mRNA profile of Nellore calves after primary infection with Haemonchus placei

• Autor(a) principal: Ibelli, A. M. G.
• Data de Publicação: 2011
• Outros Autores: Nakata, L. C., Andreo, R. [UNESP], Coutinho, L. L., Oliveira, M. C. S., Amarante, Alessandro Francisco Talamini do [UNESP], Furlong, J., Zaros, L. G., Regitano, L. C. A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.vetpar.2010.11.013; http://hdl.handle.net/11449/18979
• topic: Cytokines; Beef cattle; Haemonchus placei; Bos indicus; Gastrointestinal parasites

Haemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naive Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei 13 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-gamma, MCP-1, lysozyme, pepsinogen and TNF-alpha genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-alpha were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-alpha was down-regulated (p < 0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p > 0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop. (C) 2010 Elsevier B.V. All rights reserved.