Method for obtaining high-resolution proteomic analysis from pericarps of guarana.

• Autor(a) principal: SOUZA, A. L.
• Data de Publicação: 2014
• Outros Autores: ANGELO, P. C. da S., NOGUEIRA, P. P. O., GONÇALVES, J. F. C., FRANCO, A. M., ASTOLFI-FILHO, S., LÓPEZ-LOZANO, J. L., ANDRADE, E. V.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/996859; http://dx.doi.org/10.4238/2014.September.29.14
• topic: Plant proteomics; Two-dimensional electrophoresis; Guaraná; Paullinia Cupana; polyphenols

Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol.