Method for obtaining high-resolution proteomic analysis from pericarps of guarana.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/996859 http://dx.doi.org/10.4238/2014.September.29.14 |
Resumo: | Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol. |
id |
EMBR_ec4d4d079cf97f2a083e481215608272 |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/996859 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Method for obtaining high-resolution proteomic analysis from pericarps of guarana.Plant proteomicsTwo-dimensional electrophoresisGuaranáPaullinia CupanapolyphenolsGuarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol.A. L. Souza, UFAM; PAULA CRISTINA DA SILVA ANGELO, CPAA; P. P. O. Nogueira, FIOCRUZ; J. F. C. Gonçalves, INPA; A. M. Franco, INPA; S. Astolfi-Filho, UFAM; J. L. López-Lozano, Fundação de Medicina Tropical do Amazonas; E. V. Andrade, UFAM.SOUZA, A. L.ANGELO, P. C. da S.NOGUEIRA, P. P. O.GONÇALVES, J. F. C.FRANCO, A. M.ASTOLFI-FILHO, S.LÓPEZ-LOZANO, J. L.ANDRADE, E. V.2014-10-09T08:18:15Z2014-10-09T08:18:15Z2014-10-0820142014-10-09T08:18:15Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 13, n. 3, p. 8014-8024, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/996859http://dx.doi.org/10.4238/2014.September.29.14enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:22:15Zoai:www.alice.cnptia.embrapa.br:doc/996859Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:22:15falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:22:15Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Method for obtaining high-resolution proteomic analysis from pericarps of guarana. |
title |
Method for obtaining high-resolution proteomic analysis from pericarps of guarana. |
spellingShingle |
Method for obtaining high-resolution proteomic analysis from pericarps of guarana. SOUZA, A. L. Plant proteomics Two-dimensional electrophoresis Guaraná Paullinia Cupana polyphenols |
title_short |
Method for obtaining high-resolution proteomic analysis from pericarps of guarana. |
title_full |
Method for obtaining high-resolution proteomic analysis from pericarps of guarana. |
title_fullStr |
Method for obtaining high-resolution proteomic analysis from pericarps of guarana. |
title_full_unstemmed |
Method for obtaining high-resolution proteomic analysis from pericarps of guarana. |
title_sort |
Method for obtaining high-resolution proteomic analysis from pericarps of guarana. |
author |
SOUZA, A. L. |
author_facet |
SOUZA, A. L. ANGELO, P. C. da S. NOGUEIRA, P. P. O. GONÇALVES, J. F. C. FRANCO, A. M. ASTOLFI-FILHO, S. LÓPEZ-LOZANO, J. L. ANDRADE, E. V. |
author_role |
author |
author2 |
ANGELO, P. C. da S. NOGUEIRA, P. P. O. GONÇALVES, J. F. C. FRANCO, A. M. ASTOLFI-FILHO, S. LÓPEZ-LOZANO, J. L. ANDRADE, E. V. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
A. L. Souza, UFAM; PAULA CRISTINA DA SILVA ANGELO, CPAA; P. P. O. Nogueira, FIOCRUZ; J. F. C. Gonçalves, INPA; A. M. Franco, INPA; S. Astolfi-Filho, UFAM; J. L. López-Lozano, Fundação de Medicina Tropical do Amazonas; E. V. Andrade, UFAM. |
dc.contributor.author.fl_str_mv |
SOUZA, A. L. ANGELO, P. C. da S. NOGUEIRA, P. P. O. GONÇALVES, J. F. C. FRANCO, A. M. ASTOLFI-FILHO, S. LÓPEZ-LOZANO, J. L. ANDRADE, E. V. |
dc.subject.por.fl_str_mv |
Plant proteomics Two-dimensional electrophoresis Guaraná Paullinia Cupana polyphenols |
topic |
Plant proteomics Two-dimensional electrophoresis Guaraná Paullinia Cupana polyphenols |
description |
Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-09T08:18:15Z 2014-10-09T08:18:15Z 2014-10-08 2014 2014-10-09T08:18:15Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 13, n. 3, p. 8014-8024, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/996859 http://dx.doi.org/10.4238/2014.September.29.14 |
identifier_str_mv |
Genetics and Molecular Research, v. 13, n. 3, p. 8014-8024, 2014. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/996859 http://dx.doi.org/10.4238/2014.September.29.14 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503395053142016 |