Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/880392 |
Resumo: | Coffee seed development is accompanied by severe modifications in water soluble proteins, several of these being associated to a specific developmental stage. For this reason, a proteomic approach has been used to describe spatial-temporal proteome modifications in zygotic embryos at different stages of seed development. Embryos from Coffea arabica seeds were harvested in two different developmental stages: stage 1 at 210 days after anthesis and stage 2 at 255 days. Total proteins were extracted and submitted to 2-DE. From these gels, several spots were identified by mass spectrometry including kinases, MYB transcription factor and enzymes involved in metabolic pathways. All proteins identified seem to affect coffee development in different ways, being directly involved in plant growth or used as an intermediate in some metabolic pathway that, indirectly, will influence coffee development. This is the first work using two-dimensional electrophoresis followed by mass spectrometry analyses that evaluates the expression of proteins during coffee zygotic embryos development. Data here reported supply some light over coffee development and could be used in a near future to improve coffee plants? growth and development by molecular strategies. |
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Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.CoffeeCoffea ArábicaCoffea CanephoraCoffee seed development is accompanied by severe modifications in water soluble proteins, several of these being associated to a specific developmental stage. For this reason, a proteomic approach has been used to describe spatial-temporal proteome modifications in zygotic embryos at different stages of seed development. Embryos from Coffea arabica seeds were harvested in two different developmental stages: stage 1 at 210 days after anthesis and stage 2 at 255 days. Total proteins were extracted and submitted to 2-DE. From these gels, several spots were identified by mass spectrometry including kinases, MYB transcription factor and enzymes involved in metabolic pathways. All proteins identified seem to affect coffee development in different ways, being directly involved in plant growth or used as an intermediate in some metabolic pathway that, indirectly, will influence coffee development. This is the first work using two-dimensional electrophoresis followed by mass spectrometry analyses that evaluates the expression of proteins during coffee zygotic embryos development. Data here reported supply some light over coffee development and could be used in a near future to improve coffee plants? growth and development by molecular strategies.OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; PATRÍCIA B. PELEGRINI, UNIVERSIDADE CATÓLICA DE BRASÍLIA; CLARISSA, P. C. GOMES, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANDRÉA SOUZA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; FABIO T. COSTA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; GILBERTO DUMONT, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; BETANIA FERRAZ QUIRINO, CNPAE; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC.FRANCO, O. L.PELEGRINI, P. B.GOMES, C. P. C.SOUZA, A.COSTA, F. T.DUMONT, G.QUIRINO, B. F.EIRA, M. T. S. daMEHAT, A.2011-04-09T22:27:53Z2011-04-09T22:27:53Z2011-03-1020092011-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlant Physiology and Biochemistry, v. 47, p. 1046-1050, 2009.http://www.alice.cnptia.embrapa.br/alice/handle/doc/880392enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:10:22Zoai:www.alice.cnptia.embrapa.br:doc/880392Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:10:22falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:10:22Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Proteomic evaluation of coffee zygotic embryos in two different stages of seed development. |
title |
Proteomic evaluation of coffee zygotic embryos in two different stages of seed development. |
spellingShingle |
Proteomic evaluation of coffee zygotic embryos in two different stages of seed development. FRANCO, O. L. Coffee Coffea Arábica Coffea Canephora |
title_short |
Proteomic evaluation of coffee zygotic embryos in two different stages of seed development. |
title_full |
Proteomic evaluation of coffee zygotic embryos in two different stages of seed development. |
title_fullStr |
Proteomic evaluation of coffee zygotic embryos in two different stages of seed development. |
title_full_unstemmed |
Proteomic evaluation of coffee zygotic embryos in two different stages of seed development. |
title_sort |
Proteomic evaluation of coffee zygotic embryos in two different stages of seed development. |
author |
FRANCO, O. L. |
author_facet |
FRANCO, O. L. PELEGRINI, P. B. GOMES, C. P. C. SOUZA, A. COSTA, F. T. DUMONT, G. QUIRINO, B. F. EIRA, M. T. S. da MEHAT, A. |
author_role |
author |
author2 |
PELEGRINI, P. B. GOMES, C. P. C. SOUZA, A. COSTA, F. T. DUMONT, G. QUIRINO, B. F. EIRA, M. T. S. da MEHAT, A. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; PATRÍCIA B. PELEGRINI, UNIVERSIDADE CATÓLICA DE BRASÍLIA; CLARISSA, P. C. GOMES, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANDRÉA SOUZA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; FABIO T. COSTA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; GILBERTO DUMONT, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; BETANIA FERRAZ QUIRINO, CNPAE; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC. |
dc.contributor.author.fl_str_mv |
FRANCO, O. L. PELEGRINI, P. B. GOMES, C. P. C. SOUZA, A. COSTA, F. T. DUMONT, G. QUIRINO, B. F. EIRA, M. T. S. da MEHAT, A. |
dc.subject.por.fl_str_mv |
Coffee Coffea Arábica Coffea Canephora |
topic |
Coffee Coffea Arábica Coffea Canephora |
description |
Coffee seed development is accompanied by severe modifications in water soluble proteins, several of these being associated to a specific developmental stage. For this reason, a proteomic approach has been used to describe spatial-temporal proteome modifications in zygotic embryos at different stages of seed development. Embryos from Coffea arabica seeds were harvested in two different developmental stages: stage 1 at 210 days after anthesis and stage 2 at 255 days. Total proteins were extracted and submitted to 2-DE. From these gels, several spots were identified by mass spectrometry including kinases, MYB transcription factor and enzymes involved in metabolic pathways. All proteins identified seem to affect coffee development in different ways, being directly involved in plant growth or used as an intermediate in some metabolic pathway that, indirectly, will influence coffee development. This is the first work using two-dimensional electrophoresis followed by mass spectrometry analyses that evaluates the expression of proteins during coffee zygotic embryos development. Data here reported supply some light over coffee development and could be used in a near future to improve coffee plants? growth and development by molecular strategies. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2011-04-09T22:27:53Z 2011-04-09T22:27:53Z 2011-03-10 2011-04-10T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plant Physiology and Biochemistry, v. 47, p. 1046-1050, 2009. http://www.alice.cnptia.embrapa.br/alice/handle/doc/880392 |
identifier_str_mv |
Plant Physiology and Biochemistry, v. 47, p. 1046-1050, 2009. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/880392 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503340777799680 |