mRNA profile of Nellore calves after primary infection with Haemonchus placei.
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/870087 |
Resumo: | Haemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naïve Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei L3 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-?, MCP-1, lysozyme, pepsinogen and TNF-? genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-? were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-? was down-regulated (p<0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p>0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop. |
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mRNA profile of Nellore calves after primary infection with Haemonchus placei.MRNA profileGastrointestinal parasitesBos IndicusHaemonchus placeiNellorebeef cattlecytokinesHaemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naïve Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei L3 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-?, MCP-1, lysozyme, pepsinogen and TNF-? genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-? were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-? was down-regulated (p<0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p>0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop.ADRIANA M. G. IBELLI, UFSCar/SÃO CARLOS, SP; L. C. NAKATA, UFSCar; R. ANDRÉO, UNESP; L. L. COUTINHO, USP-ESALQ; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; A. F. T. AMARANTE, UNESP; JOHN FURLONG, CNPGL; L. G. ZAROS, UFRGN; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.IBELLI, A. M. G.NAKATA, L. C.ANDRÉO, R.COUTINHO, L. L.OLIVEIRA, M. C. de S.AMARANTE, A. F. T.FURLONG, J.ZAROS, L. G.REGITANO, L. C. de A.2023-03-13T11:51:57Z2023-03-13T11:51:57Z2010-12-152011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVeterinary Parasitology, v. 176, n. 2-3, p. 195-200, mar. 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/87008710.1016/j.vetpar.2010.11.013enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-03-13T11:51:57Zoai:www.alice.cnptia.embrapa.br:doc/870087Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-03-13T11:51:57falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-03-13T11:51:57Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
title |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
spellingShingle |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. IBELLI, A. M. G. MRNA profile Gastrointestinal parasites Bos Indicus Haemonchus placei Nellore beef cattle cytokines |
title_short |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
title_full |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
title_fullStr |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
title_full_unstemmed |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
title_sort |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
author |
IBELLI, A. M. G. |
author_facet |
IBELLI, A. M. G. NAKATA, L. C. ANDRÉO, R. COUTINHO, L. L. OLIVEIRA, M. C. de S. AMARANTE, A. F. T. FURLONG, J. ZAROS, L. G. REGITANO, L. C. de A. |
author_role |
author |
author2 |
NAKATA, L. C. ANDRÉO, R. COUTINHO, L. L. OLIVEIRA, M. C. de S. AMARANTE, A. F. T. FURLONG, J. ZAROS, L. G. REGITANO, L. C. de A. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
ADRIANA M. G. IBELLI, UFSCar/SÃO CARLOS, SP; L. C. NAKATA, UFSCar; R. ANDRÉO, UNESP; L. L. COUTINHO, USP-ESALQ; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; A. F. T. AMARANTE, UNESP; JOHN FURLONG, CNPGL; L. G. ZAROS, UFRGN; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
dc.contributor.author.fl_str_mv |
IBELLI, A. M. G. NAKATA, L. C. ANDRÉO, R. COUTINHO, L. L. OLIVEIRA, M. C. de S. AMARANTE, A. F. T. FURLONG, J. ZAROS, L. G. REGITANO, L. C. de A. |
dc.subject.por.fl_str_mv |
MRNA profile Gastrointestinal parasites Bos Indicus Haemonchus placei Nellore beef cattle cytokines |
topic |
MRNA profile Gastrointestinal parasites Bos Indicus Haemonchus placei Nellore beef cattle cytokines |
description |
Haemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naïve Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei L3 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-?, MCP-1, lysozyme, pepsinogen and TNF-? genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-? were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-? was down-regulated (p<0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p>0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-15 2011 2023-03-13T11:51:57Z 2023-03-13T11:51:57Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Veterinary Parasitology, v. 176, n. 2-3, p. 195-200, mar. 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/870087 10.1016/j.vetpar.2010.11.013 |
identifier_str_mv |
Veterinary Parasitology, v. 176, n. 2-3, p. 195-200, mar. 2011. 10.1016/j.vetpar.2010.11.013 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/870087 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503541125021696 |