Development of an enzyme-coated microcantilever-based biosensor for specific detection of short-chain alcohols.

• Autor(a) principal: MARGARIDO, A.
• Data de Publicação: 2021
• Outros Autores: MANZINE, L. R., ARAUJO-MOREIRA, F. M., GONÇALVES, R. V., HERRMANN JUNIOR, P. S. de P.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134534; https://doi.org/10.3390/I3S2021Dresden-10175
• topic: Biosensor; Microcantilever; Biorecognition element

This paper describes the development of a biosensor designed for the enzymatic detection of short-chain alcohols. The biorecognition element, alcohol dehydrogenase, was immobilized on selfassembled monolayers deposited on top of silicon nitride microcantilevers. The self-assembly process was performed by surface activation using 3-aminopropyltriethoxysilane, followed by glutaraldehyde and biomolecule binding. X-ray photoelectron spectroscopy and atomic force microscopy were used. The biosensor showed a lower response time and sensibility from 0.03 to 1.2 mL/L. Its selectivity was analyzed through exposure to pure and mixed volatile solvents. Sensor sensibility was higher in the presence of short-chain alcohols and practically null involving other polar or nonpolar solvents.