Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137153 https://doi.org/10.1016/j.fochms.2021.100055 |
Resumo: | The rising consumption of A2 milk and its derivatives in recent years has garnered attention from both consumers and producers, mainly due its possible health benefits, such as enhanced digestion and easier absorption. Thus, a novel real-time PCR using a combination of locked nucleic acid modified (LNA) conjugated probes was developed to genotype A1 and A2 alleles of B-casein gene (CSN2) and to detect and quantify the A1 presence in A2 samples. The limit of detection for each probe (A1 and A2) was evaluated using decreasing serial dilutions. Besides, the sensitivity of A1 allele detection in the A2 samples was also tested. The limits of detection of A1 and A2 alleles were 6 copies, while for A1 allele detection in A2 samples was 7.5 copies (1%). The LNA-probe based method was found to be rapid, robust, highly sensitive, cost effective, and can be employed as screening test to certificate the A2 dairy products. |
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Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples.B CaseinSensitivityLNA probeReal time PCRAllelesThe rising consumption of A2 milk and its derivatives in recent years has garnered attention from both consumers and producers, mainly due its possible health benefits, such as enhanced digestion and easier absorption. Thus, a novel real-time PCR using a combination of locked nucleic acid modified (LNA) conjugated probes was developed to genotype A1 and A2 alleles of B-casein gene (CSN2) and to detect and quantify the A1 presence in A2 samples. The limit of detection for each probe (A1 and A2) was evaluated using decreasing serial dilutions. Besides, the sensitivity of A1 allele detection in the A2 samples was also tested. The limits of detection of A1 and A2 alleles were 6 copies, while for A1 allele detection in A2 samples was 7.5 copies (1%). The LNA-probe based method was found to be rapid, robust, highly sensitive, cost effective, and can be employed as screening test to certificate the A2 dairy products.RODRIGO GIGLIOTI, IZCINTIA HIROMI OKINO, CPPSEBIANCA TAINA AZEVEDO, IZGUNTA GUTMANIS, IZLUCIANA MORITA KATIKI, IZMARCIA CRISTINA DE SENA OLIVEIRA, CPPSEANIBAL EUGENIO VERCESI FILHO, IZ.GIGLIOTI, R.OKINO, C. H.AZEVEDO, B. T.GUTMANIS, G.KATIKI, L. M.OLIVEIRA, M. C. de S.VERCESI FILHO, A. E.2021-12-06T17:00:26Z2021-12-06T17:00:26Z2021-12-062021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6 p.Food Chemistry: Molecular Sciences, v.3, 2021, 100055.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137153https://doi.org/10.1016/j.fochms.2021.100055enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-12-06T17:00:37Zoai:www.alice.cnptia.embrapa.br:doc/1137153Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-12-06T17:00:37falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-12-06T17:00:37Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. |
title |
Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. |
spellingShingle |
Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. GIGLIOTI, R. B Casein Sensitivity LNA probe Real time PCR Alleles |
title_short |
Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. |
title_full |
Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. |
title_fullStr |
Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. |
title_full_unstemmed |
Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. |
title_sort |
Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. |
author |
GIGLIOTI, R. |
author_facet |
GIGLIOTI, R. OKINO, C. H. AZEVEDO, B. T. GUTMANIS, G. KATIKI, L. M. OLIVEIRA, M. C. de S. VERCESI FILHO, A. E. |
author_role |
author |
author2 |
OKINO, C. H. AZEVEDO, B. T. GUTMANIS, G. KATIKI, L. M. OLIVEIRA, M. C. de S. VERCESI FILHO, A. E. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
RODRIGO GIGLIOTI, IZ CINTIA HIROMI OKINO, CPPSE BIANCA TAINA AZEVEDO, IZ GUNTA GUTMANIS, IZ LUCIANA MORITA KATIKI, IZ MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE ANIBAL EUGENIO VERCESI FILHO, IZ. |
dc.contributor.author.fl_str_mv |
GIGLIOTI, R. OKINO, C. H. AZEVEDO, B. T. GUTMANIS, G. KATIKI, L. M. OLIVEIRA, M. C. de S. VERCESI FILHO, A. E. |
dc.subject.por.fl_str_mv |
B Casein Sensitivity LNA probe Real time PCR Alleles |
topic |
B Casein Sensitivity LNA probe Real time PCR Alleles |
description |
The rising consumption of A2 milk and its derivatives in recent years has garnered attention from both consumers and producers, mainly due its possible health benefits, such as enhanced digestion and easier absorption. Thus, a novel real-time PCR using a combination of locked nucleic acid modified (LNA) conjugated probes was developed to genotype A1 and A2 alleles of B-casein gene (CSN2) and to detect and quantify the A1 presence in A2 samples. The limit of detection for each probe (A1 and A2) was evaluated using decreasing serial dilutions. Besides, the sensitivity of A1 allele detection in the A2 samples was also tested. The limits of detection of A1 and A2 alleles were 6 copies, while for A1 allele detection in A2 samples was 7.5 copies (1%). The LNA-probe based method was found to be rapid, robust, highly sensitive, cost effective, and can be employed as screening test to certificate the A2 dairy products. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-06T17:00:26Z 2021-12-06T17:00:26Z 2021-12-06 2021 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Food Chemistry: Molecular Sciences, v.3, 2021, 100055. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137153 https://doi.org/10.1016/j.fochms.2021.100055 |
identifier_str_mv |
Food Chemistry: Molecular Sciences, v.3, 2021, 100055. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137153 https://doi.org/10.1016/j.fochms.2021.100055 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
6 p. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503513418498048 |