Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.

Detalhes bibliográficos
Autor(a) principal: FRANCO, O. L.
Data de Publicação: 2009
Outros Autores: PELEGRINI, P. B., GOMES, C. P. C., SOUZA, A., COSTA, F. T., DUMONT, G., QUIRINO, B. F., EIRA, M. T. S. da, MEHAT, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/880392
Resumo: Coffee seed development is accompanied by severe modifications in water soluble proteins, several of these being associated to a specific developmental stage. For this reason, a proteomic approach has been used to describe spatial-temporal proteome modifications in zygotic embryos at different stages of seed development. Embryos from Coffea arabica seeds were harvested in two different developmental stages: stage 1 at 210 days after anthesis and stage 2 at 255 days. Total proteins were extracted and submitted to 2-DE. From these gels, several spots were identified by mass spectrometry including kinases, MYB transcription factor and enzymes involved in metabolic pathways. All proteins identified seem to affect coffee development in different ways, being directly involved in plant growth or used as an intermediate in some metabolic pathway that, indirectly, will influence coffee development. This is the first work using two-dimensional electrophoresis followed by mass spectrometry analyses that evaluates the expression of proteins during coffee zygotic embryos development. Data here reported supply some light over coffee development and could be used in a near future to improve coffee plants? growth and development by molecular strategies.
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spelling Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.CoffeeCoffea ArábicaCoffea CanephoraCoffee seed development is accompanied by severe modifications in water soluble proteins, several of these being associated to a specific developmental stage. For this reason, a proteomic approach has been used to describe spatial-temporal proteome modifications in zygotic embryos at different stages of seed development. Embryos from Coffea arabica seeds were harvested in two different developmental stages: stage 1 at 210 days after anthesis and stage 2 at 255 days. Total proteins were extracted and submitted to 2-DE. From these gels, several spots were identified by mass spectrometry including kinases, MYB transcription factor and enzymes involved in metabolic pathways. All proteins identified seem to affect coffee development in different ways, being directly involved in plant growth or used as an intermediate in some metabolic pathway that, indirectly, will influence coffee development. This is the first work using two-dimensional electrophoresis followed by mass spectrometry analyses that evaluates the expression of proteins during coffee zygotic embryos development. Data here reported supply some light over coffee development and could be used in a near future to improve coffee plants? growth and development by molecular strategies.OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; PATRÍCIA B. PELEGRINI, UNIVERSIDADE CATÓLICA DE BRASÍLIA; CLARISSA, P. C. GOMES, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANDRÉA SOUZA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; FABIO T. COSTA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; GILBERTO DUMONT, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; BETANIA FERRAZ QUIRINO, CNPAE; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC.FRANCO, O. L.PELEGRINI, P. B.GOMES, C. P. C.SOUZA, A.COSTA, F. T.DUMONT, G.QUIRINO, B. F.EIRA, M. T. S. daMEHAT, A.2011-04-09T22:27:53Z2011-04-09T22:27:53Z2011-03-1020092011-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlant Physiology and Biochemistry, v. 47, p. 1046-1050, 2009.http://www.alice.cnptia.embrapa.br/alice/handle/doc/880392enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:10:22Zoai:www.alice.cnptia.embrapa.br:doc/880392Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:10:22falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:10:22Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.
title Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.
spellingShingle Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.
FRANCO, O. L.
Coffee
Coffea Arábica
Coffea Canephora
title_short Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.
title_full Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.
title_fullStr Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.
title_full_unstemmed Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.
title_sort Proteomic evaluation of coffee zygotic embryos in two different stages of seed development.
author FRANCO, O. L.
author_facet FRANCO, O. L.
PELEGRINI, P. B.
GOMES, C. P. C.
SOUZA, A.
COSTA, F. T.
DUMONT, G.
QUIRINO, B. F.
EIRA, M. T. S. da
MEHAT, A.
author_role author
author2 PELEGRINI, P. B.
GOMES, C. P. C.
SOUZA, A.
COSTA, F. T.
DUMONT, G.
QUIRINO, B. F.
EIRA, M. T. S. da
MEHAT, A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; PATRÍCIA B. PELEGRINI, UNIVERSIDADE CATÓLICA DE BRASÍLIA; CLARISSA, P. C. GOMES, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANDRÉA SOUZA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; FABIO T. COSTA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; GILBERTO DUMONT, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; BETANIA FERRAZ QUIRINO, CNPAE; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC.
dc.contributor.author.fl_str_mv FRANCO, O. L.
PELEGRINI, P. B.
GOMES, C. P. C.
SOUZA, A.
COSTA, F. T.
DUMONT, G.
QUIRINO, B. F.
EIRA, M. T. S. da
MEHAT, A.
dc.subject.por.fl_str_mv Coffee
Coffea Arábica
Coffea Canephora
topic Coffee
Coffea Arábica
Coffea Canephora
description Coffee seed development is accompanied by severe modifications in water soluble proteins, several of these being associated to a specific developmental stage. For this reason, a proteomic approach has been used to describe spatial-temporal proteome modifications in zygotic embryos at different stages of seed development. Embryos from Coffea arabica seeds were harvested in two different developmental stages: stage 1 at 210 days after anthesis and stage 2 at 255 days. Total proteins were extracted and submitted to 2-DE. From these gels, several spots were identified by mass spectrometry including kinases, MYB transcription factor and enzymes involved in metabolic pathways. All proteins identified seem to affect coffee development in different ways, being directly involved in plant growth or used as an intermediate in some metabolic pathway that, indirectly, will influence coffee development. This is the first work using two-dimensional electrophoresis followed by mass spectrometry analyses that evaluates the expression of proteins during coffee zygotic embryos development. Data here reported supply some light over coffee development and could be used in a near future to improve coffee plants? growth and development by molecular strategies.
publishDate 2009
dc.date.none.fl_str_mv 2009
2011-04-09T22:27:53Z
2011-04-09T22:27:53Z
2011-03-10
2011-04-10T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Plant Physiology and Biochemistry, v. 47, p. 1046-1050, 2009.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/880392
identifier_str_mv Plant Physiology and Biochemistry, v. 47, p. 1046-1050, 2009.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/880392
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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