Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis

Detalhes bibliográficos
Autor(a) principal: Ramos,Carlos AN
Data de Publicação: 2009
Outros Autores: Araújo,Flábio R, Souza,Ingrid IF, Oliveira,Renato HM, Elisei,Carina, Soares,Cleber O, Sacco,Ana MS, Rosinha,Grácia MS, Alves,Leucio C
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700010
Resumo: Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis.
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spelling Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovisBabesia bovisbabesiosisbovinerecombinant ribosomal phosphoproteinBabesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis.Instituto Oswaldo Cruz, Ministério da Saúde2009-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700010Memórias do Instituto Oswaldo Cruz v.104 n.7 2009reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762009000700010info:eu-repo/semantics/openAccessRamos,Carlos ANAraújo,Flábio RSouza,Ingrid IFOliveira,Renato HMElisei,CarinaSoares,Cleber OSacco,Ana MSRosinha,Grácia MSAlves,Leucio Ceng2020-04-25T17:50:34Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:29.306Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis
title Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis
spellingShingle Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis
Ramos,Carlos AN
Babesia bovis
babesiosis
bovine
recombinant ribosomal phosphoprotein
title_short Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis
title_full Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis
title_fullStr Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis
title_full_unstemmed Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis
title_sort Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis
author Ramos,Carlos AN
author_facet Ramos,Carlos AN
Araújo,Flábio R
Souza,Ingrid IF
Oliveira,Renato HM
Elisei,Carina
Soares,Cleber O
Sacco,Ana MS
Rosinha,Grácia MS
Alves,Leucio C
author_role author
author2 Araújo,Flábio R
Souza,Ingrid IF
Oliveira,Renato HM
Elisei,Carina
Soares,Cleber O
Sacco,Ana MS
Rosinha,Grácia MS
Alves,Leucio C
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ramos,Carlos AN
Araújo,Flábio R
Souza,Ingrid IF
Oliveira,Renato HM
Elisei,Carina
Soares,Cleber O
Sacco,Ana MS
Rosinha,Grácia MS
Alves,Leucio C
dc.subject.por.fl_str_mv Babesia bovis
babesiosis
bovine
recombinant ribosomal phosphoprotein
topic Babesia bovis
babesiosis
bovine
recombinant ribosomal phosphoprotein
dc.description.none.fl_txt_mv Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis.
description Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis.
publishDate 2009
dc.date.none.fl_str_mv 2009-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700010
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762009000700010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.104 n.7 2009
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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