOTI, R. OKINO, C. H. GULIAS GOMES, C. C. CAETANO, A. R. OLIVEIRA, M. C. de S. CARDOSO, F. F. OLIVEIRA, H. N. de |
dc.subject.por.fl_str_mv |
Bovine babesiosis Cross validation Genetic parameters Genomic selection Genome wide association studies Babesia Bovis |
topic |
Bovine babesiosis Cross validation Genetic parameters Genomic selection Genome wide association studies Babesia Bovis |
description |
Bovine babesiosis is a tick-borne disease caused by intraerythrocytic protozoa and leads to substantial economic losses for the livestock industry throughout the world. Babesia bovis is considered the most pathogenic species, which causes bovine babesiosis in Brazil. Genomic data could be used to evaluate the viability of improving resistance against B. bovis infection level (IB) through genomic selection, and, for that, knowledge of genetic parameters is needed. Furthermore, genome-wide association studies (GWAS) could be conducted to provide a better understanding of the genetic basis of the host response to B. bovis infection. No previous work in quantitative genetics of B. bovis infection was found. Thus, the objective of this study was to estimate the genetic correlation between IB and tick count (TC), evaluate predictive ability and applicability of genomic selection, and perform GWAS in Hereford and Braford cattle. The single-step genomic best linear unbiased prediction method was used, which allows the estimation of both breeding values and marker effects. Standard phenotyping was conducted for both traits. IB quantifications from the blood of 1,858 animals were carried using quantitative PCR assays. For TC, one to three subsequent tick counts were performed by manually counting adult female ticks on one side of each animal's body that was naturally exposed to ticks. Animals were genotyped using the Illumina BovineSNP50 panel. The posterior mean of IB heritability, estimated by the Bayesian animal model in a bivariate analysis, was low (0.10), and the estimations of genetic correlation between IB and TC were also low (0.15). The cross-validation genomic prediction accuracy for IB ranged from 0.18 to 0.35 and from 0.29 to 0.32 using k-means and random clustering, respectively, suggesting that genomic predictions could be used as a tool to improve genetics for IB, especially if a larger training population is developed. The top 10 single nucleotide polymorphisms from the GWAS explained 5.04% of total genetic variance for IB, which were located on chromosomes 1, 2, 5, 6, 12, 17, 18, 16, 24, and 26. Some candidate genes participate in immunity system pathways indicating that those genes are involved in resistance to B. bovis in cattle. Although the genetic correlation between IB and TC was weak, some candidate genes for IB were also reported in tick infestation studies, and they were also involved in biological resistance processes. This study contributes to improving genetic knowledge regarding infection by B. bovis in cattle. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-18T09:13:20Z 2020-11-18T09:13:20Z 2020-11-17 2020 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Frontiers in Immunology, v. 11, article 1905, aug. 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126663 https://doi.org/10.3389/fimmu.2020.01905 |
identifier_str_mv |
Frontiers in Immunology, v. 11, article 1905, aug. 2020. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126663 https://doi.org/10.3389/fimmu.2020.01905 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 p. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503497667837952 |