Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700010 |
Resumo: | Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. |
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Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovisBabesia bovisbabesiosisbovinerecombinant ribosomal phosphoproteinBabesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis.Instituto Oswaldo Cruz, Ministério da Saúde2009-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700010Memórias do Instituto Oswaldo Cruz v.104 n.7 2009reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762009000700010info:eu-repo/semantics/openAccessRamos,Carlos ANAraújo,Flábio RSouza,Ingrid IFOliveira,Renato HMElisei,CarinaSoares,Cleber OSacco,Ana MSRosinha,Grácia MSAlves,Leucio Ceng2020-04-25T17:50:34Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:29.306Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis |
title |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis |
spellingShingle |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis Ramos,Carlos AN Babesia bovis babesiosis bovine recombinant ribosomal phosphoprotein |
title_short |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis |
title_full |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis |
title_fullStr |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis |
title_full_unstemmed |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis |
title_sort |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis |
author |
Ramos,Carlos AN |
author_facet |
Ramos,Carlos AN Araújo,Flábio R Souza,Ingrid IF Oliveira,Renato HM Elisei,Carina Soares,Cleber O Sacco,Ana MS Rosinha,Grácia MS Alves,Leucio C |
author_role |
author |
author2 |
Araújo,Flábio R Souza,Ingrid IF Oliveira,Renato HM Elisei,Carina Soares,Cleber O Sacco,Ana MS Rosinha,Grácia MS Alves,Leucio C |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ramos,Carlos AN Araújo,Flábio R Souza,Ingrid IF Oliveira,Renato HM Elisei,Carina Soares,Cleber O Sacco,Ana MS Rosinha,Grácia MS Alves,Leucio C |
dc.subject.por.fl_str_mv |
Babesia bovis babesiosis bovine recombinant ribosomal phosphoprotein |
topic |
Babesia bovis babesiosis bovine recombinant ribosomal phosphoprotein |
dc.description.none.fl_txt_mv |
Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. |
description |
Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700010 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762009000700010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.104 n.7 2009 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937705594650624 |